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Rabbit Recombinant Monoclonal PABP antibody. Carrier free. Suitable for WB, ICC/IF, IP, Flow Cyt (Intra), Dot and reacts with Human, Mouse, Rat, Synthetic peptide samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody [EPR27188-86] - BSA and Azide free (AB312315), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-PABP antibody [EPR27188-86] - BSA and Azide free (AB312315), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody [EPR27188-86] - BSA and Azide free (AB312315), expandable thumbnail
  • Immunoprecipitation - Anti-PABP antibody [EPR27188-86] - BSA and Azide free (AB312315), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody [EPR27188-86] - BSA and Azide free (AB312315), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIPFlow Cyt (Intra)DotIHC-P
Human
Tested
Tested
Tested
Tested
Expected
Not recommended
Mouse
Tested
Tested
Expected
Tested
Expected
Not recommended
Rat
Tested
Tested
Expected
Tested
Expected
Not recommended
Synthetic peptide
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human, Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Synthetic peptide
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Binds the poly(A) tail of mRNA, including that of its own transcript, and regulates processes of mRNA metabolism such as pre-mRNA splicing and mRNA stability (PubMed:11051545, PubMed:17212783, PubMed:25480299). Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2 (PubMed:11051545, PubMed:20573744). Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. Binds to N6-methyladenosine (m6A)-containing mRNAs and contributes to MYC stability by binding to m6A-containing MYC mRNAs (PubMed:32245947). Involved in translationally coupled mRNA turnover (PubMed:11051545). Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain (PubMed:11051545). Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons; for the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed (PubMed:18447585). By binding to long poly(A) tails, may protect them from uridylation by ZCCHC6/ZCCHC11 and hence contribute to mRNA stability (PubMed:25480299). (Microbial infection) Positively regulates the replication of dengue virus (DENV).

Alternative names

PAB1, PABP, PABP1, PABPC2, PABPC1, Polyadenylate-binding protein 1, PABP-1, Poly(A)-binding protein 1

Recommended products

Rabbit Recombinant Monoclonal PABP antibody. Carrier free. Suitable for WB, ICC/IF, IP, Flow Cyt (Intra), Dot and reacts with Human, Mouse, Rat, Synthetic peptide samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR27188-86
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PABP also known as Poly(A) Binding Protein is a significant component in mRNA metabolism. It binds to the poly(A) tail of eukaryotic mRNA molecules facilitating the translation process and aiding in mRNA stability. The protein is expressed ubiquitously throughout the cell with a mass of approximately 70 kDa. Some variations like PABPC1 exist in different contexts but the core functions remain consistent across these variations.

Biological function summary

This protein plays an essential role in translation initiation. PABP interacts with eukaryotic initiation factor 4G (eIF4G) which fosters the formation of the eIF4F complex an assembly critical for ribosome recruitment to mRNA. By linking the 5’ cap structure to the poly(A) tail PABP enhances translational efficiency and RNA integrity. Through these interactions it helps maintain normal cellular function and adapts to changes in the cellular environment.

Pathways

Poly(A) binding protein is central to the RNA metabolic process. It supports the canonical mRNA decay pathway by interacting with deadenylase complexes which initiate mRNA decay. Another key pathway involves the regulation of nonsense-mediated decay where PABP helps distinguish between normal and aberrant mRNAs. Proteins such as XRN1 and eIF4E closely interact with PABP to execute these pathways efficiently.

Associated diseases and disorders

Issues with the poly(A) binding protein affect both cancer and neurodegenerative conditions. Aberrant expression or malfunction of PABP leads to defects in mRNA regulation potentially resulting in tumorigenesis due to disrupted cell cycle control. PABP also interacts with proteins like TDP-43 implicated in neurodegenerative diseases affecting RNA processing and potentially contributing to conditions like Amyotrophic Lateral Sclerosis (ALS). The consistent involvement of PABP in these processes highlights its importance to human health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com