Rabbit Recombinant Monoclonal p95/NBS1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 26 publications.
Images
![Western blot - Anti-p95/NBS1 antibody [Y112] (AB32074), expandable thumbnail](https://content.abcam.com/products/images/p95-nbs1-antibody-y112-ab32074--western-blot-img164828.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-p95/NBS1 antibody [Y112] (AB32074)")
![Western blot - Anti-p95/NBS1 antibody [Y112] (AB32074), expandable thumbnail](https://content.abcam.com/products/images/p95-nbs1-antibody-y112-ab32074--western-blot-img136251.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-p95/NBS1 antibody [Y112] (AB32074)")
![Western blot - Anti-p95/NBS1 antibody [Y112] (AB32074), expandable thumbnail](https://content.abcam.com/products/images/p95-nbs1-antibody-y112-ab32074--western-blot-img4987.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-p95/NBS1 antibody [Y112] (AB32074)")
![Western blot - Anti-p95/NBS1 antibody [Y112] (AB32074), expandable thumbnail](https://content.abcam.com/products/images/p95-nbs1-antibody-y112-ab32074--western-blot-img61047.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-p95/NBS1 antibody [Y112] (AB32074)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 antibody [Y112] (AB32074), expandable thumbnail](https://content.abcam.com/products/images/p95-nbs1-antibody-y112-ab32074--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img36186.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 antibody [Y112] (AB32074)")
Publications
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- Cell death and differentiation 27:2176-21902020NBS1 is required for SPO11-linked DNA double-strand break repair in male meiosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBin Zhang et. al.PubMed 31965061
- Nucleic acids research 47:9160-91792019CTCF cooperates with CtIP to drive homologous recombination repair of double-strand breaks.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSoon Young Hwang,Mi Ae Kang,Chul Joon Baik,Yejin Lee,Ngo Thanh Hang,Byung-Gyu Kim,Joo Seok Han,Jae-Hoon Jeong,Daechan Park,Kyungjae Myung,Jong-Soo LeePubMed 31340001
- Nature genetics 51:1011-10232019Release of paused RNA polymerase II at specific loci favors DNA double-strand-break formation and promotes cancer translocations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGaetano Ivan Dellino,Fernando Palluzzi,Andrea Maria Chiariello,Rossana Piccioni,Simona Bianco,Laura Furia,Giulia De Conti,Britta A M Bouwman,Giorgio Melloni,Davide Guido,Luciano Giacò,Lucilla Luzi,Davide Cittaro,Mario Faretta,Mario Nicodemi,Nicola Crosetto,Pier Giuseppe PelicciPubMed 31110352
- Marine drugs 17:2019Rat Glioma Cell-Based Functional Characterization of Anti-Stress and Protein Deaggregation Activities in the Marine Carotenoids, Astaxanthin and Fucoxanthin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSajal Afzal et. al.PubMed 30909572
- Molecular cell 74:571-583.e82019MDC1 Interacts with TOPBP1 to Maintain Chromosomal Stability during Mitosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPia-Amata Leimbacher et. al.PubMed 30898438
- Nucleic acids research 47:1294-13102018Perturbing cohesin dynamics drives MRE11 nuclease-dependent replication fork slowing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDenisse Carvajal-Maldonado,Andrea K Byrum,Jessica Jackson,Sarah Wessel,Delphine Lemaçon,Laure Guitton-Sert,Annabel Quinet,Stephanie Tirman,Simona Graziano,Jean-Yves Masson,David Cortez,Susana Gonzalo,Nima Mosammaparast,Alessandro VindigniPubMed 29917110
- Biomedicine & pharmacotherapy = Biomedecine & phar 101:719-7282018Isoorientin triggers apoptosis of hepatoblastoma by inducing DNA double-strand breaks and suppressing homologous recombination repair.
- Cancer cell 33:401-416.e82018BRD4 Inhibition Is Synthetic Lethal with PARP Inhibitors through the Induction of Homologous Recombination Deficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChaoyang Sun,Jun Yin,Yong Fang,Jian Chen,Kang Jin Jeong,Xiaohua Chen,Christopher P Vellano,Zhenlin Ju,Wei Zhao,Dong Zhang,Yiling Lu,Funda Meric-Bernstam,Timothy A Yap,Maureen Hattersley,Mark J O'Connor,Huawei Chen,Stephen Fawell,Shiaw-Yih Lin,Guang Peng,Gordon B MillsPubMed 29533782
- Frontiers in pharmacology 8:7502017Targeting DNA Repair through Podophyllotoxin and Rutin Formulation in Hematopoietic Radioprotection: An Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesM H Yashavarddhan et. al.PubMed 29163150
- Oncotarget 8:42159-421722017Overexpression of karyopherin-α2 in cholangiocarcinoma correlates with poor prognosis and gemcitabine sensitivity via nuclear translocation of DNA repair proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMariko Tsukagoshi,Kenichiro Araki,Takehiko Yokobori,Bolag Altan,Hideki Suzuki,Norio Kubo,Akira Watanabe,Norihiro Ishii,Yasuo Hosouchi,Masahiko Nishiyama,Ken Shirabe,Hiroyuki KuwanoPubMed 28178675
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended |
| Mouse | Predicted | Not recommended | Predicted | Not recommended |
| Rat | Predicted | Not recommended | Predicted | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 | Notes For unpurified use at 1/50. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes For unpurified use at 1/1000 - 1/2000. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes For unpurified use at 1/50. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis (PubMed:10888888, PubMed:15616588, PubMed:18411307, PubMed:18583988, PubMed:18678890, PubMed:19759395, PubMed:23115235, PubMed:28216226, PubMed:28867292, PubMed:9705271). The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases (PubMed:19759395, PubMed:28867292, PubMed:9705271). The complex (1) mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is (2) required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage (PubMed:19759395, PubMed:9705271). The MRN complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11, to initiate end resection, which is required for single-strand invasion and recombination (PubMed:19759395, PubMed:28867292, PubMed:9705271). Within the MRN complex, NBN acts as a protein-protein adapter, which specifically recognizes and binds phosphorylated proteins, promoting their recruitment to DNA damage sites (PubMed:12419185, PubMed:15616588, PubMed:18411307, PubMed:18582474, PubMed:18583988, PubMed:18678890, PubMed:19759395, PubMed:19804756, PubMed:23762398, PubMed:24534091, PubMed:27814491, PubMed:27889449, PubMed:33836577). Recruits MRE11 and RAD50 components of the MRN complex to DSBs in response to DNA damage (PubMed:12419185, PubMed:18411307, PubMed:18583988, PubMed:18678890, PubMed:24534091, PubMed:26438602). Promotes the recruitment of PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites, activating their functions (PubMed:15064416, PubMed:15616588, PubMed:15790808, PubMed:16622404, PubMed:22464731, PubMed:30952868, PubMed:35076389). Mediates the recruitment of phosphorylated RBBP8/CtIP to DSBs, leading to cooperation between the MRN complex and RBBP8/CtIP to initiate end resection (PubMed:19759395, PubMed:27814491, PubMed:27889449, PubMed:33836577). RBBP8/CtIP specifically promotes the endonuclease activity of the MRN complex to clear DNA ends containing protein adducts (PubMed:27814491, PubMed:27889449, PubMed:30787182, PubMed:33836577). The MRN complex is also required for the processing of R-loops (PubMed:31537797). NBN also functions in telomere length maintenance via its interaction with TERF2: interaction with TERF2 during G1 phase preventing recruitment of DCLRE1B/Apollo to telomeres (PubMed:10888888, PubMed:28216226). NBN also promotes DNA repair choice at dysfunctional telomeres: NBN phosphorylation by CK2 promotes non-homologous end joining repair at telomeres, while unphosphorylated NBN promotes microhomology-mediated end-joining (MMEJ) repair (PubMed:28216226). Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex (PubMed:23762398).
Alternative names
NBS, NBS1, P95, NBN, Nibrin, Cell cycle regulatory protein p95, Nijmegen breakage syndrome protein 1, hNbs1
Recommended products
Rabbit Recombinant Monoclonal p95/NBS1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 26 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- Y112
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The target known as p95/NBS1 also referred to as NBN protein is a critical component in the DNA damage response mechanism. The full molecular weight of the NBS1 protein is approximately 95 kDa. This protein is abundantly expressed in various tissues particularly where there is a higher rate of cell division or repair such as in the thymus and testis. It plays an important role in maintaining the stability of the genome through its involvement in the repair of double-strand breaks.
- Biological function summary
The NBS1 protein functions as an essential component of the MRN complex which also includes MRE11 and RAD50 proteins. This complex is fundamental in accurately detecting DNA double-strand breaks and initiating repair processes. Through its actions NBS1 facilitates numerous cellular processes that preserve genomic integrity such as homologous recombination and non-homologous end joining. Its influence in controlling the cell cycle further emphasizes its role in maintaining cellular health.
- Pathways
The NBS1 protein engages significantly in the DNA damage response and repair pathways notably impacting the ATM signaling pathway. By interacting with proteins such as ATM kinase NBS1 facilitates the phosphorylation and activation of several downstream effectors necessary for DNA repair and cell cycle checkpoints. The MRN complex also links with the RAD50 protein highlighting its involvement in these pathways.
- Associated diseases and disorders
Mutations in the NBS1 gene are linked to Nijmegen breakage syndrome characterized by sensitivity to radiation immune deficiency and increased cancer risk. Additionally NBS1's role in pathways makes it pertinent to certain cancer pathologies where its malfunction can contribute to genomic instability. The NBN protein through its interaction with ATM and RAD50 assists in understanding its connection with predispositions to these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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7 product images
![Western blot - Anti-p95/NBS1 antibody [Y112] (ab32074), expandable thumbnail](https://content.abcam.com/products/images/p95-nbs1-antibody-y112-ab32074--western-blot-img164828.jpg "Western blot - Anti-p95/NBS1 antibody [Y112] (ab32074)")
Western blot - Anti-p95/NBS1 antibody [Y112] (ab32074)
Lanes 1 - 2: Merged signal (red and green). Green - ab32074 observed at 90 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.ab32074 was shown to react with p95/NBS1 in wild-type A431 cells in Western blot with loss of signal observed in NBN knockout cell line Human NBN (p95/NBS1) knockout A-431 cell line ab269506 (NBN knockout cell lysate Human NBN (p95/NBS1) knockout A-431 cell lysate ab269668). Wild-type A431 and NBN knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5 % milk in TBS-T (0.1 % Tween®) before incubation with ab32074 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
Lanes 1 - 2: Western blot - Anti-p95/NBS1 antibody [Y112] (ab32074) at 1/1000 dilution
Lanes 1 - 3: Western blot - Anti-p95/NBS1 antibody (Anti-p95/NBS1 antibody ab23996) at 1/1000 dilution
Lane 1: Wild-type A431 cell lysate at 20 µg
Lanes 2 - 3: NBN knockout A431 cell lysate at 20 µg
Lane 2: Western blot - Human NBN (p95/NBS1) knockout A-431 cell line (Human NBN (p95/NBS1) knockout A-431 cell line ab269506)
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 90 kDa, 95 kDa
![Western blot - Anti-p95/NBS1 antibody [Y112] (ab32074), expandable thumbnail](https://content.abcam.com/products/images/p95-nbs1-antibody-y112-ab32074--western-blot-img136251.jpg "Western blot - Anti-p95/NBS1 antibody [Y112] (ab32074)")
Western blot - Anti-p95/NBS1 antibody [Y112] (ab32074)
Lanes 1-3: Merged signal (red and green). Green - ab32074 observed at 95 kDa.ab32074 Anti-p95/NBS1 antibody [Y112] was shown to specifically react with p95/NBS1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human NBN (p95/NBS1) knockout HeLa cell line ab261834 (knockout cell lysate Human NBN (p95/NBS1) knockout HeLa cell lysate ab257111) was used. Wild-type and p95/NBS1 knockout samples were subjected to SDS-PAGE. ab32074, Anti-GAPDH antibody [6C5] - Cytoplasmic Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) and Anti-Histone H3 (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) - Nuclear Loading Control were incubated overnight at 4°C at 1 in 1000 dilution, 1 in 20000 dilution and 1 in 1000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773), Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-p95/NBS1 antibody [Y112] (ab32074) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: NBN knockout HeLa cell lysate at 20 µg
Lane 3: Wild-type HeLa nuclear cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 95 kDa
![Western blot - Anti-p95/NBS1 antibody [Y112] (ab32074), expandable thumbnail](https://content.abcam.com/products/images/p95-nbs1-antibody-y112-ab32074--western-blot-img4987.jpg "Western blot - Anti-p95/NBS1 antibody [Y112] (ab32074)")
Western blot - Anti-p95/NBS1 antibody [Y112] (ab32074)
Lanes 1-3: Merged signal (red and green). Green - ab32074 observed at 95 kDa.ab32074 Anti-p95/NBS1 antibody [Y112] was shown to specifically react with p95/NBS1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human NBN (p95/NBS1) knockout HeLa cell line ab261834 (knockout cell lysate Human NBN (p95/NBS1) knockout HeLa cell lysate ab257111) was used. Wild-type and p95/NBS1 knockout samples were subjected to SDS-PAGE. ab32074, Anti-GAPDH antibody [6C5] - Cytoplasmic Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) and Anti-Histone H3 (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) - Nuclear Loading Control were incubated overnight at 4°C at 1 in 1000 dilution, 1 in 20000 dilution and 1 in 1000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773), Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-p95/NBS1 antibody [Y112] (ab32074) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: NBN knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human NBN (p95/NBS1) knockout HeLa cell line (Human NBN (p95/NBS1) knockout HeLa cell line ab261834)
Lane 3: Wild-type HeLa nuclear cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 95 kDa
![Western blot - Anti-p95/NBS1 antibody [Y112] (ab32074), expandable thumbnail](https://content.abcam.com/products/images/p95-nbs1-antibody-y112-ab32074--western-blot-img61047.jpg "Western blot - Anti-p95/NBS1 antibody [Y112] (ab32074)")
Western blot - Anti-p95/NBS1 antibody [Y112] (ab32074)
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Western blot - Anti-p95/NBS1 antibody [Y112] (ab32074) at 1/1200 dilution
Lane 1: HeLa cell lysate at 20 µg
Lane 2: Jurkat cell lysate at 20 µg
SecondaryAll lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 84 kDa
Observed band size: 95 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 antibody [Y112] (ab32074), expandable thumbnail](https://content.abcam.com/products/images/p95-nbs1-antibody-y112-ab32074--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img36186.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 antibody [Y112] (ab32074)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 antibody [Y112] (ab32074)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling p95/NBS1 with unpurified ab32074 at 1/20. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.
![Western blot - Anti-p95/NBS1 antibody [Y112] (ab32074), expandable thumbnail](https://content.abcam.com/products/images/p95-nbs1-antibody-y112-ab32074--western-blot-img62390.jpg "Western blot - Anti-p95/NBS1 antibody [Y112] (ab32074)")
Western blot - Anti-p95/NBS1 antibody [Y112] (ab32074)
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Western blot - Anti-p95/NBS1 antibody [Y112] (ab32074) at 1/10000 dilution
Lane 1: HeLa cell lysate at 20 µg
Lane 2: Jurkat cell lysate at 20 µg
SecondaryAll lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 84 kDa
Observed band size: 95 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 antibody [Y112] (ab32074), expandable thumbnail](https://content.abcam.com/products/images/p95-nbs1-antibody-y112-ab32074--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128052.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 antibody [Y112] (ab32074)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 antibody [Y112] (ab32074)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling p95/NBS1 with purified ab32074 at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.
![Western blot - Anti-p95/NBS1 antibody [Y112] (ab32074), expandable thumbnail](https://content.abcam.com/products/images/p95-nbs1-antibody-y112-ab32074--western-blot-img62390.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-p95/NBS1 antibody [Y112] (ab32074)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 antibody [Y112] (ab32074), expandable thumbnail](https://content.abcam.com/products/images/p95-nbs1-antibody-y112-ab32074--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128052.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p95/NBS1 antibody [Y112] (ab32074)")
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