Rabbit Polyclonal P97/DAP5 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human EIF4G2 aa 1-250.
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Publications
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- Journal of experimental & clinical cancer research 40:532021MiR-144-3p-mediated dysregulation of EIF4G2 contributes to the development of hepatocellular carcinoma through the ERK pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuangshuang Li et. al.PubMed 33526055
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 59.99% PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human EIF4G2 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P78344
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Tested | Expected |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Rabbit | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Rabbit | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Rabbit | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Product
Target data
Function
Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases.
Alternative names
Eukaryotic translation initiation factor 4 gamma 2, eIF-4-gamma 2, eIF-4G 2, eIF4G 2, Death-associated protein 5, p97, DAP-5, EIF4G2, DAP5, OK/SW-cl.75
Recommended products
Rabbit Polyclonal P97/DAP5 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human EIF4G2 aa 1-250.
Alternative names
Eukaryotic translation initiation factor 4 gamma 2, eIF-4-gamma 2, eIF-4G 2, eIF4G 2, Death-associated protein 5, p97, DAP-5, EIF4G2, DAP5, OK/SW-cl.75
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 59.99% PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human EIF4G2 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P78344
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The P97/DAP5 protein also known as eukaryotic initiation factor 4 gamma 2 (eIF4G2) functions mechanically as a translational regulator. It has a significant role in the process of cap-independent translation where it helps to assemble the initiation complex required for the translation of specific mRNAs. P97/DAP5 has a molecular mass of approximately 97 kDa. It is widely expressed across various tissues including high levels in both neuronal and non-neuronal cells which indicates its importance in fundamental cellular processes.
- Biological function summary
P97/DAP5 impacts translation initiation by interacting with components of the translation machinery. It is part of a multiprotein complex where it binds to other translation initiation factors. Unlike eIF4G1 P97/DAP5 operates in conditions where cap-dependent translation is downregulated such as during cellular stress. This activity highlights its role in selectively activating the translation of mRNAs that are important for cell survival and adaptation during times of cellular distress.
- Pathways
P97/DAP5 plays critical roles in the cellular stress response and apoptosis pathways. In the stress response pathway it is instrumental in shifting translation from a cap-dependent to cap-independent mechanism often in cooperation with proteins like eIF4B. In the apoptosis pathway it modulates the translation of apoptotic factors. These functions ensure cells maintain protein synthesis under adverse conditions maintaining cellular viability and regulating programmed cell death.
- Associated diseases and disorders
P97/DAP5 has links to cancer and neurodegenerative diseases. Alterations in the function or expression of P97/DAP5 can contribute to oncogenesis by allowing cancer cells to survive under stress conditions like hypoxia. It is also related to neurodegenerative disorders due to its significant expression in neurons with dysregulation possibly impacting neuronal survival. Proteins such as eIF4A interplay with P97/DAP5 in these diseases underlining its potential as a therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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7 product images
Western blot - Anti-P97/DAP5 antibody (ab97302)
5% SDS PAGE
All lanes: Western blot - Anti-P97/DAP5 antibody (ab97302) at 1/500 dilution
All lanes: Mouse brain whole cell lysate at 50 µg
Predicted band size: 102 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P97/DAP5 antibody (ab97302)
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft using ab97302 at 1/100 dilution
Immunocytochemistry/ Immunofluorescence - Anti-P97/DAP5 antibody (ab97302)
HeLa cells stained for EIF4G2 (green) using ab97302 at 1/500 dilution in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P97/DAP5 antibody (ab97302)
Paraffin embedded mouse hind brain tissue stained for DAP5 using ab97302 at 1/500 dilution in immunohistochemical analysis.
Western blot - Anti-P97/DAP5 antibody (ab97302)
7.5% SDS PAGE
All lanes: Western blot - Anti-P97/DAP5 antibody (ab97302) at 1/1000 dilution
All lanes: A431 whole cell lysate at 30 µg
Predicted band size: 102 kDa
Immunocytochemistry/ Immunofluorescence - Anti-P97/DAP5 antibody (ab97302)
ICC/IF image of ab97302 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97302, 10μg/ml) overnight at +4°C. The secondary antibody (green) was Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P97/DAP5 antibody (ab97302)
Paraffin embedded human skeletal muscle tissue stained for DAP5 using ab97302 at 10 μg/ml in immunohistochemical analysis.
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