AB241477

Anti-PABPN1 antibody

Name: Anti-PABPN1 antibody (ab241477) | Abcam
Brand: Abcam Limited
SKU: AB241477
Price: 510 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal PABPN1 antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PABPN1 aa 1-50.

View Alternative Names

PAB2, PABP2, PABPN1, Polyadenylate-binding protein 2, PABP-2, Poly(A)-binding protein 2, Nuclear poly(A)-binding protein 1, Poly(A)-binding protein II, Polyadenylate-binding nuclear protein 1, PABII

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PABPN1 antibody (AB241477)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PABPN1 antibody (AB241477)

Formalin-fixed, paraffin-embedded human stomach carcinoma tissue stained for PABPN1 using ab241477 at 1/1000 dilution in immunohistochemical analysis. Detection : DAB staining.

Supplier Data

Immunoprecipitation - Anti-PABPN1 antibody (AB241477)

PABPN1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241477 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab241477 at 0.4 μg/ml.

Lane 1 : ab241477 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 1 second.

All lanes:

Immunoprecipitation - Anti-PABPN1 antibody (ab241477)

Predicted band size: 33 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PABPN1 antibody (AB241477)

Formalin-fixed, paraffin-embedded mouse teratoma tissue stained for PABPN1 using ab241477 at 1/200 dilution in immunohistochemical analysis. Detection : DAB staining.

Supplier Data

Western blot - Anti-PABPN1 antibody (AB241477)

All lanes:

Western blot - Anti-PABPN1 antibody (ab241477) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 4:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 33 kDa

true

Exposure time: 3s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB, IP

applications

Immunogen

Synthetic Peptide within Human PABPN1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q86U42

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab241477 was affinity purified using an epitope specific to PABPN1 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PABPN1 also known as polyadenylate-binding nuclear protein 1 is an RNA-binding protein that plays a fundamental role in the regulation of mRNA processing. It is a 31-kilodalton protein that binds to the poly(A) tail of pre-mRNA and stimulates the assembly of the polyadenylation complex thereby influencing the maturation and stability of mRNA transcripts. This protein is predominantly expressed in the nucleus of eukaryotic cells where it performs its important function in post-transcriptional regulation.

Biological function summary

PABPN1 is involved in controlling the polyadenylation process of pre-mRNA which is essential for producing mature mRNA molecules. PABPN1 operates within a multi-protein complex that includes enzymes like poly(A) polymerase and cleavage stimulation factor (CstF). By interacting with these proteins PABPN1 ensures the correct formation of the 3' end of mRNA influencing gene expression regulation and mRNA stability.

Pathways

PABPN1 participates in key cellular pathways such as mRNA processing and nuclear export of mRNA. It operates in concert with other proteins like PABPC1 which aids in the transition of mRNA from the nucleus to the cytoplasm. The protein maintains the balance of mRNA stability and translation efficiency which are critical aspects of efficient gene expression.

PABPN1 mutations are linked to oculopharyngeal muscular dystrophy (OPMD) a genetic condition that leads to muscle weakness. Another related disorder involves its interaction with proteins like HSP70 where its dysfunction may contribute to disrupted protein homeostasis in affected tissues. Studying PABPN1's role in these diseases provides insight into the molecular mechanisms underlying these conditions and aids in the development of potential therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product (By similarity). Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length (By similarity). Increases the affinity of poly(A) polymerase for RNA (By similarity). Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes (PubMed : 11371506). Binds to poly(A) and to poly(G) with high affinity (By similarity). May protect the poly(A) tail from degradation (By similarity). Subunit of the trimeric poly(A) tail exosome targeting (PAXT) complex, a complex that directs a subset of long and polyadenylated poly(A) RNAs for exosomal degradation. The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its cofactor MTREX, which links to RNA-binding protein adapters (PubMed : 27871484).

See full target information PABPN1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 20:3956-3971 PubMed39113702

2024

PKCα Induced the Generation of Extracellular Vesicles in Activated Platelets to Promote Breast Cancer Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Jinghua Zhao,Huan Tian,Xiaona Zhao,Lan Lan,Huanhuan Liu,Yi Sun,Fengyan Yu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PABPN1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PABPN1 antibody (AB241477)

Immunoprecipitation - Anti-PABPN1 antibody (AB241477)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PABPN1 antibody (AB241477)

Western blot - Anti-PABPN1 antibody (AB241477)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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