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AB321828

Anti-PADI2 / PAD2 antibody [EPR26940-342] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal PADI2 / PAD2 antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse samples.

View Alternative Names

KIAA0994, PAD2, PDI2, PADI2, Protein-arginine deiminase type-2, PAD-H19, Peptidylarginine deiminase II, Protein-arginine deiminase type II

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Sandwich ELISA - Anti-PADI2 / PAD2 antibody [EPR26940-342] - BSA and Azide free (Detector) (AB321828)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-PADI2 / PAD2 antibody [EPR26940-342] - BSA and Azide free (Detector) (AB321828)

Interpolated concentration of native PADI2 / PAD2 was measured at different sample concentrations. Undiluted samples are as follows : human serum 100%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in antibody diluent CP (ab282211). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-PADI2 / PAD2 antibody [EPR26940-342] - BSA and Azide free (Detector) (AB321828)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-PADI2 / PAD2 antibody [EPR26940-342] - BSA and Azide free (Detector) (AB321828)

Interpolated concentration of native PADI2 / PAD2 was measured at different sample concentrations. Undiluted samples are as follows : Mouse brain extract (100 ug/mL) and SK-BR-3 cell extract (100 ug/mL). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in antibody diluent CP (ab282211). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-PADI2 / PAD2 antibody [EPR26940-342] - BSA and Azide free (Detector) (AB321828)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-PADI2 / PAD2 antibody [EPR26940-342] - BSA and Azide free (Detector) (AB321828)

Sandwich ELISA of ab321826 with the capture antibody ab321827 dilution at 2 ug/mL and detector antibody ab321828 dilution at 0.5 ug/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26940-342

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody has 48% cross reaction with human PADI3 / PAD3, but does not cross-react with human PADI4 / PAD4.

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Reactivity data

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Product details

ab321828 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human/Mouse PADI2 / PAD2. The recommended pair for sandwich ELISA is:

Capture: ab321827, Human/Mouse PADI2 / PAD2 Capture Antibody (unconjugated)

Detector: ab321828, Human/Mouse PADI2 / PAD2 Detector Antibody (unconjugated)

The reference range value is 0.08-10 ng/mL.

The recommended antibody orientation is based on internal optimization for ELISAbased assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PADI2 also known as PAD2 or PADdeux is a protein that performs post-translational modification by converting arginine residues into citrulline residues in proteins through a process known as citrullination. This protein has an approximate mass of 75 kDa. PADI2 is expressed in many tissues but shows higher levels in the brain skeletal muscle and mammary gland. Within these tissues PADI2 plays a role in regulating various physiological processes by altering protein function through citrullination.
Biological function summary

PADI2 contributes significantly to the regulation of gene expression protein function and signal transduction. PADI2 does not exist as part of a larger protein complex yet it interacts with other proteins to regulate cellular processes. In particular it modifies histones which impacts chromatin structure and gene expression playing a role in cell differentiation and immune response modulation. Its activity can affect various cellular outcomes by regulating the function and interaction of different protein substrates.

Pathways

PADI2 takes part in gene regulation and cell differentiation processes by modifying histones and impacting chromatin structure. It is integral in pathways related to inflammation and immune responses such as the NF-kB signaling pathway enhancing its biological role. PADI2 interacts with multiple proteins including histones and other signaling molecules reinforcing its critical role in these pathways.

PADI2 has been linked to rheumatoid arthritis and multiple sclerosis due to its role in immune modulation and inflammation. In rheumatoid arthritis aberrant citrullination by PADI2 contributes to the production of autoantibodies which are involved in the disease's pathogenesis. It also associates with abnormal neural processes in multiple sclerosis where citrullinated proteins potentially contribute to the demyelination process. In these conditions PADI2's function relates closely with other citrullinating proteins such as PADI4 highlighting the interconnectedness of these pathways in disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the deimination of arginine residues of proteins.
See full target information PADI2
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Product promise

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