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Rabbit Polyclonal PADI4 / PAD4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PADI4 aa 300-600.


Images

Western blot - Anti-PADI4 / PAD4 antibody (AB96758), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody (AB96758), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PADI4 / PAD4 antibody (AB96758), expandable thumbnail
  • Western blot - Anti-PADI4 / PAD4 antibody (AB96758), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody (AB96758), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human PADI4 aa 300-600. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UM07

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/10000.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance (PubMed:15339660, PubMed:15345777, PubMed:16567635, PubMed:21245532). Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci) (PubMed:15339660, PubMed:15345777, PubMed:16567635, PubMed:21245532). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance (PubMed:15339660, PubMed:15345777, PubMed:16567635, PubMed:21245532). Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci (PubMed:18209087). Required for the formation of neutrophil extracellular traps (NETs); NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription (PubMed:15345777). Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1 (PubMed:15731352).

Alternative names

Recommended products

Rabbit Polyclonal PADI4 / PAD4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PADI4 aa 300-600.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human PADI4 aa 300-600. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UM07
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PADI4 also known as PAD4 PADI4 protein or PAD-4 is an enzyme that plays a mechanical role in post-translational modification. It functions by catalyzing the conversion of arginine residues into citrulline in histones a process known as citrullination or deimination. This enzyme exhibits a molecular mass of approximately 74 kDa and is expressed notably in tissues like bone marrow and sites of inflammation. PADI4 shows high expression in immune cells such as neutrophils which are important for inflammatory responses.

Biological function summary

The PADI4 protein impacts gene expression by modifying chromatin architecture making it part of chromatin remodeling activities. PADI4 is known to associate with chromatin but it does not form large stable complexes. It influences the transcriptional activity of genes by altering histone interactions which can affect immune responses and cellular differentiation. Through these chromatin changes PADI4 plays a notable role in regulating genes associated with immune function and inflammatory responses.

Pathways

PADI4 integrates into the inflammatory response and apoptosis pathways. It influences apoptosis by modulating pro-inflammatory gene expression and participating in the formation of neutrophil extracellular traps (NETs). PADI4 works with other proteins such as histone H3 and histone H4 to influence immune cell pathways. These interactions underline its role in the regulation of protein expressions during inflammation and immune-mediated processes.

Associated diseases and disorders

PADI4 connects to rheumatoid arthritis (RA) and certain cancers. In rheumatoid arthritis altered activity of PADI4 can lead to the production of autoantibodies against citrullinated proteins which exacerbates inflammation in joints. The enzyme’s dysregulation is also implicated in cancer progression where it may affect tumor growth and metastasis. PADI4’s interaction with other proteins like citrullinated histones highlights its association with pathogenic processes in such diseases.

Product promise

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5 product images

  • Western blot - Anti-PADI4 / PAD4 antibody (ab96758), expandable thumbnail

    Western blot - Anti-PADI4 / PAD4 antibody (ab96758)

    7.5% SDS-PAGE

    All lanes: Western blot - Anti-PADI4 / PAD4 antibody (ab96758) at 1/5000 dilution

    Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

    Lane 2: PAD4-transfected HEK-293T whole cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 74 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody (ab96758), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody (ab96758)

    Immunohistochemical analysis of paraffin-embedded Cal27 xenograft tissue staining PADI4/PAD4 with ab96758 at 1/100 dilution.

    Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.

  • Immunocytochemistry/ Immunofluorescence - Anti-PADI4 / PAD4 antibody (ab96758), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PADI4 / PAD4 antibody (ab96758)

    Immunofluorescence analysis of paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) whole cells
    labeling PADI4/PAD4 with ab96758 at 1/200 dilution.

  • Western blot - Anti-PADI4 / PAD4 antibody (ab96758), expandable thumbnail

    Western blot - Anti-PADI4 / PAD4 antibody (ab96758)

    7.5% SDS-PAGE

    All lanes: Western blot - Anti-PADI4 / PAD4 antibody (ab96758) at 1/1000 dilution

    All lanes: Human spleen tissue extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 74 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody (ab96758), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody (ab96758)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue staining PADI4/PAD4 at cytoplasm with ab96758 at 1/500 dilution.

    Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.

Downloads

Product protocols

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