AB183103

Anti-PACAP receptor/ADCYAP1R1 antibody

Name: Anti-PACAP receptor/ADCYAP1R1 antibody (ab183103) | Abcam
Brand: Abcam Limited
SKU: AB183103
Price: 535 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal PACAP receptor/ADCYAP1R1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human Pituitary adenylate cyclase-activating polypeptide type I receptor aa 1-50.

View Alternative Names

Pituitary adenylate cyclase-activating polypeptide type I receptor, PAC1 receptor, PAC1R, PACAP type I receptor, PACAP-R-1, PACAP-R1, ADCYAP1R1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACAP receptor/ADCYAP1R1 antibody (AB183103)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACAP receptor/ADCYAP1R1 antibody (AB183103)

Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling ADCYAP1R1 with ab183103 at 1/70 dilution. Image on the right is treated with the synthetic peptide.

Supplier Data

Western blot - Anti-PACAP receptor/ADCYAP1R1 antibody (AB183103)

10%SDS-PAGE

All lanes:

Western blot - Anti-PACAP receptor/ADCYAP1R1 antibody (ab183103) at 1/2000 dilution

Lane 1:

Hela cell lysate at 40 µg

Lane 2:

Mouse stomach tissue lysate at 40 µg

Lane 3:

Mouse brain tissue lysate at 40 µg

Secondary

All lanes:

Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution

Predicted band size: 53 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human Pituitary adenylate cyclase-activating polypeptide type I receptor aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P41586

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PACAP receptor also known as ADCYAP1R1 is a G-protein-coupled receptor that interacts with the neuropeptide PACAP (Pituitary Adenylate Cyclase-Activating Polypeptide). This receptor plays a role in transmitting extracellular signals into cellular responses. It is comprised of approximately 525 amino acids with a considerable molecular mass of around 59 kDa. The PACAP receptors are widely expressed in the central nervous system including regions like the hypothalamus hippocampus and cerebellum. It also appears in peripheral tissues such as the adrenal glands and testis.

Biological function summary

PACAP receptor plays significant roles in both neuronal and endocrine systems. It modulates neurotransmitter release and affects neurotrophic activities. The receptor often operates in a receptor-ligand complex with PACAP which enables it to participate in neuroprotective and neurite outgrowth functions. This receptor actively regulates activities within the hypothalamic-pituitary-adrenal axis impacting stress responses and circadian rhythms.

Pathways

Many processes involve the PACAP receptor through essential signaling pathways like the cAMP-PKA and MAPK pathways. These pathways integrate signals from multiple receptor types including glucocorticoid receptors. The PACAP receptor often interacts with other adenylyl cyclase-activating proteins enhancing its involvement in neural cAMP synthesis and related functions. This places the receptor within key pathways regulating brain development and neuroplasticity.

The PACAP receptor holds connections to both mental health and neurological conditions. Its abnormal function has associations with disorders such as anxiety-related disorders and migraine. The receptor's relationship with proteins like the CRH (Corticotropin-Releasing Hormone) receptor further implicates its role in the pathophysiology of stress-induced disorders. Targeting PACAP receptors can thereby offer therapeutic potential in the management of these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

G protein-coupled receptor activated by the neuropeptide pituitary adenylate cyclase-activating polypeptide (ADCYAP1/PACAP) (PubMed : 32047270, PubMed : 33715378, PubMed : 35477937, PubMed : 36385145). Binds both PACAP27 and PACAP38 bioactive peptides (PubMed : 32047270, PubMed : 35477937, PubMed : 36385145). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. Activates cAMP-dependent pathway (PubMed : 32047270, PubMed : 33715378, PubMed : 35477937, PubMed : 36385145). May regulate the release of adrenocorticotropin, luteinizing hormone, growth hormone, prolactin, epinephrine, and catecholamine. May play a role in spermatogenesis and sperm motility. Causes smooth muscle relaxation and secretion in the gastrointestinal tract (PubMed : 32047270, PubMed : 33715378).

See full target information Pituitary adenylate cyclase-activating polypeptide type I receptor

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 21:261 PubMed37553718

2023

Exosome-sheathed ROS-responsive nanogel to improve targeted therapy in perimenopausal depression.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Hu,Min Zhao,Hui Wang,Yang Guo,Xiaolan Cheng,Tong Zhao,Hanqing Wang,Yafeng Zhang,Yong Ma,Weiwei Tao

iScience 26:106652 PubMed37182096

2023

Protein quality control machinery supports primary ciliogenesis by eliminating GDP-bound Rab8-family GTPases.

Applications

Unspecified application

Species

Unspecified reactive species

Toshiki Takahashi,Jun Shirai,Miyo Matsuda,Sae Nakanaga,Shin Matsushita,Kei Wakita,Mizuki Hayashishita,Rigel Suzuki,Aya Noguchi,Naoto Yokota,Hiroyuki Kawahara

The Journal of general physiology 155: PubMed36538657

2022

PACAP and acetylcholine cause distinct Ca2+ signals and secretory responses in chromaffin cells.

Applications

Unspecified application

Species

Unspecified reactive species

Alina Morales,Ramkumar Mohan,Xiaohuan Chen,Breanna L Coffman,Mounir Bendahmane,Lester Watch,Joshua L West,Shreeya Bakshi,John R Traynor,David R Giovannucci,Paul J Kammermeier,Daniel Axelrod,Kevin P M Currie,Alan V Smrcka,Arun Anantharam

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-PACAP receptor/ADCYAP1R1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACAP receptor/ADCYAP1R1 antibody (AB183103)

Western blot - Anti-PACAP receptor/ADCYAP1R1 antibody (AB183103)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise