AB227891

Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal PADI4 / PAD4 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Transfected cell lysate - Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Pad4, Pdi4, Padi4, Protein-arginine deiminase type-4, Peptidylarginine deiminase IV, Protein-arginine deiminase type IV

10 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (AB227891)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (AB227891)

This data was developed using ab214810, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling PADI4 / PAD4 with ab214810 at 1/100 (11.55 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on granulocytes in rat lung.The section was incubated with ab214810 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (AB227891)

This data was developed using ab214810, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) Spleen tissue from wild-type C57BL/6J mice and (B) Spleen tissue from Padi4 knockout mice labelling PADI4 / PAD4 with ab214810 at 1/500 (2.052 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) Spleen tissue from wild-type C57BL/6J mice, no staining on (B) Spleen tissue from Padi4 knockout mice. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Padi4-KO homozygous mice (Strain ID : T011513).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (AB227891)

This data was developed using ab214810, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling PADI4 / PAD4 with ab214810 at 1/100 (11.55 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on granulocytes in mouse lung.The section was incubated with ab214810 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (AB227891)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling PADI4 / PAD4 with ab214810 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Strongly nuclear staining on peripheral blood granulocytes of mouse spleen (PMID : 20733033, PMID : 28281906, PMID : 12393868, PMID : 12833157). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214810).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (AB227891)

This data was developed using ab214810, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling PADI4 / PAD4 with ab214810 at 1/100 (11.55 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on granulocytes in mouse spleen.The section was incubated with ab214810 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (AB227891)

This data was developed using ab214810, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) Lung tissue from wild-type C57BL/6J mice (B) Lung tissue from Padi4 knockout mice labelling PADI4 / PAD4 with ab214810 at 1/500 (2.052 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) lung tissue from wild-type C57BL/6J mice, no staining on (B) lung tissue from Padi4 knockout mice. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Padi4-KO homozygous mice (Strain ID : T011513).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

AbReview62868****

Western blot - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (AB227891)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214810).

All lanes:

Western blot - Anti-PADI4 / PAD4 antibody [EPR20706] (<a href='/en-us/products/primary-antibodies/padi4-pad4-antibody-epr20706-ab214810'>ab214810</a>) at 1/1000 dilution

Lane 1:

Wild-type mouse embryonic stem cells (E14) at 40 µg

Lanes 2 - 3:

mouse embryonic stem cells (E14) PADI4 Knockout at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab6721'>ab6721</a>) at 1/5000 dilution

Predicted band size: 74 kDa

true

Exposure time: 18hr

This image is courtesy of an abreview by Maria Christophorou

Supplier Data

Western blot - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (AB227891)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214810).

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PADI4 / PAD4 antibody [EPR20706] (<a href='/en-us/products/primary-antibodies/padi4-pad4-antibody-epr20706-ab214810'>ab214810</a>) at 1/1000 dilution

Lane 1:

Mouse embryo lysate at 20 µg

Lane 2:

ES-D3 (mouse embryonic multipotent stem cell), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 74 kDa

Observed band size: 74 kDa

true

Exposure time: 3min

Lab

Western blot - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (AB227891)

This data was developed using ab214810, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Padi4-KO homozygous mice (Strain ID : T011513).

All lanes:

Western blot - Anti-PADI4 / PAD4 antibody [EPR20706] (<a href='/en-us/products/primary-antibodies/padi4-pad4-antibody-epr20706-ab214810'>ab214810</a>) at 1/1000 dilution

Lane 1:

Wild-type mouse spleen tissue lysate (female) at 40 µg

Lane 2:

Wild-type mouse spleen tissue lysate (male) at 40 µg

Lane 3:

Padi4 knockout mouse spleen tissue lysate (male case1) at 40 µg

Lane 4:

Padi4 knockout mouse spleen tissue lysate (male case2) at 40 µg

Lane 5:

Wild-type mouse lung tissue lysate (female) at 40 µg

Lane 6:

Wild-type mouse lung tissue lysate (male) at 40 µg

Lane 7:

Padi4 knockout mouse lung tissue lysate (male case1) at 40 µg

Lane 8:

Padi4 knockout mouse lung tissue lysate (male case2) 40μg at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 74 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (AB227891)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214810).

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PADI4 / PAD4 antibody [EPR20706] (<a href='/en-us/products/primary-antibodies/padi4-pad4-antibody-epr20706-ab214810'>ab214810</a>) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (vector control) containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 2:

HEK-293T transfected with mouse PADI4 / PAD4 expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 74 kDa

Observed band size: 74 kDa

true

Exposure time: 1s

Unconjugated

Anti-PADI4 / PAD4 antibody [EPR20706]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20706

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "We have been unable to obtain a sucessful result in IP with ab210747." }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "We have been unable to obtain a sucessful result in IP with ab210747." }, "Transfected cell lysate - Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

ab227891 is the carrier-free version of ab214810.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PADI4 also known as PAD4 PADI4 protein or PAD-4 is an enzyme that plays a mechanical role in post-translational modification. It functions by catalyzing the conversion of arginine residues into citrulline in histones a process known as citrullination or deimination. This enzyme exhibits a molecular mass of approximately 74 kDa and is expressed notably in tissues like bone marrow and sites of inflammation. PADI4 shows high expression in immune cells such as neutrophils which are important for inflammatory responses.

Biological function summary

The PADI4 protein impacts gene expression by modifying chromatin architecture making it part of chromatin remodeling activities. PADI4 is known to associate with chromatin but it does not form large stable complexes. It influences the transcriptional activity of genes by altering histone interactions which can affect immune responses and cellular differentiation. Through these chromatin changes PADI4 plays a notable role in regulating genes associated with immune function and inflammatory responses.

Pathways

PADI4 integrates into the inflammatory response and apoptosis pathways. It influences apoptosis by modulating pro-inflammatory gene expression and participating in the formation of neutrophil extracellular traps (NETs). PADI4 works with other proteins such as histone H3 and histone H4 to influence immune cell pathways. These interactions underline its role in the regulation of protein expressions during inflammation and immune-mediated processes.

PADI4 connects to rheumatoid arthritis (RA) and certain cancers. In rheumatoid arthritis altered activity of PADI4 can lead to the production of autoantibodies against citrullinated proteins which exacerbates inflammation in joints. The enzyme’s dysregulation is also implicated in cancer progression where it may affect tumor growth and metastasis. PADI4’s interaction with other proteins like citrullinated histones highlights its association with pathogenic processes in such diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance (PubMed : 15339660, PubMed : 32528174). Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci) (PubMed : 15339660). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1 : citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance (PubMed : 24463520, PubMed : 32528174). Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci (PubMed : 20733033, PubMed : 23650392). Required for the formation of neutrophil extracellular traps (NETs); NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (PubMed : 20733033, PubMed : 32528174). Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription (By similarity). Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1 (By similarity).

See full target information Padi4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Gerontology 70:1294-1304 PubMed39413738

2024

LncRNA MALAT1 Facilitates Parkinson's Disease Progression by Increasing SOCS3 Promoter Methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuqi Liu,Dan Feng,Fenfen Liu,Yun Liu,Fangya Zuo,Yujie Wang,Lanlan Chen,Xiuhong Guo,Jinyong Tian

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (AB227891)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (AB227891)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (AB227891)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (AB227891)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (AB227891)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (AB227891)

Western blot - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (AB227891)

Western blot - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (AB227891)

Western blot - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (AB227891)

Western blot - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (AB227891)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise