Rabbit Polyclonal PACSIN3 antibody. Suitable for WB, IHC-P and reacts with Rat, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Protein kinase C and casein kinase substrate in neurons protein 3 aa 1-300.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Protein kinase C and casein kinase substrate in neurons protein 3 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UKS6
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Predicted | Predicted |
| Rat | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Plays a role in endocytosis and regulates internalization of plasma membrane proteins. Overexpression impairs internalization of SLC2A1/GLUT1 and TRPV4 and increases the levels of SLC2A1/GLUT1 and TRPV4 at the cell membrane. Inhibits the TRPV4 calcium channel activity (By similarity).
Alternative names
Protein kinase C and casein kinase substrate in neurons protein 3, SH3 domain-containing protein 6511, PACSIN3
Recommended products
Rabbit Polyclonal PACSIN3 antibody. Suitable for WB, IHC-P and reacts with Rat, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Protein kinase C and casein kinase substrate in neurons protein 3 aa 1-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Protein kinase C and casein kinase substrate in neurons protein 3 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UKS6
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PACSIN3 also known as Protein Kinase C and Casein Kinase Substrate in Neurons Protein 3 plays a role in cellular endocytosis and cytoskeleton organization. This protein is about 47 kDa in size. You find PACSIN3 widely expressed in muscle and heart tissues. It contains an F-BAR domain allowing it to bind membranes and promote membrane curvature. This F-BAR domain is a feature of proteins involved in membrane dynamics.
- Biological function summary
The role of PACSIN3 extends into various cellular processes related to membrane trafficking where it acts to modulate vesicular transport and actin reorganization. PACSIN3 interacts with other proteins to form complexes that facilitate the internalization and recycling of receptors on the cell surface. Additionally its interaction with other proteins helps regulate cytoskeletal rearrangements essential for various cellular functions.
- Pathways
Functions involving PACSIN3 integrate into key signaling pathways such as the endocytic pathway and the actin polymerization pathway. Within these pathways PACSIN3 interacts with proteins like dynamin a GTPase involved in the scission of budding vesicles from the membrane. Additionally it shares functional connections with proteins such as CTTN (cortactin) another actin-binding protein that influences actin polymerization and cellular movement processes.
- Associated diseases and disorders
Several links exist between PACSIN3 and diseases associated with dysregulated endocytosis and impaired cell signaling including muscular dystrophy and heart failure. PACSIN3's relationship with dynamin which is implicated in these conditions illustrates its potential role in the progression or mitigation of these disorders. Connections between PACSIN3 and other signaling proteins such as Rho GTPases further highlight its possible involvement in these pathological states given their importance in actin cytoskeleton dynamics and membrane trafficking.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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3 product images
Western blot - Anti-PACSIN3 antibody (ab235095)
All lanes: Western blot - Anti-PACSIN3 antibody (ab235095) at 1/500 dilution
All lanes: Rat liver tissue lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 48 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACSIN3 antibody (ab235095)
Paraffin-embedded human breast cancer tissue stained for PACSIN3 using ab235095 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACSIN3 antibody (ab235095)
Paraffin-embedded human adrenal gland tissue stained for PACSIN3 using ab235095 at 1/100 dilution in immunohistochemical analysis.
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