Anti-PACSIN1 antibody [EPR29589-587] (ab323573)

Rabbit Recombinant Monoclonal PACSIN1 antibody. Suitable for WB, IHC-P and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.

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Images

Western blot - Anti-PACSIN1 antibody [EPR29589-587] (AB323573), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	IP	ICC/IF
Human	Tested	Tested	Not recommended	Not recommended
Mouse	Tested	Tested	Not recommended	Not recommended
Rat	Tested	Tested	Not recommended	Not recommended
Recombinant fragment - Human	Tested	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Recombinant fragment - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Recombinant fragment - Human	Dilution info -	Notes -

Target data

See full target information PACSIN1

Function

Plays a role in the reorganization of the microtubule cytoskeleton via its interaction with MAPT; this decreases microtubule stability and inhibits MAPT-induced microtubule polymerization. Plays a role in cellular transport processes by recruiting DNM1, DNM2 and DNM3 to membranes. Plays a role in the reorganization of the actin cytoskeleton and in neuron morphogenesis via its interaction with COBL and WASL, and by recruiting COBL to the cell cortex. Plays a role in the regulation of neurite formation, neurite branching and the regulation of neurite length. Required for normal synaptic vesicle endocytosis; this process retrieves previously released neurotransmitters to accommodate multiple cycles of neurotransmission. Required for normal excitatory and inhibitory synaptic transmission (By similarity). Binds to membranes via its F-BAR domain and mediates membrane tubulation.

Alternative names

KIAA1379, PACSIN1, Protein kinase C and casein kinase substrate in neurons protein 1, Syndapin-1

Rabbit Recombinant Monoclonal PACSIN1 antibody. Suitable for WB, IHC-P and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR29589-587
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Western blot - Anti-PACSIN1 antibody [EPR29589-587] (ab323573)
PACSIN1 Western blot staining using rabbit Anti-PACSIN1 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: Human liver, mouse and rat lung.
Dimerization of two identical 51 kDa subunits of PACSIN1 results in the formation of the approximately 100 kDa band (PMID: 27488904).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-PACSIN1 antibody [EPR29589-587] (ab323573) at 1/1000 dilution
Lane 1: Human hippocampus tissue lysate at 20 µg
Lane 2: Human cerebellum tissue lysate at 20 µg
Lane 3: Human liver tissue lysate at 20 µg
Lane 4: Mouse hippocampus tissue lysate at 20 µg
Lane 5: Mouse cerebellum tissue lysate at 20 µg
Lane 6: Mouse lung tissue lysate at 20 µg
Lane 7: Rat hippocampus tissue lysate at 20 µg
Lane 8: Rat cerebellum tissue lysate at 20 µg
Lane 9: Rat lung tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 51 kDa, 36 kDa
Exposure time: 180s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACSIN1 antibody [EPR29589-587] (ab323573)
PACSIN1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Rat cerebrum using rabbit Anti-PACSIN1 antibody
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling PACSIN1 with ab323573 at 1/5000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat cerebrum. The section was incubated with ab323573 for 10 mins at room temperature.

Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACSIN1 antibody [EPR29589-587] (ab323573)
PACSIN1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human gastric cancer using rabbit Anti-PACSIN1 antibody
Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue labeling PACSIN1 with ab323573 at 1/2000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on immune cells of human gastric cancer (PMID: 38826133). The section was incubated with ab323573 for 10 mins at room temperature.

Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACSIN1 antibody [EPR29589-587] (ab323573)
PACSIN1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse lung using rabbit Anti-PACSIN1 antibody
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling PACSIN1 with ab323573 at 1/5000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse lung (PMID: 35534740). The section was incubated with ab323573 for 10 mins at room temperature.

Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACSIN1 antibody [EPR29589-587] (ab323573)
PACSIN1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Rat lung using rabbit Anti-PACSIN1 antibody
Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling PACSIN1 with ab323573 at 1/5000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on rat lung. The section was incubated with ab323573 for 10 mins at room temperature.

Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACSIN1 antibody [EPR29589-587] (ab323573)
PACSIN1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human liver using rabbit Anti-PACSIN1 antibody
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling PACSIN1 with ab323573 at 1/2000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human liver. The section was incubated with ab323573 for 10 mins at room temperature.

Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACSIN1 antibody [EPR29589-587] (ab323573)
PACSIN1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human cerebrum using rabbit Anti-PACSIN1 antibody
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling PACSIN1 with ab323573 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human cerebrum. The section was incubated with ab323573 for 10 mins at room temperature.

Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Western blot - Anti-PACSIN1 antibody [EPR29589-587] (ab323573)
PACSIN1 Western blot staining using rabbit Anti-PACSIN1 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody does not cross-react with recombinant human PACSIN2 and PACSIN3 by Western Blot.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-PACSIN1 antibody [EPR29589-587] (ab323573) at 1/1000 dilution
Lane 1: No-tagged human PACSIN1 recombinant protein at 20 ng
Lane 2: His-tagged human PACSIN2 recombinant protein at 20 ng
Lane 3: His-tagged human PACSIN3 recombinant protein at 20 ng
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 51 kDa, 50-55 kDa
Exposure time: 180s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACSIN1 antibody [EPR29589-587] (ab323573)
PACSIN1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse cerebrum using rabbit Anti-PACSIN1 antibody
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling PACSIN1 with ab323573 at 1/5000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse cerebrum (PMID: 35534740). The section was incubated with ab323573 for 10 mins at room temperature.

Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Western blot - Anti-PACSIN1 antibody [EPR29589-587] (ab323573)
PACSIN1 Western blot staining using rabbit Anti-PACSIN1 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: HeLa, 293T, 4T1.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-PACSIN1 antibody [EPR29589-587] (ab323573) at 1/1000 dilution
Lane 1: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg
Lane 2: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 4: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg
Lane 5: 4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 51 kDa, 36 kDa
Exposure time: 180s