Anti-PADI4 / PAD4 antibody (ab96758) is a rabbit polyclonal antibody that is used to detect PADI4 / PAD4 in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse samples.
- Over 10 publications
- Trusted since 2010
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- Journal of neuroinflammation 20:2222023Inhibition of neutrophil extracellular trap formation ameliorates neuroinflammation and neuronal apoptosis via STING-dependent IRE1α/ASK1/JNK signaling pathway in mice with traumatic brain injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuihong Shi,Liang Liu,Yiyao Cao,Guangshuo Ma,Yanlin Zhu,Jianye Xu,Xu Zhang,Tuo Li,Liang Mi,Haoran Jia,Yanfeng Zhang,Xilei Liu,Yuan Zhou,Shenghui Li,Guili Yang,Xiao Liu,Fanglian Chen,Baolong Wang,Quanjun Deng,Shu Zhang,Jianning ZhangPubMed 37777772
- Cell death & disease 14:3742023Neutrophil extracellular traps aggravate neuronal endoplasmic reticulum stress and apoptosis via TLR9 after traumatic brain injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang Mi,Xiaobin Min,Mingming Shi,Liang Liu,Yanfeng Zhang,Yanlin Zhu,Peng Li,Yan Chai,Fanglian Chen,Quanjun Deng,Shu Zhang,Jianning Zhang,Xin ChenPubMed 37365190
- The journal of gene medicine 25:e35302023GSK484, an inhibitor of peptidyl arginine deiminase 4, increases the radiosensitivity of colorectal cancer and inhibits neutrophil extracellular traps.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBinbin Wang,Xiaojuan Su,Bo Zhang,Shiwen PanPubMed 37203323
- Experimental & molecular medicine 55:939-9512023Mesenchymal stem cell-derived extracellular vesicles protect against abdominal aortic aneurysm formation by inhibiting NET-induced ferroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang Chen,Yuting Liu,Zheyu Wang,Leiyang Zhang,Yi Xu,Yinan Li,Lan Zhang,Guiming Wang,Shuofei Yang,Guanhua XuePubMed 37121969
- Frontiers in immunology 14:11257592023Inhibition of neutrophil extracellular trap formation attenuates NLRP1-dependent neuronal pyroptosis STING/IRE1α pathway after traumatic brain injury in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYiyao Cao,Mingming Shi,Liang Liu,Yan Zuo,Haoran Jia,Xiaobin Min,Xilei Liu,Zhijuan Chen,Yuan Zhou,Shenghui Li,Guili Yang,Xiao Liu,Quanjun Deng,Fanglian Chen,Xin Chen,Shu Zhang,Jianning ZhangPubMed 37143681
- BMC medicine 20:3642022Neutrophil degranulation and severely impaired extracellular trap formation at the basis of susceptibility to infections of hemodialysis patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSalti Talal,Khoury Mona,Awad Karem,Lerenthal Yaniv,Harari-Misgav Reut,Shemesh Ariel,Avraham-Kelbert Moran,Eitam Harel,Salvatore Campisi-Pinto,Abu-Amna Mahmoud,Colodner Raul,Tovbin David,Bar-Sela Gil,Cohen IdanPubMed 36284314
- International journal of molecular sciences 23:2022Metformin Mitigated Obesity-Driven Cancer Aggressiveness in Tumor-Bearing Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChun-Jung Chen,Chih-Cheng Wu,Cheng-Yi Chang,Jian-Ri Li,Yen-Chuan Ou,Wen-Ying Chen,Su-Lan Liao,Jiaan-Der WangPubMed 36012397
- Theranostics 11:9431-94512021Interruption of neutrophil extracellular traps formation dictates host defense and tubular HOXA5 stability to augment efficacy of anti-Fn14 therapy against septic AKI.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYin Ni,Bang-Chuan Hu,Guo-Hua Wu,Zi-Qiang Shao,Yang Zheng,Run Zhang,Jun Jin,Jun Hong,Xiang-Hong Yang,Ren-Hua Sun,Jin-Quan Liu,Shi-Jing MoPubMed 34646379
- Nature aging 1:715-7332021Multi-omic profiling of primary mouse neutrophils predicts a pattern of sex and age-related functional regulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRyan J Lu,Shalina Taylor,Kévin Contrepois,Minhoo Kim,Juan I Bravo,Mathew Ellenberger,Nirmal K Sampathkumar,Bérénice A BenayounPubMed 34514433
- Nature communications 12:34812021TcpC inhibits neutrophil extracellular trap formation by enhancing ubiquitination mediated degradation of peptidylarginine deiminase 4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Ou,Jia-Qi Fang,Zhe-Sheng Zhang,Zhe Chi,Jie Fang,Di-Yan Xu,Kai-Zhong Lu,Meng-Qing Qian,Da-Yong Zhang,Jun-Ping Guo,Wei Gao,Na-Ru Zhang,Jian-Ping PanPubMed 34108482
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PADI4 aa 300-600. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UM07
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/10000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance (PubMed:15339660, PubMed:15345777, PubMed:16567635, PubMed:21245532). Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci) (PubMed:15339660, PubMed:15345777, PubMed:16567635, PubMed:21245532). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance (PubMed:15339660, PubMed:15345777, PubMed:16567635, PubMed:21245532). Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci (PubMed:18209087). Required for the formation of neutrophil extracellular traps (NETs); NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription (PubMed:15345777). Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1 (PubMed:15731352).
Alternative names
PAD4, PADI5, PDI5, PADI4, Protein-arginine deiminase type-4, HL-60 PAD, Peptidylarginine deiminase IV, Protein-arginine deiminase type IV
Recommended products
Anti-PADI4 / PAD4 antibody (ab96758) is a rabbit polyclonal antibody that is used to detect PADI4 / PAD4 in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse samples.
- Over 10 publications
- Trusted since 2010
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PADI4 aa 300-600. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UM07
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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5 product images
Western blot - Anti-PADI4 / PAD4 antibody (ab96758)
7.5% SDS-PAGE
All lanes: Western blot - Anti-PADI4 / PAD4 antibody (ab96758) at 1/5000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Lane 2: PAD4-transfected HEK-293T whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 74 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody (ab96758)
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft tissue staining PADI4/PAD4 with ab96758 at 1/100 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
Immunocytochemistry/ Immunofluorescence - Anti-PADI4 / PAD4 antibody (ab96758)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) whole cells
labeling PADI4/PAD4 with ab96758 at 1/200 dilution.
Western blot - Anti-PADI4 / PAD4 antibody (ab96758)
7.5% SDS-PAGE
All lanes: Western blot - Anti-PADI4 / PAD4 antibody (ab96758) at 1/1000 dilution
All lanes: Human spleen tissue extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 74 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody (ab96758)
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue staining PADI4/PAD4 at cytoplasm with ab96758 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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