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AB326703

Anti-PACT (PKR activating protein) / PRKRA antibody [SR4053]

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Rabbit Recombinant Monoclonal PACT (PKR activating protein) / PRKRA antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples.

View Alternative Names

PACT, RAX, HSD-14, HSD14, PRKRA, Interferon-inducible double-stranded RNA-dependent protein kinase activator A, PKR-associated protein X, PKR-associating protein X, Protein activator of the interferon-induced protein kinase

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACT (PKR activating protein) / PRKRA antibody [SR4053] (AB326703)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACT (PKR activating protein) / PRKRA antibody [SR4053] (AB326703)

Formalin-fixed, paraffin-embedded human colon cancer tissue stained for PACT (PKR activating protein) / PRKRA using antibody at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-PACT (PKR activating protein) / PRKRA antibody [SR4053] (AB326703)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PACT (PKR activating protein) / PRKRA antibody [SR4053] (AB326703)

ICC/IF staining of PACT (PKR activating protein) / PRKRA in 293T cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with antibody at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

Western blot - Anti-PACT (PKR activating protein) / PRKRA antibody [SR4053] (AB326703)
  • WB

Supplier Data

Western blot - Anti-PACT (PKR activating protein) / PRKRA antibody [SR4053] (AB326703)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : HepG2 whole cell lysates
Lane 2 : Mouse testis lysates
Lane 3 : Rat testis lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size : 34 kDa
Observed band size : 34 kDa

Exposure time : 5 seconds

All lanes:

Western blot - Anti-PACT (PKR activating protein) / PRKRA antibody [SR4053] (ab326703) at 1/1000 dilution

Lane 1:

HepG2 whole cell lysates at 20 µg

Lane 2:

Mouse testis lysates at 20 µg

Lane 3:

Rat testis lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

Exposure time: 5s

Western blot - Anti-PACT (PKR activating protein) / PRKRA antibody [SR4053] (AB326703)
  • WB

Supplier Data

Western blot - Anti-PACT (PKR activating protein) / PRKRA antibody [SR4053] (AB326703)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 : PRKRA Rabbit mAb knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.

All lanes:

Western blot - Anti-PACT (PKR activating protein) / PRKRA antibody [SR4053] (ab326703) at 1/1000 dilution

Lane 1:

Wild-type Hela cell lysate at 20 µg

Lane 2:

PRKRA Rabbit mAb knockdown Hela cell lysate at 20 µg

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SR4053

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner (By similarity). May function as regulator of gastric epithelial differentiation (By similarity).
See full target information PRKRA
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com