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AB235095

Anti-PACSIN3 antibody

4

(1 Review)

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(1 Publication)

Rabbit Polyclonal PACSIN3 antibody. Suitable for WB, IHC-P and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Protein kinase C and casein kinase substrate in neurons protein 3 aa 1-300.

View Alternative Names

Protein kinase C and casein kinase substrate in neurons protein 3, SH3 domain-containing protein 6511, PACSIN3

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACSIN3 antibody (AB235095)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACSIN3 antibody (AB235095)

Paraffin-embedded human breast cancer tissue stained for PACSIN3 using ab235095 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACSIN3 antibody (AB235095)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACSIN3 antibody (AB235095)

Paraffin-embedded human adrenal gland tissue stained for PACSIN3 using ab235095 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-PACSIN3 antibody (AB235095)
  • WB

Supplier Data

Western blot - Anti-PACSIN3 antibody (AB235095)

All lanes:

Western blot - Anti-PACSIN3 antibody (ab235095) at 1/500 dilution

All lanes:

Rat liver tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 48 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human Protein kinase C and casein kinase substrate in neurons protein 3 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q9UKS6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PACSIN3 also known as Protein Kinase C and Casein Kinase Substrate in Neurons Protein 3 plays a role in cellular endocytosis and cytoskeleton organization. This protein is about 47 kDa in size. You find PACSIN3 widely expressed in muscle and heart tissues. It contains an F-BAR domain allowing it to bind membranes and promote membrane curvature. This F-BAR domain is a feature of proteins involved in membrane dynamics.
Biological function summary

The role of PACSIN3 extends into various cellular processes related to membrane trafficking where it acts to modulate vesicular transport and actin reorganization. PACSIN3 interacts with other proteins to form complexes that facilitate the internalization and recycling of receptors on the cell surface. Additionally its interaction with other proteins helps regulate cytoskeletal rearrangements essential for various cellular functions.

Pathways

Functions involving PACSIN3 integrate into key signaling pathways such as the endocytic pathway and the actin polymerization pathway. Within these pathways PACSIN3 interacts with proteins like dynamin a GTPase involved in the scission of budding vesicles from the membrane. Additionally it shares functional connections with proteins such as CTTN (cortactin) another actin-binding protein that influences actin polymerization and cellular movement processes.

Several links exist between PACSIN3 and diseases associated with dysregulated endocytosis and impaired cell signaling including muscular dystrophy and heart failure. PACSIN3's relationship with dynamin which is implicated in these conditions illustrates its potential role in the progression or mitigation of these disorders. Connections between PACSIN3 and other signaling proteins such as Rho GTPases further highlight its possible involvement in these pathological states given their importance in actin cytoskeleton dynamics and membrane trafficking.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in endocytosis and regulates internalization of plasma membrane proteins. Overexpression impairs internalization of SLC2A1/GLUT1 and TRPV4 and increases the levels of SLC2A1/GLUT1 and TRPV4 at the cell membrane. Inhibits the TRPV4 calcium channel activity (By similarity).
See full target information Protein kinase C and casein kinase substrate in neurons protein 3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancers 16: PubMed38611046

2024

FXR Agonism with Bile Acid Mimetic Reduces Pre-Clinical Triple-Negative Breast Cancer Burden.

Applications

Unspecified application

Species

Unspecified reactive species

Sydney C Joseph,Samson Eugin Simon,Margaret S Bohm,Minjeong Kim,Madeline E Pye,Boston W Simmons,Dillon G Graves,Stacey M Thomas-Gooch,Ubaid A Tanveer,Jeremiah R Holt,Suriyan Ponnusamy,Laura M Sipe,D Neil Hayes,Katherine L Cook,Ramesh Narayanan,Joseph F Pierre,Liza Makowski
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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