Anti-PADI4 / PAD4 antibody [EPR20706]
- BOND RX™ Validated
- RabMAb
- Recombinant
- KO Validated
- What is this?
5
(1 Review)
|
(44 Publications)
Anti-PADI4 / PAD4 antibody [EPR20706] (ab214810) is a rabbit monoclonal antibody detecting PADI4 / PAD4 in Western Blot, IHC-P. Suitable for Mouse, Rat.
- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications
View Alternative Names
Pad4, Pdi4, Padi4, Protein-arginine deiminase type-4, Peptidylarginine deiminase IV, Protein-arginine deiminase type IV
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] (AB214810)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling PADI4 / PAD4 with ab214810 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Strongly nuclear staining on peripheral blood granulocytes of mouse spleen (PMID : 20733033, PMID : 28281906, PMID : 12393868, PMID : 12833157). Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] (AB214810)
Immunohistochemical analysis of paraffin-embedded (A) Spleen tissue from wild-type C57BL/6J mice and (B) Spleen tissue from Padi4 knockout mice labelling PADI4 / PAD4 with ab214810 at 1/500 (2.052 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on (A) Spleen tissue from wild-type C57BL/6J mice, no staining on (B) Spleen tissue from Padi4 knockout mice. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Padi4-KO homozygous mice (Strain ID : T011513).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] (AB214810)
Immunohistochemical analysis of paraffin-embedded (A) Lung tissue from wild-type C57BL/6J mice (B) Lung tissue from Padi4 knockout mice labelling PADI4 / PAD4 with ab214810 at 1/500 (2.052 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on (A) lung tissue from wild-type C57BL/6J mice, no staining on (B) lung tissue from Padi4 knockout mice. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Padi4-KO homozygous mice (Strain ID : T011513).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] (AB214810)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling PADI4 / PAD4 with ab214810 at 1/100 (11.55 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Nuclear staining on granulocytes in mouse spleen.The section was incubated with ab214810 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] (AB214810)
Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling PADI4 / PAD4 with ab214810 at 1/100 (11.55 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Nuclear staining on granulocytes in rat lung.The section was incubated with ab214810 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] (AB214810)
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling PADI4 / PAD4 with ab214810 at 1/100 (11.55 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Nuclear staining on granulocytes in mouse lung.The section was incubated with ab214810 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
- WB
Lab
Western blot - Anti-PADI4 / PAD4 antibody [EPR20706] (AB214810)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Padi4-KO homozygous mice (Strain ID : T011513).
All lanes:
Western blot - Anti-PADI4 / PAD4 antibody [EPR20706] (ab214810) at 1/1000 dilution
Lane 1:
Wild-type mouse spleen tissue lysate (female) at 40 µg
Lane 2:
Wild-type mouse spleen tissue lysate (male) at 40 µg
Lane 3:
Padi4 knockout mouse spleen tissue lysate (male case1) at 40 µg
Lane 4:
Padi4 knockout mouse spleen tissue lysate (male case2) at 40 µg
Lane 5:
Wild-type mouse lung tissue lysate (female) at 40 µg
Lane 6:
Wild-type mouse lung tissue lysate (male) at 40 µg
Lane 7:
Padi4 knockout mouse lung tissue lysate (male case1) at 40 µg
Lane 8:
Padi4 knockout mouse lung tissue lysate (male case2) 40μg at 40 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 74 kDa
false
Exposure time: 180s
- WB
Supplier Data
Western blot - Anti-PADI4 / PAD4 antibody [EPR20706] (AB214810)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-PADI4 / PAD4 antibody [EPR20706] (ab214810) at 1/1000 dilution
Lane 1:
Mouse embryo lysate at 20 µg
Lane 2:
ES-D3 (mouse embryonic multipotent stem cell), whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 74 kDa
true
Exposure time: 3min
- WB
AbReview62868****
Western blot - Anti-PADI4 / PAD4 antibody [EPR20706] (AB214810)
All lanes:
Western blot - Anti-PADI4 / PAD4 antibody [EPR20706] (ab214810) at 1/1000 dilution
Lane 1:
Wild-type mouse embryonic stem cells (E14) at 40 µg with BSA
Lanes 2 - 3:
mouse embryonic stem cells (E14) PADI4 Knock out at 40 µg with BSA
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab6721'>ab6721</a>) at 1/5000 dilution
Predicted band size: 74 kDa
true
Exposure time: 18hr
This image is courtesy of an abreview by Maria Christophorou
- WB
Supplier Data
Western blot - Anti-PADI4 / PAD4 antibody [EPR20706] (AB214810)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-PADI4 / PAD4 antibody [EPR20706] (ab214810) at 1/1000 dilution
Lane 1:
HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (vector control) containing a myc-His-tag®, whole cell lysate at 10 µg
Lane 2:
HEK-293T transfected with mouse PADI4 / PAD4 expression vector containing a myc-His-tag®, whole cell lysate at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 74 kDa
Observed band size: 74 kDa
true
Exposure time: 1s
Related conjugates and formulations (1)
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Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free
Reactivity data
Product details
What is this antibody validated in?
Anti-PADI4 / PAD4 antibody [EPR20706] (ab214810) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Mouse, Rat samples.
What is the molecular weight of PADI4 / PAD4?
Anti-PADI4 / PAD4 [EPR20706] (ab214810) specifically detects a band for PADI4 / PAD4 (UniProt: Q9Z183) at a molecular weight of 74kDa.
Trusted by the scientific community
Anti-PADI4 / PAD4 [EPR20706] (ab214810) was first used in a scientific publication in 2017 and has been cited over 20 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 20µl. Discover our selection of trial-size antibodies.
Specificity confirmed
The specificity of Anti-PADI4 / PAD4 antibody [EPR20706] (ab214810) has been confirmed by Western blot testing in Padi4 Knockout mESCs.
Other related products
We have a range of other formats of antibody clone [EPR20706] also available for your convenience: ab214810, Carrier free - ab227891
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The PADI4 protein impacts gene expression by modifying chromatin architecture making it part of chromatin remodeling activities. PADI4 is known to associate with chromatin but it does not form large stable complexes. It influences the transcriptional activity of genes by altering histone interactions which can affect immune responses and cellular differentiation. Through these chromatin changes PADI4 plays a notable role in regulating genes associated with immune function and inflammatory responses.
Pathways
PADI4 integrates into the inflammatory response and apoptosis pathways. It influences apoptosis by modulating pro-inflammatory gene expression and participating in the formation of neutrophil extracellular traps (NETs). PADI4 works with other proteins such as histone H3 and histone H4 to influence immune cell pathways. These interactions underline its role in the regulation of protein expressions during inflammation and immune-mediated processes.
Product protocols
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Target data
Publications (44)
Recent publications for all applications. Explore the full list and refine your search
Molecular medicine (Cambridge, Mass.) 31:304 PubMed41023796
2025
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International dental journal 75:100958 PubMed40902508
2025
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Nature communications 16:7435 PubMed40796783
2025
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Regenerative therapy 30:241-251 PubMed40654518
2025
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Scientific reports 15:16731 PubMed40369118
2025
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Frontiers in immunology 16:1567522 PubMed40134435
2025
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Iranian journal of pharmaceutical research : IJPR 23:e148139 PubMed40066121
2025
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Cell death & disease 16:60 PubMed39890818
2025
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Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2407346 PubMed39499780
2024
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Pulmonary circulation 14:e12454 PubMed39386377
2024
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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