Rabbit Polyclonal PABP antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PABPC1 aa 350 to C-terminus.
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Publications
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- Clinical and translational medicine 12:e9612022Functional genomic analysis of epithelioid sarcoma reveals distinct proximal and distal subtype biology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSamuel V Rasmussen,Jia Xiang Jin,Lissett R Bickford,Andrew D Woods,Felix Sahm,Kenneth A Crawford,Kiyo Nagamori,Hiroaki Goto,Keila E Torres,Angelo Sidoni,Erin R Rudzinski,Khin Thway,Robin L Jones,Alessio Ciulli,Hollis Wright,Melvin Lathara,Ganapati Srinivasa,Kavya Kannan,Paul H Huang,Thomas G P Grünewald,Noah E Berlow,Charles KellerPubMed 35839307
- Nature communications 9:36482018Staufen1 links RNA stress granules and autophagy in a model of neurodegeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSharan Paul,Warunee Dansithong,Karla P Figueroa,Daniel R Scoles,Stefan M PulstPubMed 30194296
- Nucleic acids research 42:7186-2002014DDX6 regulates sequestered nuclear CUG-expanded DMPK-mRNA in dystrophia myotonica type 1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOlof J Pettersson et. al.PubMed 24792155
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PABPC1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P11940
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Binds the poly(A) tail of mRNA, including that of its own transcript, and regulates processes of mRNA metabolism such as pre-mRNA splicing and mRNA stability (PubMed:11051545, PubMed:17212783, PubMed:25480299). Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2 (PubMed:11051545, PubMed:20573744). Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. Binds to N6-methyladenosine (m6A)-containing mRNAs and contributes to MYC stability by binding to m6A-containing MYC mRNAs (PubMed:32245947). Involved in translationally coupled mRNA turnover (PubMed:11051545). Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain (PubMed:11051545). Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons; for the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed (PubMed:18447585). By binding to long poly(A) tails, may protect them from uridylation by ZCCHC6/ZCCHC11 and hence contribute to mRNA stability (PubMed:25480299). (Microbial infection) Positively regulates the replication of dengue virus (DENV).
Alternative names
PAB1, PABP, PABP1, PABPC2, PABPC1, Polyadenylate-binding protein 1, PABP-1, Poly(A)-binding protein 1
Recommended products
Rabbit Polyclonal PABP antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PABPC1 aa 350 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PABPC1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P11940
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PABP also known as Poly(A) Binding Protein is a significant component in mRNA metabolism. It binds to the poly(A) tail of eukaryotic mRNA molecules facilitating the translation process and aiding in mRNA stability. The protein is expressed ubiquitously throughout the cell with a mass of approximately 70 kDa. Some variations like PABPC1 exist in different contexts but the core functions remain consistent across these variations.
- Biological function summary
This protein plays an essential role in translation initiation. PABP interacts with eukaryotic initiation factor 4G (eIF4G) which fosters the formation of the eIF4F complex an assembly critical for ribosome recruitment to mRNA. By linking the 5’ cap structure to the poly(A) tail PABP enhances translational efficiency and RNA integrity. Through these interactions it helps maintain normal cellular function and adapts to changes in the cellular environment.
- Pathways
Poly(A) binding protein is central to the RNA metabolic process. It supports the canonical mRNA decay pathway by interacting with deadenylase complexes which initiate mRNA decay. Another key pathway involves the regulation of nonsense-mediated decay where PABP helps distinguish between normal and aberrant mRNAs. Proteins such as XRN1 and eIF4E closely interact with PABP to execute these pathways efficiently.
- Associated diseases and disorders
Issues with the poly(A) binding protein affect both cancer and neurodegenerative conditions. Aberrant expression or malfunction of PABP leads to defects in mRNA regulation potentially resulting in tumorigenesis due to disrupted cell cycle control. PABP also interacts with proteins like TDP-43 implicated in neurodegenerative diseases affecting RNA processing and potentially contributing to conditions like Amyotrophic Lateral Sclerosis (ALS). The consistent involvement of PABP in these processes highlights its importance to human health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-PABP antibody (ab153930)
7.5% SDS PAGE
All lanes: Western blot - Anti-PABP antibody (ab153930) at 1/1000 dilution
Lane 1: A549 whole cell lysate at 30 µg
Lane 2: H1299 whole cell lysate at 30 µg
Lane 3: HCT116 whole cell lysate at 30 µg
Lane 4: MCF7 whole cell lysate at 30 µg
Predicted band size: 71 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PABP antibody (ab153930)
Immunohistochemical analysis of paraffin-embedded Human hepatoma tissue, labeling PABP with ab153930 at 1/500 dilution.
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