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AB187138

Anti-PADI3 / PAD3 antibody [EPR12165(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal PADI3 / PAD3 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

PAD3, PDI3, PADI3, Protein-arginine deiminase type-3, Peptidylarginine deiminase III, Protein-arginine deiminase type III

2 Images
Immunoprecipitation - Anti-PADI3 / PAD3 antibody [EPR12165(B)] - BSA and Azide free (AB187138)
  • IP

Supplier Data

Immunoprecipitation - Anti-PADI3 / PAD3 antibody [EPR12165(B)] - BSA and Azide free (AB187138)

This data was developed using ab172959, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from Human fetal muscle lysate immunoprecipitated using ab172959 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-PADI3 / PAD3 antibody [EPR12165(B)] (<a href='/en-us/products/primary-antibodies/padi3-pad3-antibody-epr12165b-ab172959'>ab172959</a>)

Predicted band size: 75 kDa

false

Western blot - Anti-PADI3 / PAD3 antibody [EPR12165(B)] - BSA and Azide free (AB187138)
  • WB

Supplier Data

Western blot - Anti-PADI3 / PAD3 antibody [EPR12165(B)] - BSA and Azide free (AB187138)

This data was developed using ab172959, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PADI3 / PAD3 antibody [EPR12165(B)] (<a href='/en-us/products/primary-antibodies/padi3-pad3-antibody-epr12165b-ab172959'>ab172959</a>) at 1/1000 dilution

Lane 1:

Human fetal muscle lysate at 10 µg

Lane 2:

Human skeletal muscle lysate at 10 µg

Lane 3:

Human skin lysate at 10 µg

Predicted band size: 75 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12165(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab187138 is the carrier-free version of ab172959.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PADI3 also known as PAD3 is an enzyme that belongs to the peptidylarginine deiminase family. It converts arginine residues into citrullines in the presence of calcium ions an activity known as citrullination or deimination. PADI3 has a molecular mass of approximately 74 kDa. Expression of this enzyme is found mainly in hair follicles skin and possibly other epithelial tissues where it modifies structural proteins important for tissue organization and function.
Biological function summary

PADI3 plays a role in the formation of the hair shaft by modifying trichohyalin a core structural protein and possibly other substrates within hair matrix keratinocytes. It contributes to the stabilization and structural integrity of hair and skin proteins. Although not massive in function alone PADI3 may work as part of a complex with other proteins to achieve these modifications effectively.

Pathways

PADI3 fits into the intricate web of keratinization and epidermal differentiation processes. It is part of pathways that involve protein deimination alongside proteins such as PAD2 and PAD1 which work together to modify and stabilize intermediate filaments and other structural protein components within the skin and hair.

PADI3 is linked most commonly to hair and skin-related conditions including some forms of alopecia and psoriasis. Studies suggest the enzyme might interact with keratin proteins influencing the abnormal keratinization observed in these disorders. Mutations in PADI3 as well as related proteins like keratin may disrupt normal protein modification and aggregation leading to these pathological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the deimination of arginine residues of proteins.
See full target information PADI3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 43:2636-2660 PubMed38778156

2024

The immune response to RNA suppresses nucleic acid synthesis by limiting ribose 5-phosphate.

Applications

Unspecified application

Species

Unspecified reactive species

Pushpak Bhattacharjee,Die Wang,Dovile Anderson,Joshua N Buckler,Eveline de Geus,Feng Yan,Galina Polekhina,Ralf Schittenhelm,Darren J Creek,Lawrence D Harris,Anthony J Sadler
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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