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AB228589

Anti-PACSIN2 antibody

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(1 Review)

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(1 Publication)

Rabbit Polyclonal PACSIN2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Protein kinase C and casein kinase substrate in neurons protein 2.

View Alternative Names

Protein kinase C and casein kinase substrate in neurons protein 2, Syndapin-2, Syndapin-II, SdpII, PACSIN2

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACSIN2 antibody (AB228589)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACSIN2 antibody (AB228589)

Paraffin-embedded human colon carcinoma tissue stained for PACSIN2 with ab228589 at 1/500 dilution in immunohistochemical analysis

Western blot - Anti-PACSIN2 antibody (AB228589)
  • WB

Supplier Data

Western blot - Anti-PACSIN2 antibody (AB228589)

10% SDS-PAGE

All lanes:

Western blot - Anti-PACSIN2 antibody (ab228589) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 56 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human Protein kinase C and casein kinase substrate in neurons protein 2. The exact immunogen used to generate this antibody is proprietary information.

Q9UNF0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PACSIN2 also known as Protein kinase C and casein kinase substrate in neurons protein 2 is a protein encoded by the PACSIN2 gene. This protein has a mass of approximately 54 kDa and functions primarily in the cytoplasm. PACSIN2 is expressed in various tissues with notable expression in the brain and kidney. This protein plays a role in skeletal muscle regulation by interacting with actin and promoting changes in cell shape. PACSIN2 also contributes to the process of endocytosis particularly in coordinating the assembly of proteins that form vesicles.
Biological function summary

PACSIN2 regulates cytoskeletal dynamics and membrane trafficking. It acts as a part of a protein complex that includes dynamin which is essential for the fission of vesicular membranes from the plasma membrane during endocytosis. Through its involvement in these processes PACSIN2 assists in maintaining cellular morphology and transport mechanisms within cells. Its interaction with dynamin and actin indicates its role in modulating cellular infrastructure during response to environmental changes or cellular signals.

Pathways

PACSIN2 participates in the endocytic pathway and the regulation of cytoskeletal dynamics. It interacts with proteins like dynamin important in vesicle scission during endocytosis. Moreover PACSIN2 influences pathways related to actin dynamics and cellular morphology changes interacting with actin to coordinate cytoskeletal rearrangements. This involvement highlights its function in cellular adaptation to different stimuli.

PACSIN2 is associated with neurodegenerative diseases and cancer. Research has shown its potential involvement in Alzheimer's disease due to its role in membrane trafficking and signaling pathways. In the context of cancer its expression may correlate with abnormal cell proliferation and tumor progression. The interaction between PACSIN2 and dynamin further links it to these disorders as alterations in endocytosis and signaling can affect disease progression and cell growth.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulates the morphogenesis and endocytosis of caveolae (By similarity). Lipid-binding protein that is able to promote the tubulation of the phosphatidic acid-containing membranes it preferentially binds. Plays a role in intracellular vesicle-mediated transport. Involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization in the absence of EGF stimulus (PubMed : 21693584, PubMed : 23129763, PubMed : 23236520, PubMed : 23596323). Essential for endothelial organization in sprouting angiogenesis, modulates CDH5-based junctions. Facilitates endothelial front-rear polarity during migration by recruiting EHD4 and MICALL1 to asymmetric adherens junctions between leader and follower cells (By similarity).. (Microbial infection) Specifically enhances the efficiency of HIV-1 virion spread by cell-to-cell transfer (PubMed : 29891700). Also promotes the protrusion engulfment during cell-to-cell spread of bacterial pathogens like Listeria monocytogenes (PubMed : 31242077). Involved in lipid droplet formation, which is important for HCV virion assembly (PubMed : 31801866).
See full target information Protein kinase C and casein kinase substrate in neurons protein 2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Physiological reports 11:e15837 PubMed37813559

2023

In utero, lactational, or peripartal fluoxetine administration has differential implications on the murine maternal skeleton.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah P Fricke,Chandler J Krajco,Molly J Perry,Maggie A Reisner,Lauren J Brettingen,Lella A Wake,Julia F Charles,Laura L Hernandez
View all publications
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Product promise

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