Rabbit Polyclonal PADI1 / PAD1 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PADI1.
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Publications
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- PLoS pathogens 19:e10118492023Citrullination profile analysis reveals peptidylarginine deaminase 3 as an HSV-1 target to dampen the activity of candidate antiviral restriction factors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSelina Pasquero,Francesca Gugliesi,Matteo Biolatti,Valentina Dell'Oste,Camilla Albano,Greta Bajetto,Gloria Griffante,Linda Trifirò,Bianca Brugo,Stefano Raviola,Davide Lacarbonara,Qiao Yang,Sen Sudeshna,Leonard Barasa,Hafeez Haniff,Paul R Thompson,Santo Landolfo,Marco De AndreaPubMed 38055760
- Antiviral research 200:1052782022Novel antiviral activity of PAD inhibitors against human beta-coronaviruses HCoV-OC43 and SARS-CoV-2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSelina Pasquero,Francesca Gugliesi,Gloria Griffante,Valentina Dell'Oste,Matteo Biolatti,Camilla Albano,Greta Bajetto,Serena Delbue,Lucia Signorini,Maria Dolci,Santo Landolfo,Marco De AndreaPubMed 35288208
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.8
Constituents: 50% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human PADI1. The exact immunogen used to generate this antibody is proprietary information. Database link Q9ULC6
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
Associated Products
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Target data
Function
Catalyzes the deimination of arginine residues of proteins.
Alternative names
PAD1, PDI1, PADI1, Protein-arginine deiminase type-1, Peptidylarginine deiminase I, Protein-arginine deiminase type I
Recommended products
Rabbit Polyclonal PADI1 / PAD1 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PADI1.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.8
Constituents: 50% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human PADI1. The exact immunogen used to generate this antibody is proprietary information. Database link Q9ULC6
- Purification technique
Affinity purification Immunogen
- Specificity
Cross reactivity with other PAD isoforms has not yet been tested
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PADI1 also known as PAD1 or PADIone is a protein of approximately 74 kDa that belongs to the peptidylarginine deiminase family. It catalyzes the conversion of arginine residues into citrulline in a calcium-dependent manner a process known as citrullination or deimination. The PADI1 expression is mainly in the epidermis hair follicles and other keratinizing tissues. The enzyme requires at least one Ca2+ ion for its activity and often associates with the nucleus and cytoplasm within cells.
- Biological function summary
PADI1 plays an important role in modulating structural changes in proteins involved in skin differentiation and hair formation. It is not known to form part of a larger protein complex but instead acts as a standalone enzyme in multiple cellular processes. The citrullination activity of PADI1 significantly affects the structural proteins like keratin and filaggrin influencing keratinocyte terminal differentiation and barrier function of the skin.
- Pathways
PADI1 is instrumental in the formation of the epidermal barrier by contributing to the terminal differentiation pathway of keratinocytes. This pathway ensures the proper development and maintenance of the skin layers. It associates with proteins such as keratin and filaggrin during this process. Furthermore PADI1 also links to pathways involved in the regulation of intracellular protein modification impacting the overall protein structure and function in tissues.
- Associated diseases and disorders
PADI1 has been linked to psoriasis and skin cancers. In psoriasis the aberrant expression or regulation of PADI1 can lead to improper protein citrullination affecting skin barrier function and immune responses. Moreover dysregulated activity of PADI1 connects to skin cancer progression through its impact on keratinocyte proliferation and differentiation. Research has shown interactions with other proteins like keratin as part of these disease processes relevant to its role in skin pathology.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-PADI1 / PAD1 antibody (ab181791)
All lanes: Western blot - Anti-PADI1 / PAD1 antibody (ab181791) at 1/20 dilution
All lanes: Human epidermis cell lysate
Predicted band size: 75 kDa
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