AB96758

Anti-PADI4 / PAD4 antibody

Name: Anti-PADI4 / PAD4 antibody (ab96758) | Abcam
Brand: Abcam Limited
SKU: AB96758
Price: 350 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(21 Publications)

Anti-PADI4 / PAD4 antibody (ab96758) is a rabbit polyclonal antibody detecting PADI4 / PAD4 in Western Blot, IHC-P. Suitable for Human.

- Over 10 publications
- Trusted since 2010

View Alternative Names

PAD4, PADI5, PDI5, PADI4, Protein-arginine deiminase type-4, HL-60 PAD, Peptidylarginine deiminase IV, Protein-arginine deiminase type IV

4 Images

Supplier Data

Western blot - Anti-PADI4 / PAD4 antibody (AB96758)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-PADI4 / PAD4 antibody (ab96758) at 1/5000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

PAD4-transfected HEK-293T whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 74 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody (AB96758)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody (AB96758)

Immunohistochemical analysis of paraffin-embedded human lymph node tissue staining PAD4 with ab96758 at 1/500 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody (AB96758)

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft tissue staining PADI4/PAD4 with ab96758 at 1/100 dilution.

Antigen Retrieval : EDTA based buffer, pH 8.0, 15min.

Supplier Data

Western blot - Anti-PADI4 / PAD4 antibody (AB96758)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-PADI4 / PAD4 antibody (ab96758) at 1/1000 dilution

All lanes:

Human spleen tissue extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 74 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PADI4 aa 300-600. The exact immunogen used to generate this antibody is proprietary information.

Q9UM07

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-PADI4 / PAD4 antibody (ab96758) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of PADI4 / PAD4?
Anti-PADI4 / PAD4 (ab96758) specifically detects a band for PADI4 / PAD4 (UniProt: Q9UM07) at a molecular weight of 74kDa.

Trusted by the scientific community
Anti-PADI4 / PAD4 (ab96758) was first used in a scientific publication in 2010 and has been cited over 10 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PADI4 also known as PAD4 PADI4 protein or PAD-4 is an enzyme that plays a mechanical role in post-translational modification. It functions by catalyzing the conversion of arginine residues into citrulline in histones a process known as citrullination or deimination. This enzyme exhibits a molecular mass of approximately 74 kDa and is expressed notably in tissues like bone marrow and sites of inflammation. PADI4 shows high expression in immune cells such as neutrophils which are important for inflammatory responses.

Biological function summary

The PADI4 protein impacts gene expression by modifying chromatin architecture making it part of chromatin remodeling activities. PADI4 is known to associate with chromatin but it does not form large stable complexes. It influences the transcriptional activity of genes by altering histone interactions which can affect immune responses and cellular differentiation. Through these chromatin changes PADI4 plays a notable role in regulating genes associated with immune function and inflammatory responses.

Pathways

PADI4 integrates into the inflammatory response and apoptosis pathways. It influences apoptosis by modulating pro-inflammatory gene expression and participating in the formation of neutrophil extracellular traps (NETs). PADI4 works with other proteins such as histone H3 and histone H4 to influence immune cell pathways. These interactions underline its role in the regulation of protein expressions during inflammation and immune-mediated processes.

PADI4 connects to rheumatoid arthritis (RA) and certain cancers. In rheumatoid arthritis altered activity of PADI4 can lead to the production of autoantibodies against citrullinated proteins which exacerbates inflammation in joints. The enzyme’s dysregulation is also implicated in cancer progression where it may affect tumor growth and metastasis. PADI4’s interaction with other proteins like citrullinated histones highlights its association with pathogenic processes in such diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance (PubMed : 15339660, PubMed : 15345777, PubMed : 16567635, PubMed : 21245532). Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci) (PubMed : 15339660, PubMed : 15345777, PubMed : 16567635, PubMed : 21245532). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1 : citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance (PubMed : 15339660, PubMed : 15345777, PubMed : 16567635, PubMed : 21245532). Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci (PubMed : 18209087). Required for the formation of neutrophil extracellular traps (NETs); NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription (PubMed : 15345777). Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1 (PubMed : 15731352).

See full target information PADI4

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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 15:9221-9239 PubMed40963908

2025

Neutrophil extracellular traps induce endothelial damage and exacerbate vasospasm in traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jinchao Wang,Lei Li,Jianye Xu,Dilmurat Gheyret,Kaiji Li,Xu Zhang,Haoran Jia,Ye Tian,Xiao Liu,Shenghui Li,Guili Yang,Yalong Gao,Ruilong Peng,Huajie Liu,Bin Liu,Jianfeng Zhuang,Cong Wang,Xin Chen,Yafan Liu,Bo Chen,Chuan Huang,Yuhan Li,Xin Chen,Jianning Zhang,Shu Zhang

Cell communication and signaling : CCS 23:317 PubMed40598174

2025

IL-23 promotes neuronal ferroptosis via IL-23R/STAT3 signaling after traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Chen,Guihong Shi,Jianye Xu,Xu Zhang,Yanlin Zhu,Lei Li,Cong Wang,Dilmurat Gheyret,Jinchao Wang,Xilei Liu,Yiyao Cao,Rui Tan,Yuan Zhou,RongCai Jiang,Shenghui Li,Tuo Li,Xiao Liu,Xin Chen,Guili Yang,Jianning Zhang,Shu Zhang

iScience 28:112763 PubMed40585508

2025

Gut microbiota from voluntary exercised mice protects the intestinal barrier by inhibiting neutrophil extracellular trap formation.

Applications

Unspecified application

Species

Unspecified reactive species

Beibei Zhu,Hao Wu,Han Zhang,Qi Song,Yong Xiao,Baoping Yu

Drug delivery 32:2486840 PubMed40230297

2025

Constructed transferrin receptor-targeted liposome for the delivery of fluvoxamine to improve prognosis in a traumatic brain injury mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Mi,Jiangyuan Yuan,Yuxing Jiang,Yuqian Hu,Chuanxiang Lv,Yongqiang Xu,Mingqi Liu,Tao Liu,Xuanhui Liu,Jinhao Huang,Rongcai Jiang,Wei Quan

Inflammation 48:2756-2771 PubMed39725843

2024

Rhaponticin Alleviates Collagen-induced Arthritis by Inhibiting NLRP3/GSDMD-mediated Neutrophil Extracellular Traps.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Zhang,Xinyue Xie,Qinhao Shen,Chenchen Yuan,Guotao Lu,Weiming Xiao,Weijuan Gong,Xiaoyan Fu,Xuebing Feng

Redox biology 74:103231 PubMed38861835

2024

Cathepsin C from extracellular histone-induced M1 alveolar macrophages promotes NETosis during lung ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Yu,Yu Fu,Jiameng Gao,Qingqing Zhang,Nan Zhang,Zhiyuan Zhang,Xuemei Jiang,Chang Chen,Zongmei Wen

Journal of immunology (Baltimore, Md. : 1950) 213:75-85 PubMed38758115

2024

Neutrophil-Derived Peptidyl Arginine Deiminase Activity Contributes to Pulmonary Emphysema by Enhancing Elastin Degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Mark P Murphy,David Hunt,Malcolm Herron,Jake McDonnell,Rashed Alshuhoumi,Lorcan P McGarvey,Aurelie Fabré,Helen O'Brien,Cormac McCarthy,S Lorraine Martin,Noel G McElvaney,Emer P Reeves

Cell communication and signaling : CCS 22:96 PubMed38308301

2024

NLRP3 exacerbates EAE severity through ROS-dependent NET formation in the mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Da Jeong Byun,Jaeho Lee,Kyungryung Ko,Young-Min Hyun

Journal of neuroinflammation 20:222 PubMed37777772

2023

Inhibition of neutrophil extracellular trap formation ameliorates neuroinflammation and neuronal apoptosis via STING-dependent IRE1α/ASK1/JNK signaling pathway in mice with traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Guihong Shi,Liang Liu,Yiyao Cao,Guangshuo Ma,Yanlin Zhu,Jianye Xu,Xu Zhang,Tuo Li,Liang Mi,Haoran Jia,Yanfeng Zhang,Xilei Liu,Yuan Zhou,Shenghui Li,Guili Yang,Xiao Liu,Fanglian Chen,Baolong Wang,Quanjun Deng,Shu Zhang,Jianning Zhang

Cell death & disease 14:374 PubMed37365190

2023

Neutrophil extracellular traps aggravate neuronal endoplasmic reticulum stress and apoptosis via TLR9 after traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Mi,Xiaobin Min,Mingming Shi,Liang Liu,Yanfeng Zhang,Yanlin Zhu,Peng Li,Yan Chai,Fanglian Chen,Quanjun Deng,Shu Zhang,Jianning Zhang,Xin Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PADI4 / PAD4 antibody

Western blot - Anti-PADI4 / PAD4 antibody (AB96758)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody (AB96758)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody (AB96758)

Western blot - Anti-PADI4 / PAD4 antibody (AB96758)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (21)

Product promise