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AB97302

Anti-P97/DAP5 antibody

5

(2 Reviews)

|

(3 Publications)

Rabbit Polyclonal P97/DAP5 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EIF4G2 aa 1-250.

View Alternative Names

DAP5, OK/SW-cl.75, EIF4G2, Eukaryotic translation initiation factor 4 gamma 2, eIF-4-gamma 2, eIF-4G 2, eIF4G 2, Death-associated protein 5, p97, DAP-5

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P97/DAP5 antibody (AB97302)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P97/DAP5 antibody (AB97302)

Paraffin embedded human skeletal muscle tissue stained for DAP5 using ab97302 at 10 μg/ml in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-P97/DAP5 antibody (AB97302)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-P97/DAP5 antibody (AB97302)

HeLa cells stained for EIF4G2 (green) using ab97302 at 1/500 dilution in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P97/DAP5 antibody (AB97302)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P97/DAP5 antibody (AB97302)

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft using ab97302 at 1/100 dilution

Immunocytochemistry/ Immunofluorescence - Anti-P97/DAP5 antibody (AB97302)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-P97/DAP5 antibody (AB97302)

ICC/IF image of ab97302 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97302, 10μg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P97/DAP5 antibody (AB97302)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P97/DAP5 antibody (AB97302)

Paraffin embedded mouse hind brain tissue stained for DAP5 using ab97302 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-P97/DAP5 antibody (AB97302)
  • WB

Unknown

Western blot - Anti-P97/DAP5 antibody (AB97302)

7.5% SDS PAGE

All lanes:

Western blot - Anti-P97/DAP5 antibody (ab97302) at 1/1000 dilution

All lanes:

A431 whole cell lysate at 30 µg

Predicted band size: 102 kDa

false

Western blot - Anti-P97/DAP5 antibody (AB97302)
  • WB

Supplier Data

Western blot - Anti-P97/DAP5 antibody (AB97302)

5% SDS PAGE

All lanes:

Western blot - Anti-P97/DAP5 antibody (ab97302) at 1/500 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 102 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human EIF4G2 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P78344

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The P97/DAP5 protein also known as eukaryotic initiation factor 4 gamma 2 (eIF4G2) functions mechanically as a translational regulator. It has a significant role in the process of cap-independent translation where it helps to assemble the initiation complex required for the translation of specific mRNAs. P97/DAP5 has a molecular mass of approximately 97 kDa. It is widely expressed across various tissues including high levels in both neuronal and non-neuronal cells which indicates its importance in fundamental cellular processes.
Biological function summary

P97/DAP5 impacts translation initiation by interacting with components of the translation machinery. It is part of a multiprotein complex where it binds to other translation initiation factors. Unlike eIF4G1 P97/DAP5 operates in conditions where cap-dependent translation is downregulated such as during cellular stress. This activity highlights its role in selectively activating the translation of mRNAs that are important for cell survival and adaptation during times of cellular distress.

Pathways

P97/DAP5 plays critical roles in the cellular stress response and apoptosis pathways. In the stress response pathway it is instrumental in shifting translation from a cap-dependent to cap-independent mechanism often in cooperation with proteins like eIF4B. In the apoptosis pathway it modulates the translation of apoptotic factors. These functions ensure cells maintain protein synthesis under adverse conditions maintaining cellular viability and regulating programmed cell death.

P97/DAP5 has links to cancer and neurodegenerative diseases. Alterations in the function or expression of P97/DAP5 can contribute to oncogenesis by allowing cancer cells to survive under stress conditions like hypoxia. It is also related to neurodegenerative disorders due to its significant expression in neurons with dysregulation possibly impacting neuronal survival. Proteins such as eIF4A interplay with P97/DAP5 in these diseases underlining its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases.
See full target information EIF4G2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular & cellular proteomics : MCP 24:100960 PubMed40180179

2025

Multi-Omics Analysis Unveils Nsun5-Mediated Molecular Alterations in the Somatosensory Cortex and Its Impact on Pain Sensation.

Applications

Unspecified application

Species

Unspecified reactive species

Peipei Chen,Heyu Zhao,Xia Gao,Junchao Xu,Zhili Huang,Huali Shen

Journal of orthopaedic surgery and research 19:134 PubMed38342889

2024

MicroRNA-877-5p promotes osteoblast differentiation by targeting EIF4G2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

YingChao Shen,Yang Zhang,Qiang Wang,Bo Jiang,XiaoWei Jiang,Bin Luo

Journal of experimental & clinical cancer research 40:53 PubMed33526055

2021

MiR-144-3p-mediated dysregulation of EIF4G2 contributes to the development of hepatocellular carcinoma through the ERK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shuangshuang Li,Jiajia Shao,Guohua Lou,Chao Wu,Yanning Liu,Min Zheng
View all publications
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Product promise

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