Rabbit Polyclonal p95/NBS1 phospho S343 antibody. Suitable for WB and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human NBN phospho S343.
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- Frontiers in immunology 13:7915222022Childhood-Onset Movement Disorders Can Mask a Primary Immunodeficiency: 6 Cases of Classical Ataxia-Telangiectasia and Variant Forms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGeraldine Blanchard-Rohner,Anna Peirolo,Ludivine Coulon,Christian Korff,Judit Horvath,Pierre R Burkhard,Fabienne Gumy-Pause,Emmanuelle Ranza,Peter Jandus,Harpreet Dibra,Alexander Malcolm R Taylor,Joel FlussPubMed 35154108
- Cancers 13:2021Chemotherapy of HER2- and MDM2-Enriched Breast Cancer Subtypes Induces Homologous Recombination DNA Repair and Chemoresistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarcin Herok,Bartosz Wawrzynow,Marta J Maluszek,Maciej B Olszewski,Alicja Zylicz,Maciej ZyliczPubMed 34572735
- The Journal of general virology 101:873-8832020Productive herpesvirus lytic replication in primary effusion lymphoma cells requires S-phase entry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRobert Hollingworth,Grant S Stewart,Roger J GrandPubMed 32501196
- Oncotarget 7:60807-608222016Phenotypic consequences of somatic mutations in the ataxia-telangiectasia mutated gene in non-small cell lung cancer.Applications:WBReactive species:Unspecified reactive speciesAnika Maria Weber,Neele Drobnitzky,Aoife Maire Devery,Sivan Mili Bokobza,Richard A Adams,Timothy S Maughan,Anderson Joseph RyanPubMed 27602502
- PLoS genetics 12:e10059452016A Hypomorphic PALB2 Allele Gives Rise to an Unusual Form of FA-N Associated with Lymphoid Tumour Development.
- Viruses 7:2908-272015Activation of DNA Damage Response Pathways during Lytic Replication of KSHV.Applications:IFReactive species:Unspecified reactive speciesRobert Hollingworth et. al.PubMed 26057167
- PloS one 9:e979692014Direct activation of ATM by resveratrol under oxidizing conditions.
- British journal of cancer 106:262-82011Severe reaction to radiotherapy for breast cancer as the presenting feature of ataxia telangiectasia.Applications:WBReactive species:Unspecified reactive speciesP J Byrd,V Srinivasan,J I Last,A Smith,P Biggs,E F Carney,A Exley,C Abson,G S Stewart,L Izatt,A M TaylorPubMed 22146522
- British journal of cancer 105:586-912011Lymphoid tumours and breast cancer in ataxia telangiectasia; substantial protective effect of residual ATM kinase activity against childhood tumours.
- DNA repair 10:225-342010Requirement for NBS1 in the S phase checkpoint response to DNA methylation combined with PARP inhibition.Applications:WBReactive species:HumanJulie K Horton,Donna F Stefanick,Jennifer Y Zeng,Michael J Carrozza,Samuel H WilsonPubMed 21130714
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human NBN phospho S343. The exact immunogen used to generate this antibody is proprietary information. Database link O60934
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Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes - |
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2 products for Alternative Product
Target data
Function
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis (PubMed:10888888, PubMed:15616588, PubMed:18411307, PubMed:18583988, PubMed:18678890, PubMed:19759395, PubMed:23115235, PubMed:28216226, PubMed:28867292, PubMed:9705271). The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases (PubMed:19759395, PubMed:28867292, PubMed:9705271). The complex (1) mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is (2) required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage (PubMed:19759395, PubMed:9705271). The MRN complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11, to initiate end resection, which is required for single-strand invasion and recombination (PubMed:19759395, PubMed:28867292, PubMed:9705271). Within the MRN complex, NBN acts as a protein-protein adapter, which specifically recognizes and binds phosphorylated proteins, promoting their recruitment to DNA damage sites (PubMed:12419185, PubMed:15616588, PubMed:18411307, PubMed:18582474, PubMed:18583988, PubMed:18678890, PubMed:19759395, PubMed:19804756, PubMed:23762398, PubMed:24534091, PubMed:27814491, PubMed:27889449, PubMed:33836577). Recruits MRE11 and RAD50 components of the MRN complex to DSBs in response to DNA damage (PubMed:12419185, PubMed:18411307, PubMed:18583988, PubMed:18678890, PubMed:24534091, PubMed:26438602). Promotes the recruitment of PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites, activating their functions (PubMed:15064416, PubMed:15616588, PubMed:15790808, PubMed:16622404, PubMed:22464731, PubMed:30952868, PubMed:35076389). Mediates the recruitment of phosphorylated RBBP8/CtIP to DSBs, leading to cooperation between the MRN complex and RBBP8/CtIP to initiate end resection (PubMed:19759395, PubMed:27814491, PubMed:27889449, PubMed:33836577). RBBP8/CtIP specifically promotes the endonuclease activity of the MRN complex to clear DNA ends containing protein adducts (PubMed:27814491, PubMed:27889449, PubMed:30787182, PubMed:33836577). The MRN complex is also required for the processing of R-loops (PubMed:31537797). NBN also functions in telomere length maintenance via its interaction with TERF2: interaction with TERF2 during G1 phase preventing recruitment of DCLRE1B/Apollo to telomeres (PubMed:10888888, PubMed:28216226). NBN also promotes DNA repair choice at dysfunctional telomeres: NBN phosphorylation by CK2 promotes non-homologous end joining repair at telomeres, while unphosphorylated NBN promotes microhomology-mediated end-joining (MMEJ) repair (PubMed:28216226). Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex (PubMed:23762398).
Alternative names
NBS, NBS1, P95, NBN, Nibrin, Cell cycle regulatory protein p95, Nijmegen breakage syndrome protein 1, hNbs1
Recommended products
Rabbit Polyclonal p95/NBS1 phospho S343 antibody. Suitable for WB and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human NBN phospho S343.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human NBN phospho S343. The exact immunogen used to generate this antibody is proprietary information. Database link O60934
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
The antibody against the non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The target known as p95/NBS1 also referred to as NBN protein is a critical component in the DNA damage response mechanism. The full molecular weight of the NBS1 protein is approximately 95 kDa. This protein is abundantly expressed in various tissues particularly where there is a higher rate of cell division or repair such as in the thymus and testis. It plays an important role in maintaining the stability of the genome through its involvement in the repair of double-strand breaks.
- Biological function summary
The NBS1 protein functions as an essential component of the MRN complex which also includes MRE11 and RAD50 proteins. This complex is fundamental in accurately detecting DNA double-strand breaks and initiating repair processes. Through its actions NBS1 facilitates numerous cellular processes that preserve genomic integrity such as homologous recombination and non-homologous end joining. Its influence in controlling the cell cycle further emphasizes its role in maintaining cellular health.
- Pathways
The NBS1 protein engages significantly in the DNA damage response and repair pathways notably impacting the ATM signaling pathway. By interacting with proteins such as ATM kinase NBS1 facilitates the phosphorylation and activation of several downstream effectors necessary for DNA repair and cell cycle checkpoints. The MRN complex also links with the RAD50 protein highlighting its involvement in these pathways.
- Associated diseases and disorders
Mutations in the NBS1 gene are linked to Nijmegen breakage syndrome characterized by sensitivity to radiation immune deficiency and increased cancer risk. Additionally NBS1's role in pathways makes it pertinent to certain cancer pathologies where its malfunction can contribute to genomic instability. The NBN protein through its interaction with ATM and RAD50 assists in understanding its connection with predispositions to these diseases.
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Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
This image is courtesy of an anonymous customer reviewWestern blot - Anti-p95/NBS1 (phospho S343) antibody (ab47272)
All lanes: Western blot - Anti-p95/NBS1 (phospho S343) antibody (ab47272) at 1/500 dilution
Lane 1: Unirradiated Human lymphoblastoid cell lines from normal individual - Whole cell lysate at 50 µg
Lane 2: Irradiated Human lymphoblastoid cell lines from normal individual - Whole cell lysate at 50 µg
Lane 3: Unirradiated Human lymphoblastoid cell lines from classical A-T patient (no ATM kinase expressed) - Whole cell lysate at 50 µg
Lane 4: Irradiated Human lymphoblastoid cell lines from classical A-T patient (no ATM kinase expressed) - Whole cell lysate at 50 µg
SecondaryAll lanes: An HRP-conjugated Goat polyclonal to rabbit IgG at 1/3000 dilution
Predicted band size: 84 kDa
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