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Rabbit Monoclonal PABPN1 antibody. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.


Images

Western blot - Anti-PABPN1 antibody [EP3001Y] (AB81224), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PABPN1 antibody [EP3001Y] (AB81224), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-PABPN1 antibody [EP3001Y] (AB81224), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PFlow Cyt (Intra)
Human
Not recommended
Tested
Tested
Tested
Mouse
Not recommended
Predicted
Predicted
Predicted
Cow
Not recommended
Predicted
Predicted
Predicted

Not recommended
Not recommended

Species
Human, Mouse, Cow
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10000.00000 - 1/50000.00000
Notes

-

Predicted
Predicted

Species
Mouse, Cow
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100.00000 - 1/250.00000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Cow
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/60
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Mouse, Cow
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product (By similarity). Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length (By similarity). Increases the affinity of poly(A) polymerase for RNA (By similarity). Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes (PubMed:11371506). Binds to poly(A) and to poly(G) with high affinity (By similarity). May protect the poly(A) tail from degradation (By similarity). Subunit of the trimeric poly(A) tail exosome targeting (PAXT) complex, a complex that directs a subset of long and polyadenylated poly(A) RNAs for exosomal degradation. The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its cofactor MTREX, which links to RNA-binding protein adapters (PubMed:27871484).

Alternative names

PAB2, PABP2, PABPN1, Polyadenylate-binding protein 2, PABP-2, Poly(A)-binding protein 2, Nuclear poly(A)-binding protein 1, Poly(A)-binding protein II, Polyadenylate-binding nuclear protein 1, PABII

Recommended products

Rabbit Monoclonal PABPN1 antibody. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP3001Y
Purity
Tissue culture supernatant
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PABPN1 also known as polyadenylate-binding nuclear protein 1 is an RNA-binding protein that plays a fundamental role in the regulation of mRNA processing. It is a 31-kilodalton protein that binds to the poly(A) tail of pre-mRNA and stimulates the assembly of the polyadenylation complex thereby influencing the maturation and stability of mRNA transcripts. This protein is predominantly expressed in the nucleus of eukaryotic cells where it performs its important function in post-transcriptional regulation.

Biological function summary

PABPN1 is involved in controlling the polyadenylation process of pre-mRNA which is essential for producing mature mRNA molecules. PABPN1 operates within a multi-protein complex that includes enzymes like poly(A) polymerase and cleavage stimulation factor (CstF). By interacting with these proteins PABPN1 ensures the correct formation of the 3' end of mRNA influencing gene expression regulation and mRNA stability.

Pathways

PABPN1 participates in key cellular pathways such as mRNA processing and nuclear export of mRNA. It operates in concert with other proteins like PABPC1 which aids in the transition of mRNA from the nucleus to the cytoplasm. The protein maintains the balance of mRNA stability and translation efficiency which are critical aspects of efficient gene expression.

Associated diseases and disorders

PABPN1 mutations are linked to oculopharyngeal muscular dystrophy (OPMD) a genetic condition that leads to muscle weakness. Another related disorder involves its interaction with proteins like HSP70 where its dysfunction may contribute to disrupted protein homeostasis in affected tissues. Studying PABPN1's role in these diseases provides insight into the molecular mechanisms underlying these conditions and aids in the development of potential therapeutic strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-PABPN1 antibody [EP3001Y] (ab81224), expandable thumbnail

    Western blot - Anti-PABPN1 antibody [EP3001Y] (ab81224)

    All lanes: Western blot - Anti-PABPN1 antibody [EP3001Y] (ab81224) at 1/20000 dilution

    Lane 1: T47D cell lysates at 10 µg

    Lane 2: SKBR3 cell lysates at 10 µg

    Lane 3: Ramos cell lysates at 10 µg

    Lane 4: Raji cell lysates at 10 µg

    Secondary

    All lanes: HRP labelled goat anti rabbit at 1/2000 dilution

    Predicted band size: 33 kDa

    Observed band size: 50 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PABPN1 antibody [EP3001Y] (ab81224), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PABPN1 antibody [EP3001Y] (ab81224)

    Immunohistochemistry staining of paraffin-embedded urinary bladder transitional cell carcinoma tissue, using 1/100 ab81224

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-PABPN1 antibody [EP3001Y] (ab81224), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PABPN1 antibody [EP3001Y] (ab81224)

    Overlay histogram showing HeLa cells stained with ab81224 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81224, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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