AB26071

Anti-PADI4 / PAD4 antibody

Name: Anti-PADI4 / PAD4 antibody (ab26071) | Abcam
Brand: Abcam Limited
SKU: AB26071
Price: 535 USD
Availability: InStock

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(1 Publication)

Goat Polyclonal PADI4 / PAD4 antibody. Suitable for ICC and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PADI4 aa 1-50.

View Alternative Names

PAD4, PADI5, PDI5, PADI4, Protein-arginine deiminase type-4, HL-60 PAD, Peptidylarginine deiminase IV, Protein-arginine deiminase type IV

2 Images

Supplier Data

Immunocytochemistry - Anti-PADI4 / PAD4 antibody (AB26071)

Immunocytochemistry analysis of U2OS cells labeling PADI4 / PAD4 antibody with ab26071 at 10 μg/mL (1 hour incubation). Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. Alexa Fluor^® 488 2 μg/mL was used as the secondary antibody. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG at 10 μg/mL followed by Alexa Fluor^® 488 secondary antibody 2 μg/mL.

Supplier Data

Immunocytochemistry - Anti-PADI4 / PAD4 antibody (AB26071)

Immunocytochemistry analysis of HeLa cells labeling PADI4 / PAD4 antibody with ab26071 at 10 μg/mL (1 hour incubation). Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. Alexa Fluor^® 488 2 μg/mL was used as the secondary antibody. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG at 10 μg/mL followed by Alexa Fluor^® 488 secondary antibody 2 μg/mL.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC

applications

Immunogen

Synthetic Peptide within Human PADI4 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9UM07

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "10 µg/mL", "ICC-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PADI4 also known as PAD4 PADI4 protein or PAD-4 is an enzyme that plays a mechanical role in post-translational modification. It functions by catalyzing the conversion of arginine residues into citrulline in histones a process known as citrullination or deimination. This enzyme exhibits a molecular mass of approximately 74 kDa and is expressed notably in tissues like bone marrow and sites of inflammation. PADI4 shows high expression in immune cells such as neutrophils which are important for inflammatory responses.

Biological function summary

The PADI4 protein impacts gene expression by modifying chromatin architecture making it part of chromatin remodeling activities. PADI4 is known to associate with chromatin but it does not form large stable complexes. It influences the transcriptional activity of genes by altering histone interactions which can affect immune responses and cellular differentiation. Through these chromatin changes PADI4 plays a notable role in regulating genes associated with immune function and inflammatory responses.

Pathways

PADI4 integrates into the inflammatory response and apoptosis pathways. It influences apoptosis by modulating pro-inflammatory gene expression and participating in the formation of neutrophil extracellular traps (NETs). PADI4 works with other proteins such as histone H3 and histone H4 to influence immune cell pathways. These interactions underline its role in the regulation of protein expressions during inflammation and immune-mediated processes.

PADI4 connects to rheumatoid arthritis (RA) and certain cancers. In rheumatoid arthritis altered activity of PADI4 can lead to the production of autoantibodies against citrullinated proteins which exacerbates inflammation in joints. The enzyme’s dysregulation is also implicated in cancer progression where it may affect tumor growth and metastasis. PADI4’s interaction with other proteins like citrullinated histones highlights its association with pathogenic processes in such diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance (PubMed : 15339660, PubMed : 15345777, PubMed : 16567635, PubMed : 21245532). Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci) (PubMed : 15339660, PubMed : 15345777, PubMed : 16567635, PubMed : 21245532). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1 : citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance (PubMed : 15339660, PubMed : 15345777, PubMed : 16567635, PubMed : 21245532). Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci (PubMed : 18209087). Required for the formation of neutrophil extracellular traps (NETs); NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription (PubMed : 15345777). Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1 (PubMed : 15731352).

See full target information PADI4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Arthritis and rheumatism 58:2287-95 PubMed18668562

2008

Synovial fluid is a site of citrullination of autoantigens in inflammatory arthritis.

Applications

Species

Human

Andrew Kinloch,Karin Lundberg,Robin Wait,Natalia Wegner,Ngee Han Lim,Albert J W Zendman,Tore Saxne,Vivianne Malmström,Patrick J Venables

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PADI4 / PAD4 antibody

Immunocytochemistry - Anti-PADI4 / PAD4 antibody (AB26071)

Immunocytochemistry - Anti-PADI4 / PAD4 antibody (AB26071)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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