AB172909

Anti-PAC-2 antibody [EPR9947(2)]

Name: Anti-PAC-2 antibody [EPR9947(2)] (ab172909) | Abcam
Brand: Abcam Limited
SKU: AB172909
Price: 340 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(4 Publications)

Rabbit Recombinant Monoclonal PAC-2 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

HCCA3, PAC2, TNFSF5IP1, PSMG2, Proteasome assembly chaperone 2, PAC-2, Hepatocellular carcinoma-susceptibility protein 3, Tumor necrosis factor superfamily member 5-induced protein 1

1 Images

Supplier Data

Western blot - Anti-PAC-2 antibody [EPR9947(2)] (AB172909)

All lanes:

Western blot - Anti-PAC-2 antibody [EPR9947(2)] (ab172909) at 1/1000 dilution

Lane 1:

Caco-2 cell lysate

Lane 2:

HepG2 cell lysate

Lane 3:

HeLa cell lysate

Lane 4:

Jurkat cell lysate

Predicted band size: 29 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9947(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PAC-2 also known as PAC and Anti-PAC is a protein involved in various cellular processes. It appears important in apoptosis and the regulation of cellular proliferation. PAC-2 has an approximate mass of 40 kDa. Researchers have observed its expression in different tissues including the nervous system immune cells and several cancerous tissues. Understanding the mechanical functions of PAC-2 can offer insights into its role in cellular homeostasis and disease pathology.

Biological function summary

PAC-2 plays an essential role in apoptosis acting as a mediator of programmed cell death. While not characterized as part of a larger complex PAC-2 interacts with other proteins to regulate cell survival and death. It likely influences the mitochondrial pathway of apoptosis by controlling cytochrome c release which is a critical step in the activation of caspases. These interactions emphasize the protein's adaptive importance in various biological contexts.

Pathways

PAC-2 commands a significant presence in apoptotic signaling and cellular stress response pathways. It influences the intrinsic (mitochondrial) apoptotic pathway and may interact with proteins such as Bcl-2 family members and caspase-9. This interaction places PAC-2 in an important position where it can modify the balance between cell survival and cell death involving proteins that balance pro-apoptotic and anti-apoptotic signals.

PAC-2 has correlations with cancer and neurodegenerative diseases. Its dysregulation in certain cancers suggests a role in tumor progression and resistance to apoptosis. The connection to Bcl-2 highlights this aspect as both are involved in the suppression of cell death. In neurodegenerative diseases like Alzheimer's altered PAC-2 function might contribute to the pathological accumulation of damaged cells influenced by its effect on apoptotic pathways. Understanding these links could advance therapeutic strategies for related conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG1. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization.

See full target information PSMG2

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 21:2531-2549 PubMed40303289

2025

PSMG2 role in tumorigenesis and stemness mediated by protein accumulation, reticulum stress and autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Asunción Espinosa-Sánchez,Elena Blanco-Alcaina,Amancio Carnero

eLife 12: PubMed37470242

2023

Identification of candidate mitochondrial inheritance determinants using the mammalian cell-free system.

Applications

Unspecified application

Species

Unspecified reactive species

Dalen Zuidema,Alexis Jones,Won-Hee Song,Michal Zigo,Peter Sutovsky

Frontiers in immunology 14:1117545 PubMed36936958

2023

Nocardia rubra cell-wall skeleton activates an immune response in cervical tissue stimulating FPR3 to enhance dendritic cell-mediated Th1 differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Qianyu Guo,Wei Chen,Junyi Sun,Chunfang Zhao,Xue Bai,Yanan Zhang,Ke Liu,Lei Zhang,Suxia Shao

Cell reports. Medicine 3:100741 PubMed36099919

2022

PSMG2-controlled proteasome-autophagy balance mediates the tolerance for MEK-targeted therapy in triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xueyan Wang,Jing Yu,Xiaowei Liu,Dan Luo,Yanchu Li,Linlin Song,Xian Jiang,Xiaomeng Yin,Yan Wang,Li Chai,Ting Luo,Jing Jing,Hubing Shi

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PAC-2 antibody [EPR9947(2)]

Western blot - Anti-PAC-2 antibody [EPR9947(2)] (AB172909)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise