Rabbit Polyclonal PACAP-38 antibody. Suitable for IHC-P and reacts with Mouse, Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human ADCYAP1 aa 150 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
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Publications
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- International journal of environmental research and public health 19:2022Endocrine Disruptor Bisphenol a Affects the Neurochemical Profile of Nerve Fibers in the Aortic Arch Wall in the Domestic Pig.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiliana Rytel,László Könyves,Slawomir GonkowskiPubMed 35627499
- Translational psychiatry 10:3962020Early-life stress alters affective behaviors in adult mice through persistent activation of CRH-BDNF signaling in the oval bed nucleus of the stria terminalis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPu Hu et. al.PubMed 33177511
- The Journal of neuroscience : the official journal 40:2519-25372020Chronic Stress Induces Maladaptive Behaviors by Activating Corticotropin-Releasing Hormone Signaling in the Mouse Oval Bed Nucleus of the Stria Terminalis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPu Hu et. al.PubMed 32054675
- Frontiers in neuroscience 13:13262019Distinct VIP and PACAP Functions in the Distal Nerve Stump During Peripheral Nerve Regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPatricia K Woodley et. al.PubMed 31920495
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ADCYAP1 aa 150 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P18509
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/500.00000 | Notes When using a fluorescent probe the recommended dilution is 1/50 – 1/200. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/100.00000 - 1/500.00000 | Notes When using a fluorescent probe the recommended dilution is 1/50 – 1/200. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes When using a fluorescent probe the recommended dilution is 1/50 – 1/200. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Associated Products
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Target data
Function
Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells. Promotes neuron projection development through the RAPGEF2/Rap1/B-Raf/ERK pathway. In chromaffin cells, induces long-lasting increase of intracellular calcium concentrations and neuroendocrine secretion (By similarity). Involved in the control of glucose homeostasis, induces insulin secretion by pancreatic beta cells (By similarity).
Alternative names
Pituitary adenylate cyclase-activating polypeptide, PACAP, ADCYAP1
Recommended products
Rabbit Polyclonal PACAP-38 antibody. Suitable for IHC-P and reacts with Mouse, Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human ADCYAP1 aa 150 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ADCYAP1 aa 150 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P18509
- Purification technique
- Affinity purification Protein A
- Specificity
ab216627 is specific to the cleaved PACAP-38 chain.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Pituitary adenylate cyclase-activating polypeptide-38 (PACAP-38) is a neuropeptide with key roles in the central and peripheral nervous systems. Another name is vasoactive intestinal peptide-related peptide. PACAP-38 is a 38-amino acid peptide and its molecular weight is approximately 4.5 kDa. It expresses widely notably in the hypothalamus pituitary gland and adrenal medulla. PACAP-38 interacts with specific receptors such as PAC1 and VPAC receptors to mediate its functions.
- Biological function summary
PACAP-38 modulates various physiological processes through its signaling activities. It acts in neurotransmission neuroprotection and neurogenesis. In the immune system PACAP-38 contributes to anti-inflammatory responses by regulating cytokine production. It does not form part of a larger protein complex but exerts its effects independently via receptor binding serving important roles in maintaining homeostasis.
- Pathways
PACAP-38 influences several signaling cascades. It is integral to the cAMP-PKA pathway which is important for regulating a variety of cellular processes including hormonal secretion and metabolic activity. Additionally PACAP-38 is involved in the MAPK/ERK pathway contributing to cell proliferation and survival. Related proteins such as the PAC1 receptor and adenylate cyclase play roles alongside PACAP-38 in these pathways.
- Associated diseases and disorders
PACAP-38 connects to migraine and schizophrenia. PACAP-38's role in dilation of cerebral arteries and its modulation of pain pathways link it to migraine where levels of this peptide can dictate disease manifestation. In schizophrenia altered PACAP-38 signaling affects neural circuits contributing to the disorder's symptoms. Through these disorders PACAP-38 interacts with proteins like calcitonin gene-related peptide (CGRP) in migraines and dopamine receptors in psychiatric conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACAP-38 antibody (ab216627)
Immunohistochemical analysis of formalin-fixed paraffin-embedded rat brain tissue, labeling PACAP-38 using ab216627 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACAP-38 antibody (ab216627)
Immunohistochemical analysis of formalin-fixed paraffin-embedded mouse small intestine tissue, labeling PACAP-38 using ab216627 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACAP-38 antibody (ab216627)
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human kidney tissue, labeling PACAP-38 using ab216627 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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