Rabbit Recombinant Monoclonal PACAP antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.
Images
![Immunoprecipitation - Anti-PACAP antibody [EPR11543(B)] - BSA and Azide free (AB249570), expandable thumbnail](https://content.abcam.com/products/images/pacap-antibody-epr11543b-bsa-and-azide-free-ab249570--immunoprecipitation-img67378.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-PACAP antibody [EPR11543(B)] - BSA and Azide free (AB249570)")
![Western blot - Anti-PACAP antibody [EPR11543(B)] - BSA and Azide free (AB249570), expandable thumbnail](https://content.abcam.com/products/images/pacap-antibody-epr11543b-bsa-and-azide-free-ab249570--western-blot-img70397.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PACAP antibody [EPR11543(B)] - BSA and Azide free (AB249570)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | IHC | Flow Cyt | WB | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Associates with immunoglobulin M (IgM) heavy and light chains and promotes IgM assembly and secretion. May exert its effect by acting as a molecular chaperone or as an oxidoreductase as it displays a low level of oxidoreductase activity (By similarity). Isoform 2 may be involved in regulation of apoptosis. Helps to diversify peripheral B-cell functions by regulating Ca(2+) stores, antibody secretion and integrin activation.Acts as a hormone-regulated adipokine/pro-inflammatory cytokine that is implicated in causing chronic inflammation, affecting cellular expansion and blunting insulin response in adipocytes. May have a role in the onset of insulin resistance.
Alternative names
MEDA7, PACAP, HSPC190, MZB1, Marginal zone B- and B1-cell-specific protein, Mesenteric estrogen-dependent adipose 7, Plasma cell-induced resident endoplasmic reticulum protein, Proapoptotic caspase adapter protein, MEDA-7, Plasma cell-induced resident ER protein, pERp1
Recommended products
Rabbit Recombinant Monoclonal PACAP antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR11543(B)
- Purification technique
Affinity purification
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab249570 is the carrier-free version of Anti-PACAP antibody [EPR11543(B)] ab170891.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PACAP also known as Pituitary Adenylate Cyclase-Activating Polypeptide is a neuropeptide functioning as a neurotransmitter and neuromodulator. It belongs to the vasoactive intestinal peptide (VIP)/secretin/glucagon hormone family. PACAP consists of 38 amino acid residues and has a molecular mass of approximately 4.5 kDa. It is expressed in various regions including the central nervous system (CNS) endocrine cells and peripheral tissues. This broad expression pattern supports its diverse role in physiological and neurobiological processes.
- Biological function summary
PACAP exerts multiple effects by binding to three G protein-coupled receptors: PAC1 VPAC1 and VPAC2. It influences processes like neuroprotection neurodevelopment and neurotransmission. As part of the PACAP/VIP receptor complex it regulates cyclic AMP production and calcium signaling mediating its effects in both neural and non-neural tissues. It is particularly involved in paired functions with neurotransmitters and other signaling molecules indicating its key role in maintaining homeostasis.
- Pathways
The PACAP signaling mechanism integrates into the cAMP-dependent pathway and the MAPK/ERK pathway. These pathways are central to cell proliferation differentiation and survival. PACAP's interaction with MAPK/ERK pathway involves proteins such as Raf and MEK further modulating gene expression and synaptic plasticity. By this mechanism PACAP plays a vital role in modulating neurotransmission and neuroendocrine functions.
- Associated diseases and disorders
PACAP is linked to migraine and neurodegenerative diseases like Alzheimer's disease. Alterations in PACAP signaling can affect pain pathways and neuroinflammation potentially exacerbating such conditions. PACAP's interaction with proteins like CGRP (Calcitonin Gene-Related Peptide) is significant as both are implicated in the pathophysiology of migraines. Moreover in Alzheimer’s disease the dysregulation of PACAP and its receptors contributes to neuroinflammation and neurodegeneration.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunoprecipitation - Anti-PACAP antibody [EPR11543(B)] - BSA and Azide free (ab249570), expandable thumbnail](https://content.abcam.com/products/images/pacap-antibody-epr11543b-bsa-and-azide-free-ab249570--immunoprecipitation-img67378.jpg "Immunoprecipitation - Anti-PACAP antibody [EPR11543(B)] - BSA and Azide free (ab249570)")
Immunoprecipitation - Anti-PACAP antibody [EPR11543(B)] - BSA and Azide free (ab249570)
This data was developed using Anti-PACAP antibody [EPR11543(B)] ab170891, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from (1) Raji cell lysate or (2) 1X PBS (negative control) immunoprecipitated using Anti-PACAP antibody [EPR11543(B)] ab170891 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.
All lanes: Immunoprecipitation - Anti-PACAP antibody [EPR11543(B)] (Anti-PACAP antibody [EPR11543(B)] ab170891)
Predicted band size: 21 kDa
![Western blot - Anti-PACAP antibody [EPR11543(B)] - BSA and Azide free (ab249570), expandable thumbnail](https://content.abcam.com/products/images/pacap-antibody-epr11543b-bsa-and-azide-free-ab249570--western-blot-img70397.jpg "Western blot - Anti-PACAP antibody [EPR11543(B)] - BSA and Azide free (ab249570)")
Western blot - Anti-PACAP antibody [EPR11543(B)] - BSA and Azide free (ab249570)
This data was developed using Anti-PACAP antibody [EPR11543(B)] ab170891, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-PACAP antibody [EPR11543(B)] (Anti-PACAP antibody [EPR11543(B)] ab170891) at 1/10000 dilution
Lane 1: Daudi cell lysates at 10 µg
Lane 2: Ramos cell lysates at 10 µg
Lane 3: Raji cell lysates at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 21 kDa
Downloads
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