Anti-PABP antibody (ab21060) is a rabbit polyclonal antibody that is used to detect PABP in Western Blot, ICC/IF. Suitable for Human, Mouse, Rat samples.
- Over 130 publications
- Trusted since 2006
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- Nature communications 14:24092023Large-scale phage-based screening reveals extensive pan-viral mimicry of host short linear motifs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFilip Mihalič,Leandro Simonetti,Girolamo Giudice,Marie Rubin Sander,Richard Lindqvist,Marie Berit Akpiroro Peters,Caroline Benz,Eszter Kassa,Dilip Badgujar,Raviteja Inturi,Muhammad Ali,Izabella Krystkowiak,Ahmed Sayadi,Eva Andersson,Hanna Aronsson,Ola Söderberg,Doreen Dobritzsch,Evangelia Petsalaki,Anna K Överby,Per Jemth,Norman E Davey,Ylva IvarssonPubMed 37100772
- EBioMedicine 91:1045772023Microwave hyperthermia represses human papillomavirus oncoprotein activity and induces cell death due to cell stress in 3D tissue models of anogenital precancers and cancers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichaela J Conley,Ilaria Epifano,Anna Kirk,Andrew Stevenson,Sheila V GrahamPubMed 37068348
- Research (Washington, D.C.) 6:00912023SERBP1 Promotes Stress Granule Clearance by Regulating 26S Proteasome Activity and G3BP1 Ubiquitination and Protects Male Germ Cells from Thermostimuli Damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFengli Wang,Lingjuan Wang,Shiming Gan,Shenglei Feng,Sijin Ouyang,Xiaoli Wang,Shuiqiao YuanPubMed 37223481
- BMC cancer 23:1692023Cytoplasmic poly(A)-binding protein 1 (PABPC1) is a prognostic biomarker to predict survival in nasopharyngeal carcinoma regardless of chemoradiotherapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng Ling,Shengen Xu,Xiaochen Li,Xingwang Sun,Wenbo LongPubMed 36803974
- Nature communications 14:2032023The interferon stimulated gene-encoded protein HELZ2 inhibits human LINE-1 retrotransposition and LINE-1 RNA-mediated type I interferon induction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAhmad Luqman-Fatah,Yuzo Watanabe,Kazuko Uno,Fuyuki Ishikawa,John V Moran,Tomoichiro MiyoshiPubMed 36639706
- Cell reports 41:1115082022Genome-wide CRISPR screen reveals v-ATPase as a drug target to lower levels of ALS protein ataxin-2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGaram Kim,Lisa Nakayama,Jacob A Blum,Tetsuya Akiyama,Steven Boeynaems,Meenakshi Chakraborty,Julien Couthouis,Eduardo Tassoni-Tsuchida,Caitlin M Rodriguez,Michael C Bassik,Aaron D GitlerPubMed 36288714
- Journal of biomedical research 36:255-2682022RNA binding protein BOULE forms aggregates in mammalian testis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYujuan Su,Xinghui Guo,Min Zang,Zhengyao Xie,Tingting Zhao,Eugene Yujun XuPubMed 35965435
- Molecular cell 82:2779-2796.e102022AKT constitutes a signal-promoted alternative exon-junction complex that regulates nonsense-mediated mRNA decay.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHana Cho,Elizabeth T Abshire,Maximilian W Popp,Christoph Pröschel,Joshua L Schwartz,Gene W Yeo,Lynne E MaquatPubMed 35675814
- Nucleic acids research 50:4685-47022022Nuclear and cytoplasmic poly(A) binding proteins (PABPs) favor distinct transcripts and isoforms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAngela L Nicholson-Shaw,Eric R Kofman,Gene W Yeo,Amy E PasquinelliPubMed 35438785
- Nature cell biology 24:299-3062022Pseudouridine-modified tRNA fragments repress aberrant protein synthesis and predict leukaemic progression in myelodysplastic syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNicola Guzzi,Sowndarya Muthukumar,Maciej Cieśla,Gabriele Todisco,Phuong Cao Thi Ngoc,Magdalena Madej,Roberto Munita,Serena Fazio,Simon Ekström,Teresa Mortera-Blanco,Monika Jansson,Yasuhito Nannya,Mario Cazzola,Seishi Ogawa,Luca Malcovati,Eva Hellström-Lindberg,Marios Dimitriou,Cristian BellodiPubMed 35292784
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | WB | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Expected | Tested |
| Rat | Expected | Tested |
| Cow | Predicted | Predicted |
| Pig | Predicted | Predicted |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-5.00000 µg/mL | Notes - |
Species Mouse | Dilution info 1.00000-5.00000 µg/mL | Notes - |
Species Rat | Dilution info 1.00000-2.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig | Dilution info - | Notes - |
Associated Products
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Target data
Function
Binds the poly(A) tail of mRNA, including that of its own transcript, and regulates processes of mRNA metabolism such as pre-mRNA splicing and mRNA stability (PubMed:11051545, PubMed:17212783, PubMed:25480299). Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2 (PubMed:11051545, PubMed:20573744). Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. Binds to N6-methyladenosine (m6A)-containing mRNAs and contributes to MYC stability by binding to m6A-containing MYC mRNAs (PubMed:32245947). Involved in translationally coupled mRNA turnover (PubMed:11051545). Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain (PubMed:11051545). Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons; for the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed (PubMed:18447585). By binding to long poly(A) tails, may protect them from uridylation by ZCCHC6/ZCCHC11 and hence contribute to mRNA stability (PubMed:25480299). (Microbial infection) Positively regulates the replication of dengue virus (DENV).
Alternative names
PAB1, PABP, PABP1, PABPC2, PABPC1, Polyadenylate-binding protein 1, PABP-1, Poly(A)-binding protein 1
Recommended products
Anti-PABP antibody (ab21060) is a rabbit polyclonal antibody that is used to detect PABP in Western Blot, ICC/IF. Suitable for Human, Mouse, Rat samples.
- Over 130 publications
- Trusted since 2006
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab312314.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-PABP antibody (ab21060) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
Trusted by the scientific community
Anti-PABP (ab21060) was first used in a scientific publication in 2006 and has been cited over 130 times in peer-reviewed journals.
Reviewed by scientists
Anti-PABP (ab21060) has over 10 independent reviews from customers.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PABP also known as Poly(A) Binding Protein is a significant component in mRNA metabolism. It binds to the poly(A) tail of eukaryotic mRNA molecules facilitating the translation process and aiding in mRNA stability. The protein is expressed ubiquitously throughout the cell with a mass of approximately 70 kDa. Some variations like PABPC1 exist in different contexts but the core functions remain consistent across these variations.
- Biological function summary
This protein plays an essential role in translation initiation. PABP interacts with eukaryotic initiation factor 4G (eIF4G) which fosters the formation of the eIF4F complex an assembly critical for ribosome recruitment to mRNA. By linking the 5’ cap structure to the poly(A) tail PABP enhances translational efficiency and RNA integrity. Through these interactions it helps maintain normal cellular function and adapts to changes in the cellular environment.
- Pathways
Poly(A) binding protein is central to the RNA metabolic process. It supports the canonical mRNA decay pathway by interacting with deadenylase complexes which initiate mRNA decay. Another key pathway involves the regulation of nonsense-mediated decay where PABP helps distinguish between normal and aberrant mRNAs. Proteins such as XRN1 and eIF4E closely interact with PABP to execute these pathways efficiently.
- Associated diseases and disorders
Issues with the poly(A) binding protein affect both cancer and neurodegenerative conditions. Aberrant expression or malfunction of PABP leads to defects in mRNA regulation potentially resulting in tumorigenesis due to disrupted cell cycle control. PABP also interacts with proteins like TDP-43 implicated in neurodegenerative diseases affecting RNA processing and potentially contributing to conditions like Amyotrophic Lateral Sclerosis (ALS). The consistent involvement of PABP in these processes highlights its importance to human health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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7 product images
Western blot - Anti-PABP antibody (ab21060)
All lanes: Western blot - Anti-PABP antibody (ab21060) at 1 µg/mL
Lane 1: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 10 µg
Lane 2: MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 3: Brain (Mouse) Tissue Lysate at 10 µg
Lane 4: Western blot - Mouse pancreas tissue lysate - total protein (Mouse pancreas tissue lysate - total protein ab29363) at 10 µg
Lane 5: Testis (Mouse) Tissue Lysate - normal tissue at 10 µg
Lane 6: Spinal Cord (Mouse) Tissue Lysate at 10 µg
Lane 7: Ovary (Mouse) Tissue Lysate - normal tissue at 10 µg
Lane 8: PC-12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
Lane 9: Brain (Rat) Tissue Lysate - normal tissue at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 71 kDa
Observed band size: 110 kDa, 75 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody (ab21060)
ab21060 staining PABP in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab21060 at 1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Western blot - Anti-PABP antibody (ab21060)
ab21060 detects a band of ~ 75 kDa in HeLa whole cell lysate. A 75 kDa band is also seen in Jurkat and A431 whole cell lysates (data not shown). PABP is reported to have run at an apparent molecular mass of 73 kDa on SDS-PAGE. We believe the band we see at 75 kDa represents PABP.
All lanes: Western blot - Anti-PABP antibody (ab21060) at 1 µg/mL
All lanes: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg/mL
SecondaryAll lanes: Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 71 kDa
Observed band size: 75 kDa
Sharma et al PLoS Pathog. 2017 Oct 30;13(10):e1006677. doi: 10.1371/journal.ppat.1006677. eCollection 2017 Oct. Fig 2. Reproduced under the Creative Commons license https://creativecommons.org/publicdomain/zero/1.0/Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody (ab21060)
Viral ORF57 expression is required to suppress the formation of SG during KSHV lytic infection and under arsenite stress.ORF57 alone is sufficient to inhibit SG formation. HeLa cells transfected with an ORF57-Flag (pVM7) expressing vector or an empty vector (control) for 24 h were induced with 0.5 mM arsenite for 30 min for SG formation. The cells were stained for ORF57 (green), SG-specific TIA-1 (red) and PABPC1 (white) (ab21060) by each corresponding antibody. The nuclei were counterstained with Hoechst stain. Scale bar = 10 μm.
This image is courtesy of an anonymous customer reviewImmunocytochemistry/ Immunofluorescence - Anti-PABP antibody (ab21060)
ab21060 at 1/50 staining HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by ICC/IF.
HeLa cells fixed for 10 minutes with 1.9 % formaldehyde in 2 % sucrose in PBS then permeabilized for 10 minutes with 0.5% NP40 in PBS-10% sucrose. The cells were then blocked in horse serum and incubated with the antibody for 1 hour. An Alexa Fluor ® 555 conjugated donkey anti-rabbit antibody was used as the secondary.
This image is courtesy of a customer review submitted by Sathya Thulasi Raman.Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody (ab21060)
Immunocytochemical analysis of newborn porcine tracheal epithelial cells, labeling PABP with ab21060.
Cells were formaldehyde-fixed, permeabilized in cold methanol, and blocked with 5% horse serum in PBS for 40 minutes at 23°C. Immunostaining with primary anti-PABP diluted 1/250 for 12 hours at 4°C.
This image is courtesy of an anonymous customer review.Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody (ab21060)
Immunocytochemical analysis of rat OLN93 oligodendroglia cells, labeling PABP with ab21060.
Cells were paraformaldehyde-fixed, permeabilized with 0.1% Triton X-100, and blocked with 10% normal goat serum for 1 hour at 20°C. Immunostaining with anti-PABP diluted 1/200 overnight at 4°C.
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