AB183209

Anti-PADI3 / PAD3 antibody - N-terminal

Name: Anti-PADI3 / PAD3 antibody - N-terminal (ab183209) | Abcam
Brand: Abcam Limited
SKU: AB183209
Price: 525 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(3 Publications)

Rabbit Polyclonal PADI3 / PAD3 antibody. N-terminal. Suitable for IP, ELISA, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PADI3.

View Alternative Names

PAD3, PDI3, PADI3, Protein-arginine deiminase type-3, Peptidylarginine deiminase III, Protein-arginine deiminase type III

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP, ICC/IF, IHC-P, ELISA

applications

Immunogen

Synthetic Peptide within Human PADI3. The exact immunogen used to generate this antibody is proprietary information.

Q9ULW8

Specificity

Reacts with 75 kDa PADI3 protein. No cross reaction with the other isoforms.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PADI3 also known as PAD3 is an enzyme that belongs to the peptidylarginine deiminase family. It converts arginine residues into citrullines in the presence of calcium ions an activity known as citrullination or deimination. PADI3 has a molecular mass of approximately 74 kDa. Expression of this enzyme is found mainly in hair follicles skin and possibly other epithelial tissues where it modifies structural proteins important for tissue organization and function.

Biological function summary

PADI3 plays a role in the formation of the hair shaft by modifying trichohyalin a core structural protein and possibly other substrates within hair matrix keratinocytes. It contributes to the stabilization and structural integrity of hair and skin proteins. Although not massive in function alone PADI3 may work as part of a complex with other proteins to achieve these modifications effectively.

Pathways

PADI3 fits into the intricate web of keratinization and epidermal differentiation processes. It is part of pathways that involve protein deimination alongside proteins such as PAD2 and PAD1 which work together to modify and stabilize intermediate filaments and other structural protein components within the skin and hair.

PADI3 is linked most commonly to hair and skin-related conditions including some forms of alopecia and psoriasis. Studies suggest the enzyme might interact with keratin proteins influencing the abnormal keratinization observed in these disorders. Mutations in PADI3 as well as related proteins like keratin may disrupt normal protein modification and aggregation leading to these pathological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the deimination of arginine residues of proteins.

See full target information PADI3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 11:85 PubMed32117246

2020

Citrullination Alters the Antiviral and Immunomodulatory Activities of the Human Cathelicidin LL-37 During Rhinovirus Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Víctor Casanova,Filipa Henderson Sousa,Priyanka Shakamuri,Pavel Svoboda,Chloé Buch,Mathilde D'Acremont,Maria A Christophorou,Jan Pohl,Craig Stevens,Peter G Barlow

The Journal of investigative dermatology 140:959-970.e3 PubMed31669413

2019

Palmitoyl Acyltransferase Activity of ZDHHC13 Regulates Skin Barrier Development Partly by Controlling PADi3 and TGM1 Protein Stability.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Ying Chen,Kuo-Ray Lin,Yi-Ju Chen,Yun-Jung Chiang,Kun-Chin Ho,Li-Fen Shen,I-Wen Song,Kai-Ming Liu,Hsin-Fang Yang-Yen,Yu-Ju Chen,Yuan-Tsong Chen,Fu-Tong Liu,Jeffrey J Y Yen

Arthritis research & therapy 18:239 PubMed27765067

2016

Rheumatoid arthritis antigens homocitrulline and citrulline are generated by local myeloperoxidase and peptidyl arginine deiminases 2, 3 and 4 in rheumatoid nodule and synovial tissue.

Applications

IHC-P

Species

Human

Sanna Turunen,Johanna Huhtakangas,Tomi Nousiainen,Maarit Valkealahti,Jukka Melkko,Juha Risteli,Petri Lehenkari

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PADI3 / PAD3 antibody - N-terminal

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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