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Rabbit Recombinant Monoclonal PACAP antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 6 publications.

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Images

Western blot - Anti-PACAP antibody [EPR11544] (AB181205), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACAP antibody [EPR11544] (AB181205), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACAP antibody [EPR11544] (AB181205), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Mouse
Predicted
Predicted
Rat
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/10000 - 1/50000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Associates with immunoglobulin M (IgM) heavy and light chains and promotes IgM assembly and secretion. May exert its effect by acting as a molecular chaperone or as an oxidoreductase as it displays a low level of oxidoreductase activity (By similarity). Isoform 2 may be involved in regulation of apoptosis. Helps to diversify peripheral B-cell functions by regulating Ca(2+) stores, antibody secretion and integrin activation. Acts as a hormone-regulated adipokine/pro-inflammatory cytokine that is implicated in causing chronic inflammation, affecting cellular expansion and blunting insulin response in adipocytes. May have a role in the onset of insulin resistance.

Alternative names

MEDA7, PACAP, HSPC190, MZB1, Marginal zone B- and B1-cell-specific protein, Mesenteric estrogen-dependent adipose 7, Plasma cell-induced resident endoplasmic reticulum protein, Proapoptotic caspase adapter protein, MEDA-7, Plasma cell-induced resident ER protein, pERp1

Recommended products

Rabbit Recombinant Monoclonal PACAP antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 6 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR11544
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PACAP also known as Pituitary Adenylate Cyclase-Activating Polypeptide is a neuropeptide functioning as a neurotransmitter and neuromodulator. It belongs to the vasoactive intestinal peptide (VIP)/secretin/glucagon hormone family. PACAP consists of 38 amino acid residues and has a molecular mass of approximately 4.5 kDa. It is expressed in various regions including the central nervous system (CNS) endocrine cells and peripheral tissues. This broad expression pattern supports its diverse role in physiological and neurobiological processes.

Biological function summary

PACAP exerts multiple effects by binding to three G protein-coupled receptors: PAC1 VPAC1 and VPAC2. It influences processes like neuroprotection neurodevelopment and neurotransmission. As part of the PACAP/VIP receptor complex it regulates cyclic AMP production and calcium signaling mediating its effects in both neural and non-neural tissues. It is particularly involved in paired functions with neurotransmitters and other signaling molecules indicating its key role in maintaining homeostasis.

Pathways

The PACAP signaling mechanism integrates into the cAMP-dependent pathway and the MAPK/ERK pathway. These pathways are central to cell proliferation differentiation and survival. PACAP's interaction with MAPK/ERK pathway involves proteins such as Raf and MEK further modulating gene expression and synaptic plasticity. By this mechanism PACAP plays a vital role in modulating neurotransmission and neuroendocrine functions.

Associated diseases and disorders

PACAP is linked to migraine and neurodegenerative diseases like Alzheimer's disease. Alterations in PACAP signaling can affect pain pathways and neuroinflammation potentially exacerbating such conditions. PACAP's interaction with proteins like CGRP (Calcitonin Gene-Related Peptide) is significant as both are implicated in the pathophysiology of migraines. Moreover in Alzheimer’s disease the dysregulation of PACAP and its receptors contributes to neuroinflammation and neurodegeneration.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-PACAP antibody [EPR11544] (ab181205), expandable thumbnail

    Western blot - Anti-PACAP antibody [EPR11544] (ab181205)

    All lanes: Western blot - Anti-PACAP antibody [EPR11544] (ab181205) at 1/50000 dilution

    Lane 1: Raji cell lysate at 20 µg

    Lane 2: Ramos cell lysate at 20 µg

    Lane 3: MOLT-4 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACAP antibody [EPR11544] (ab181205), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACAP antibody [EPR11544] (ab181205)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PACAP with ab181205 at 1/250 dilution. The slide is counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACAP antibody [EPR11544] (ab181205), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACAP antibody [EPR11544] (ab181205)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PACAP with ab181205 at 1/250 dilution. The slide is counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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