AB216664

Anti-PACAP receptor/ADCYAP1R1 antibody

Name: Anti-PACAP receptor/ADCYAP1R1 antibody (ab216664) | Abcam
Brand: Abcam Limited
SKU: AB216664

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(1 Publication)

Rabbit Polyclonal PACAP receptor/ADCYAP1R1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Mouse Pituitary adenylate cyclase-activating polypeptide type I receptor aa 250-300 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Pituitary adenylate cyclase-activating polypeptide type I receptor, PACAP type I receptor, PACAP-R-1, PACAP-R1

2 Images

Supplier Data

Western blot - Anti-PACAP receptor/ADCYAP1R1 antibody (AB216664)

12% gel

All lanes:

Western blot - Anti-PACAP receptor/ADCYAP1R1 antibody (ab216664) at 1/200 dilution

Lane 1:

Rat brain lysate

Lane 2:

Rat heart lysate

Secondary

All lanes:

Goat anti-rabbit IgG (H+L), HRP-conjugated at 1/3000 dilution

Predicted band size: 53 kDa

Observed band size: 58 kDa

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Supplier Data

Western blot - Anti-PACAP receptor/ADCYAP1R1 antibody (AB216664)

All lanes:

Western blot - Anti-PACAP receptor/ADCYAP1R1 antibody (ab216664) at 1/300 dilution

Lane 1:

Mouse brain lysate

Lane 2:

A549 cell lysate

Predicted band size: 46 kDa,53 kDa,54 kDa,58 kDa,67 kDa

Observed band size: 55 kDa,61 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

Synthetic Peptide within Mouse Pituitary adenylate cyclase-activating polypeptide type I receptor aa 250-300 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P70205

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PACAP receptor also known as ADCYAP1R1 is a G-protein-coupled receptor that interacts with the neuropeptide PACAP (Pituitary Adenylate Cyclase-Activating Polypeptide). This receptor plays a role in transmitting extracellular signals into cellular responses. It is comprised of approximately 525 amino acids with a considerable molecular mass of around 59 kDa. The PACAP receptors are widely expressed in the central nervous system including regions like the hypothalamus hippocampus and cerebellum. It also appears in peripheral tissues such as the adrenal glands and testis.

Biological function summary

PACAP receptor plays significant roles in both neuronal and endocrine systems. It modulates neurotransmitter release and affects neurotrophic activities. The receptor often operates in a receptor-ligand complex with PACAP which enables it to participate in neuroprotective and neurite outgrowth functions. This receptor actively regulates activities within the hypothalamic-pituitary-adrenal axis impacting stress responses and circadian rhythms.

Pathways

Many processes involve the PACAP receptor through essential signaling pathways like the cAMP-PKA and MAPK pathways. These pathways integrate signals from multiple receptor types including glucocorticoid receptors. The PACAP receptor often interacts with other adenylyl cyclase-activating proteins enhancing its involvement in neural cAMP synthesis and related functions. This places the receptor within key pathways regulating brain development and neuroplasticity.

The PACAP receptor holds connections to both mental health and neurological conditions. Its abnormal function has associations with disorders such as anxiety-related disorders and migraine. The receptor's relationship with proteins like the CRH (Corticotropin-Releasing Hormone) receptor further implicates its role in the pathophysiology of stress-induced disorders. Targeting PACAP receptors can thereby offer therapeutic potential in the management of these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This is a receptor for PACAP-27 and PACAP-38. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. May regulate the release of adrenocorticotropin, luteinizing hormone, growth hormone, prolactin, epinephrine, and catecholamine. May play a role in spermatogenesis and sperm motility. Causes smooth muscle relaxation and secretion in the gastrointestinal tract.

See full target information Pituitary adenylate cyclase-activating polypeptide type I receptor

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 24:726 PubMed36340606

2022

ASPP2 promotes cell apoptosis in cervical cancer through inhibiting autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Feiyun Jiang,Ganxia Bian,Jiehua Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PACAP receptor/ADCYAP1R1 antibody

Western blot - Anti-PACAP receptor/ADCYAP1R1 antibody (AB216664)

Western blot - Anti-PACAP receptor/ADCYAP1R1 antibody (AB216664)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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