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AB172959

Anti-PADI3 / PAD3 antibody [EPR12165(B)]

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(3 Publications)

Rabbit Recombinant Monoclonal PADI3 / PAD3 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

PAD3, PDI3, PADI3, Protein-arginine deiminase type-3, Peptidylarginine deiminase III, Protein-arginine deiminase type III

2 Images
Immunoprecipitation - Anti-PADI3 / PAD3 antibody [EPR12165(B)] (AB172959)
  • IP

Supplier Data

Immunoprecipitation - Anti-PADI3 / PAD3 antibody [EPR12165(B)] (AB172959)

Western blot analysis on immunoprecipitation pellet from Human fetal muscle lysate immunoprecipitated using ab172959 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-PADI3 / PAD3 antibody [EPR12165(B)] (ab172959)

Predicted band size: 75 kDa

false

Western blot - Anti-PADI3 / PAD3 antibody [EPR12165(B)] (AB172959)
  • WB

Supplier Data

Western blot - Anti-PADI3 / PAD3 antibody [EPR12165(B)] (AB172959)

All lanes:

Western blot - Anti-PADI3 / PAD3 antibody [EPR12165(B)] (ab172959) at 1/1000 dilution

Lane 1:

Human fetal muscle lysate at 10 µg

Lane 2:

Human skeletal muscle lysate at 10 µg

Lane 3:

Human skin lysate at 10 µg

Predicted band size: 75 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12165(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PADI3 also known as PAD3 is an enzyme that belongs to the peptidylarginine deiminase family. It converts arginine residues into citrullines in the presence of calcium ions an activity known as citrullination or deimination. PADI3 has a molecular mass of approximately 74 kDa. Expression of this enzyme is found mainly in hair follicles skin and possibly other epithelial tissues where it modifies structural proteins important for tissue organization and function.
Biological function summary

PADI3 plays a role in the formation of the hair shaft by modifying trichohyalin a core structural protein and possibly other substrates within hair matrix keratinocytes. It contributes to the stabilization and structural integrity of hair and skin proteins. Although not massive in function alone PADI3 may work as part of a complex with other proteins to achieve these modifications effectively.

Pathways

PADI3 fits into the intricate web of keratinization and epidermal differentiation processes. It is part of pathways that involve protein deimination alongside proteins such as PAD2 and PAD1 which work together to modify and stabilize intermediate filaments and other structural protein components within the skin and hair.

PADI3 is linked most commonly to hair and skin-related conditions including some forms of alopecia and psoriasis. Studies suggest the enzyme might interact with keratin proteins influencing the abnormal keratinization observed in these disorders. Mutations in PADI3 as well as related proteins like keratin may disrupt normal protein modification and aggregation leading to these pathological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the deimination of arginine residues of proteins.
See full target information PADI3
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cancer biology & medicine 16:729-742 PubMed31908891

2020

PADI3 induces cell cycle arrest the Sirt2/AKT/p21 pathway and acts as a tumor suppressor gene in colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaotian Chang,Zhengbin Chai,Jiaorui Zou,Hongxing Wang,Yao Wang,Yabing Zheng,Hui Wu,Chunyan Liu

Cancer cell international 19:277 PubMed31708688

2019

PADI3 plays an antitumor role via the Hsp90/CKS1 pathway in colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengbin Chai,Li Wang,Yabing Zheng,Na Liang,Xiwei Wang,Yingying Zheng,Zhiwei Zhang,Chuanxi Zhao,Tingting Zhu,Chunyan Liu

American journal of human genetics 99:1292-1304 PubMed27866708

2016

Mutations in Three Genes Encoding Proteins Involved in Hair Shaft Formation Cause Uncombable Hair Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

F Buket Ü Basmanav,Laura Cau,Aylar Tafazzoli,Marie-Claire Méchin,Sabrina Wolf,Maria Teresa Romano,Frederic Valentin,Henning Wiegmann,Anne Huchenq,Rima Kandil,Natalie Garcia Bartels,Arzu Kilic,Susannah George,Damian J Ralser,Stefan Bergner,David J P Ferguson,Ana-Maria Oprisoreanu,Maria Wehner,Holger Thiele,Janine Altmüller,Peter Nürnberg,Daniel Swan,Darren Houniet,Aline Büchner,Lisa Weibel,Nicola Wagner,Ramon Grimalt,Anette Bygum,Guy Serre,Ulrike Blume-Peytavi,Eli Sprecher,Susanne Schoch,Vinzenz Oji,Henning Hamm,Paul Farrant,Michel Simon,Regina C Betz
View all publications
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Product promise

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