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AB308035

Anti-P97/DAP5 antibody [RP23040201]

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Rabbit Recombinant Multiclonal P97/DAP5 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human EIF4G2.

View Alternative Names

DAP5, OK/SW-cl.75, EIF4G2, Eukaryotic translation initiation factor 4 gamma 2, eIF-4-gamma 2, eIF-4G 2, eIF4G 2, Death-associated protein 5, p97, DAP-5

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RP23040201

Isotype

IgG

Carrier free

No

Reacts with

Human, Rat, Mouse

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human EIF4G2.

P78344

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The P97/DAP5 protein also known as eukaryotic initiation factor 4 gamma 2 (eIF4G2) functions mechanically as a translational regulator. It has a significant role in the process of cap-independent translation where it helps to assemble the initiation complex required for the translation of specific mRNAs. P97/DAP5 has a molecular mass of approximately 97 kDa. It is widely expressed across various tissues including high levels in both neuronal and non-neuronal cells which indicates its importance in fundamental cellular processes.
Biological function summary

P97/DAP5 impacts translation initiation by interacting with components of the translation machinery. It is part of a multiprotein complex where it binds to other translation initiation factors. Unlike eIF4G1 P97/DAP5 operates in conditions where cap-dependent translation is downregulated such as during cellular stress. This activity highlights its role in selectively activating the translation of mRNAs that are important for cell survival and adaptation during times of cellular distress.

Pathways

P97/DAP5 plays critical roles in the cellular stress response and apoptosis pathways. In the stress response pathway it is instrumental in shifting translation from a cap-dependent to cap-independent mechanism often in cooperation with proteins like eIF4B. In the apoptosis pathway it modulates the translation of apoptotic factors. These functions ensure cells maintain protein synthesis under adverse conditions maintaining cellular viability and regulating programmed cell death.

P97/DAP5 has links to cancer and neurodegenerative diseases. Alterations in the function or expression of P97/DAP5 can contribute to oncogenesis by allowing cancer cells to survive under stress conditions like hypoxia. It is also related to neurodegenerative disorders due to its significant expression in neurons with dysregulation possibly impacting neuronal survival. Proteins such as eIF4A interplay with P97/DAP5 in these diseases underlining its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases.
See full target information EIF4G2
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Product promise

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