AB137390

Anti-PACSIN1 antibody

Name: Anti-PACSIN1 antibody (ab137390) | Abcam
Brand: Abcam Limited
SKU: AB137390
Price: 520 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal PACSIN1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PACSIN1.

View Alternative Names

KIAA1379, PACSIN1, Protein kinase C and casein kinase substrate in neurons protein 1, Syndapin-1

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-PACSIN1 antibody (AB137390)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PACSIN1 antibody (AB137390)

Immunofluorescence analysis of DIV14 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min labelling PACSIN1 protein using ab137390 at a 1/500 dilution (Green).
beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody at a 1/500 dilution (Red). DAPI was used for nuclear staining (Blue).

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Western blot - Anti-PACSIN1 antibody (AB137390)

7.5% SDS PAGE

All lanes:

Western blot - Anti-PACSIN1 antibody (ab137390) at 1/500 dilution

All lanes:

MOLT4 whole cell lysate at 30 µg

Predicted band size: 51 kDa

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Supplier Data

Western blot - Anti-PACSIN1 antibody (AB137390)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-PACSIN1 antibody (ab137390) at 1/10000 dilution

Lane 1:

Mouse brain tissue lysate at 50 µg

Lane 2:

Rat brain tissue lysates at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 51 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PACSIN1.

Q9BY11

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PACSIN1 also known as Syndapin I is a protein that plays a role in membrane trafficking and cytoskeleton organization. It has an alternative name Syndapin I and its molecular mass is approximately 50 kDa. This protein is expressed in the brain particularly in the neuronal tissues where it associates with membranes and interacts with other proteins to regulate endocytosis. Through its F-BAR domain PACSIN1 senses membrane curvature and participates in membrane tubulation processes.

Biological function summary

PACSIN1 contributes to neuronal function by being a component of endocytic and cytoskeletal complexes. It interacts with dynamin and synaptojanin-1 facilitating the assembly of these complexes upon curved membranes. This interaction promotes synaptic vesicle recycling which is critical for efficient neurotransmitter release and synaptic transmission. PACSIN1 is an important part of the vesicle trafficking machinery in neurons directly affecting the dynamics of synapses and overall neuronal communication.

Pathways

PACSIN1 is integrated into the synaptic vesicle cycle and the actin cytoskeleton remodeling pathway. It collaborates with proteins like dynamin I and actin to regulate endocytosis and membrane shaping during synaptic vesicle recycling. These pathways underpin the synaptic vesicle cycle's efficiency and the maintenance of the actin cytoskeleton structure. PACSIN1's interaction with dynamin I demonstrates its significant role in modulating endocytosis speed and efficacy.

PACSIN1 has associations with neural complications like epilepsy and Parkinson's disease. It interacts with synaptic proteins such as synaptojanin-1 whose dysregulation can lead to synaptic dysfunction contributing to these disorders. Impaired PACSIN1 function could disrupt normal synaptic activity leading to neurodegenerative symptoms observed in these diseases. Understanding its role can provide insights into potential therapeutic targets for neurological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Plays a role in the reorganization of the microtubule cytoskeleton via its interaction with MAPT; this decreases microtubule stability and inhibits MAPT-induced microtubule polymerization. Plays a role in cellular transport processes by recruiting DNM1, DNM2 and DNM3 to membranes. Plays a role in the reorganization of the actin cytoskeleton and in neuron morphogenesis via its interaction with COBL and WASL, and by recruiting COBL to the cell cortex. Plays a role in the regulation of neurite formation, neurite branching and the regulation of neurite length. Required for normal synaptic vesicle endocytosis; this process retrieves previously released neurotransmitters to accommodate multiple cycles of neurotransmission. Required for normal excitatory and inhibitory synaptic transmission (By similarity). Binds to membranes via its F-BAR domain and mediates membrane tubulation.

See full target information PACSIN1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:5285 PubMed37648685

2023

Reversal of cell, circuit and seizure phenotypes in a mouse model of DNM1 epileptic encephalopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine Bonnycastle,Katharine L Dobson,Eva-Maria Blumrich,Akshada Gajbhiye,Elizabeth C Davenport,Marie Pronot,Moritz Steinruecke,Matthias Trost,Alfredo Gonzalez-Sulser,Michael A Cousin

Neuron 110:2815-2835.e13 PubMed35809574

2022

Dynamin is primed at endocytic sites for ultrafast endocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuuta Imoto,Sumana Raychaudhuri,Ye Ma,Pascal Fenske,Eduardo Sandoval,Kie Itoh,Eva-Maria Blumrich,Hideaki T Matsubayashi,Lauren Mamer,Fereshteh Zarebidaki,Berit Söhl-Kielczynski,Thorsten Trimbuch,Shraddha Nayak,Janet H Iwasa,Jian Liu,Bin Wu,Taekjip Ha,Takanari Inoue,Erik M Jorgensen,Michael A Cousin,Christian Rosenmund,Shigeki Watanabe

Cell and tissue research 384:613-624 PubMed33471197

2021

Endoglin and TGF-β signaling in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Isabel Burghardt,Elisa Ventura,Tobias Weiss,Judith Johanna Schroeder,Katharina Seystahl,Christian Zielasek,Dorothee Gramatzki,Michael Weller

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-PACSIN1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-PACSIN1 antibody (AB137390)

Western blot - Anti-PACSIN1 antibody (AB137390)

Western blot - Anti-PACSIN1 antibody (AB137390)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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