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Rabbit Recombinant Monoclonal PABP antibody. Suitable for WB, ICC/IF, IP, Flow Cyt (Intra), Dot and reacts with Human, Mouse, Rat, Synthetic peptide samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody [EPR27188-86] (AB312314), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-PABP antibody [EPR27188-86] (AB312314), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody [EPR27188-86] (AB312314), expandable thumbnail
  • Immunoprecipitation - Anti-PABP antibody [EPR27188-86] (AB312314), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody [EPR27188-86] (AB312314), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIPFlow Cyt (Intra)DotIHC-P
Human
Tested
Tested
Tested
Tested
Expected
Not recommended
Mouse
Tested
Tested
Expected
Tested
Expected
Not recommended
Rat
Tested
Tested
Expected
Tested
Expected
Not recommended
Synthetic peptide
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Species
Mouse
Dilution info
1/100
Notes

-

Species
Rat
Dilution info
1/100
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Species
Mouse
Dilution info
1/500
Notes

-

Species
Rat
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human, Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Rat, Human, Mouse, Synthetic peptide
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Binds the poly(A) tail of mRNA, including that of its own transcript, and regulates processes of mRNA metabolism such as pre-mRNA splicing and mRNA stability (PubMed:11051545, PubMed:17212783, PubMed:25480299). Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2 (PubMed:11051545, PubMed:20573744). Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. Binds to N6-methyladenosine (m6A)-containing mRNAs and contributes to MYC stability by binding to m6A-containing MYC mRNAs (PubMed:32245947). Involved in translationally coupled mRNA turnover (PubMed:11051545). Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain (PubMed:11051545). Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons; for the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed (PubMed:18447585). By binding to long poly(A) tails, may protect them from uridylation by ZCCHC6/ZCCHC11 and hence contribute to mRNA stability (PubMed:25480299). (Microbial infection) Positively regulates the replication of dengue virus (DENV).

Alternative names

PAB1, PABP, PABP1, PABPC2, PABPC1, Polyadenylate-binding protein 1, PABP-1, Poly(A)-binding protein 1

Recommended products

Rabbit Recombinant Monoclonal PABP antibody. Suitable for WB, ICC/IF, IP, Flow Cyt (Intra), Dot and reacts with Human, Mouse, Rat, Synthetic peptide samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27188-86
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PABP also known as Poly(A) Binding Protein is a significant component in mRNA metabolism. It binds to the poly(A) tail of eukaryotic mRNA molecules facilitating the translation process and aiding in mRNA stability. The protein is expressed ubiquitously throughout the cell with a mass of approximately 70 kDa. Some variations like PABPC1 exist in different contexts but the core functions remain consistent across these variations.

Biological function summary

This protein plays an essential role in translation initiation. PABP interacts with eukaryotic initiation factor 4G (eIF4G) which fosters the formation of the eIF4F complex an assembly critical for ribosome recruitment to mRNA. By linking the 5’ cap structure to the poly(A) tail PABP enhances translational efficiency and RNA integrity. Through these interactions it helps maintain normal cellular function and adapts to changes in the cellular environment.

Pathways

Poly(A) binding protein is central to the RNA metabolic process. It supports the canonical mRNA decay pathway by interacting with deadenylase complexes which initiate mRNA decay. Another key pathway involves the regulation of nonsense-mediated decay where PABP helps distinguish between normal and aberrant mRNAs. Proteins such as XRN1 and eIF4E closely interact with PABP to execute these pathways efficiently.

Associated diseases and disorders

Issues with the poly(A) binding protein affect both cancer and neurodegenerative conditions. Aberrant expression or malfunction of PABP leads to defects in mRNA regulation potentially resulting in tumorigenesis due to disrupted cell cycle control. PABP also interacts with proteins like TDP-43 implicated in neurodegenerative diseases affecting RNA processing and potentially contributing to conditions like Amyotrophic Lateral Sclerosis (ALS). The consistent involvement of PABP in these processes highlights its importance to human health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody [EPR27188-86] (ab312314), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody [EPR27188-86] (ab312314)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-12 (rat adrenal gland pheochromocytoma cell) cells labelling PABP with ab312314 at 1/100 (10.36 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in PC-12 cell line. The image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Flow Cytometry (Intracellular) - Anti-PABP antibody [EPR27188-86] (ab312314), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PABP antibody [EPR27188-86] (ab312314)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling PABP with ab312314 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody [EPR27188-86] (ab312314), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody [EPR27188-86] (ab312314)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C2C12 (mouse myoblast) cells labelling PABP with ab312314 at 1/100 (10.36 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in undifferenced C2C12 cells and the expression was decreased in differentiated C2C12 cells. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunoprecipitation - Anti-PABP antibody [EPR27188-86] (ab312314), expandable thumbnail

    Immunoprecipitation - Anti-PABP antibody [EPR27188-86] (ab312314)

    PABP was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab312314 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab312314 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
    Lane 2: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab312314 in HeLa whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 15 seconds

    All lanes: Immunoprecipitation - Anti-PABP antibody [EPR27188-86] (ab312314) at 1/30 dilution

    All lanes: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 10 µg

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 15s

  • Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody [EPR27188-86] (ab312314), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody [EPR27188-86] (ab312314)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling PABP with ab312314 at 1/100 (10.36 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in HeLa cell line. The image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Flow Cytometry (Intracellular) - Anti-PABP antibody [EPR27188-86] (ab312314), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PABP antibody [EPR27188-86] (ab312314)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C2C12 (mouse myoblast) treated with differentiation to muscle for 6 days (Red) / untreated C2C12 cells (Green) cells labelling PABP with ab312314 at 1/500 dilution (0.1 ug)/Red and Green (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-PABP antibody [EPR27188-86] (ab312314), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PABP antibody [EPR27188-86] (ab312314)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized PC-12 (rat adrenal gland pheochromocytoma cell) cells labelling PABP with ab312314 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Western blot - Anti-PABP antibody [EPR27188-86] (ab312314), expandable thumbnail

    Western blot - Anti-PABP antibody [EPR27188-86] (ab312314)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    The expression of PABP was decreased in differentiated C2C12 cells (PMID:28653618).
    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
    Exposure time: 180 seconds

    All lanes: Western blot - Anti-PABP antibody [EPR27188-86] (ab312314) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: LNCaP (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: Undifferenced C2C12 (mouse myoblast) whole cell lysate at 20 µg

    Lane 5: C2C12 differentiated to muscle for 6 days whole cell lysate at 20 µg

    Lane 6: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 7: RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Lane 8: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 71 kDa

    Exposure time: 180s

  • Dot Blot - Anti-PABP antibody [EPR27188-86] (ab312314), expandable thumbnail

    Dot Blot - Anti-PABP antibody [EPR27188-86] (ab312314)

    Dot blot analysis of PABP using ab312314 at 1:1000 (0.518 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
    Lane 1: PABP peptide
    Lane 2: PABP3 peptide
    Exposure time: 180 seconds.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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