Anti-PABP antibody

7 product images

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  Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody (ab21060)

  ab21060 staining PABP in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab21060 at 1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor^® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

  Also suitable in cells fixed with 4% paraformaldehyde (10 min).

  Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

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  Western blot - Anti-PABP antibody (ab21060)

  ab21060 detects a band of ~ 75 kDa in HeLa whole cell lysate. A 75 kDa band is also seen in Jurkat and A431 whole cell lysates (data not shown). PABP is reported to have run at an apparent molecular mass of 73 kDa on SDS-PAGE. We believe the band we see at 75 kDa represents PABP.

  All lanes: Western blot - Anti-PABP antibody (ab21060) at 1 µg/mL

  All lanes: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg/mL

  Secondary

  All lanes: Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

  Performed under reducing conditions.

  Predicted band size: 71 kDa

  Observed band size: 75 kDa

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  Sharma et al PLoS Pathog. 2017 Oct 30;13(10):e1006677. doi: 10.1371/journal.ppat.1006677. eCollection 2017 Oct. Fig 2. Reproduced under the Creative Commons license https://creativecommons.org/publicdomain/zero/1.0/

  Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody (ab21060)

  Viral ORF57 expression is required to suppress the formation of SG during KSHV lytic infection and under arsenite stress.
  

  ORF57 alone is sufficient to inhibit SG formation. HeLa cells transfected with an ORF57-Flag (pVM7) expressing vector or an empty vector (control) for 24 h were induced with 0.5 mM arsenite for 30 min for SG formation. The cells were stained for ORF57 (green), SG-specific TIA-1 (red) and PABPC1 (white) (ab21060) by each corresponding antibody. The nuclei were counterstained with Hoechst stain. Scale bar = 10 μm.

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  Western blot - Anti-PABP antibody (ab21060)

  All lanes: Western blot - Anti-PABP antibody (ab21060) at 1 µg/mL

  Lane 1: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 10 µg

  Lane 2: MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

  Lane 3: Brain (Mouse) Tissue Lysate at 10 µg

  Lane 4: Western blot - Mouse pancreas tissue lysate - total protein (Mouse pancreas tissue lysate - total protein ab29363) at 10 µg

  Lane 5: Testis (Mouse) Tissue Lysate - normal tissue at 10 µg

  Lane 6: Spinal Cord (Mouse) Tissue Lysate at 10 µg

  Lane 7: Ovary (Mouse) Tissue Lysate - normal tissue at 10 µg

  Lane 8: PC-12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

  Lane 9: Brain (Rat) Tissue Lysate - normal tissue at 10 µg

  Secondary

  All lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

  Performed under reducing conditions.

  Predicted band size: 71 kDa

  Observed band size: 110 kDa, 75 kDa

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  This image is courtesy of an anonymous customer review

  Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody (ab21060)

  ab21060 at 1/50 staining HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by ICC/IF.
  

  HeLa cells fixed for 10 minutes with 1.9 % formaldehyde in 2 % sucrose in PBS then permeabilized for 10 minutes with 0.5% NP40 in PBS-10% sucrose. The cells were then blocked in horse serum and incubated with the antibody for 1 hour. An Alexa Fluor ^® 555 conjugated donkey anti-rabbit antibody was used as the secondary.

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  This image is courtesy of a customer review submitted by Sathya Thulasi Raman.

  Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody (ab21060)

  Immunocytochemical analysis of newborn porcine tracheal epithelial cells, labeling PABP with ab21060.
  

  Cells were formaldehyde-fixed, permeabilized in cold methanol, and blocked with 5% horse serum in PBS for 40 minutes at 23°C. Immunostaining with primary anti-PABP diluted 1/250 for 12 hours at 4°C.

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  This image is courtesy of an anonymous customer review.

  Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody (ab21060)

  Immunocytochemical analysis of rat OLN93 oligodendroglia cells, labeling PABP with ab21060.
  

  Cells were paraformaldehyde-fixed, permeabilized with 0.1% Triton X-100, and blocked with 10% normal goat serum for 1 hour at 20°C. Immunostaining with anti-PABP diluted 1/200 overnight at 4°C.