Rabbit Recombinant Monoclonal PADI4 / PAD4 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Transfected cell lysate - Mouse, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (AB227891), expandable thumbnail](https://content.abcam.com/products/images/padi4-pad4-antibody-epr20706-bsa-and-azide-free-ab227891--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img71801.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (AB227891)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (AB227891), expandable thumbnail](https://content.abcam.com/products/images/padi4-pad4-antibody-epr20706-bsa-and-azide-free-ab227891--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102642.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (AB227891)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (AB227891), expandable thumbnail](https://content.abcam.com/products/images/padi4-pad4-antibody-epr20706-bsa-and-azide-free-ab227891--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img66846.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (AB227891)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (AB227891), expandable thumbnail](https://content.abcam.com/products/images/padi4-pad4-antibody-epr20706-bsa-and-azide-free-ab227891--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img4067.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (AB227891)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | IP | |
|---|---|---|---|
| Mouse | Tested | Tested | Not recommended |
| Rat | Tested | Tested | Not recommended |
| Transfected cell lysate - Mouse | Tested | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Transfected cell lysate - Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Mouse | Dilution info - | Notes - |
IP
Not recommendedNot recommended
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance (PubMed:15339660, PubMed:32528174). Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci) (PubMed:15339660). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance (PubMed:24463520, PubMed:32528174). Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci (PubMed:20733033, PubMed:23650392). Required for the formation of neutrophil extracellular traps (NETs); NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (PubMed:20733033, PubMed:32528174). Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription (By similarity). Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1 (By similarity).
Alternative names
Pad4, Pdi4, Padi4, Protein-arginine deiminase type-4, Peptidylarginine deiminase IV, Protein-arginine deiminase type IV
Recommended products
Rabbit Recombinant Monoclonal PADI4 / PAD4 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Transfected cell lysate - Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20706
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab227891 is the carrier-free version of Anti-PADI4 / PAD4 antibody [EPR20706] ab214810.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PADI4 also known as PAD4 PADI4 protein or PAD-4 is an enzyme that plays a mechanical role in post-translational modification. It functions by catalyzing the conversion of arginine residues into citrulline in histones a process known as citrullination or deimination. This enzyme exhibits a molecular mass of approximately 74 kDa and is expressed notably in tissues like bone marrow and sites of inflammation. PADI4 shows high expression in immune cells such as neutrophils which are important for inflammatory responses.
- Biological function summary
The PADI4 protein impacts gene expression by modifying chromatin architecture making it part of chromatin remodeling activities. PADI4 is known to associate with chromatin but it does not form large stable complexes. It influences the transcriptional activity of genes by altering histone interactions which can affect immune responses and cellular differentiation. Through these chromatin changes PADI4 plays a notable role in regulating genes associated with immune function and inflammatory responses.
- Pathways
PADI4 integrates into the inflammatory response and apoptosis pathways. It influences apoptosis by modulating pro-inflammatory gene expression and participating in the formation of neutrophil extracellular traps (NETs). PADI4 works with other proteins such as histone H3 and histone H4 to influence immune cell pathways. These interactions underline its role in the regulation of protein expressions during inflammation and immune-mediated processes.
- Associated diseases and disorders
PADI4 connects to rheumatoid arthritis (RA) and certain cancers. In rheumatoid arthritis altered activity of PADI4 can lead to the production of autoantibodies against citrullinated proteins which exacerbates inflammation in joints. The enzyme’s dysregulation is also implicated in cancer progression where it may affect tumor growth and metastasis. PADI4’s interaction with other proteins like citrullinated histones highlights its association with pathogenic processes in such diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891), expandable thumbnail](https://content.abcam.com/products/images/padi4-pad4-antibody-epr20706-bsa-and-azide-free-ab227891--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img71801.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891)
This data was developed using Anti-PADI4 / PAD4 antibody [EPR20706] ab214810, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling PADI4 / PAD4 with Anti-PADI4 / PAD4 antibody [EPR20706] ab214810 at 1/100 (11.55 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on granulocytes in rat lung.The section was incubated with Anti-PADI4 / PAD4 antibody [EPR20706] ab214810 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891), expandable thumbnail](https://content.abcam.com/products/images/padi4-pad4-antibody-epr20706-bsa-and-azide-free-ab227891--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102642.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891)
This data was developed using Anti-PADI4 / PAD4 antibody [EPR20706] ab214810, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling PADI4 / PAD4 with Anti-PADI4 / PAD4 antibody [EPR20706] ab214810 at 1/100 (11.55 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on granulocytes in mouse spleen.The section was incubated with Anti-PADI4 / PAD4 antibody [EPR20706] ab214810 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891), expandable thumbnail](https://content.abcam.com/products/images/padi4-pad4-antibody-epr20706-bsa-and-azide-free-ab227891--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img66846.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891)
This data was developed using Anti-PADI4 / PAD4 antibody [EPR20706] ab214810, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling PADI4 / PAD4 with Anti-PADI4 / PAD4 antibody [EPR20706] ab214810 at 1/100 (11.55 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on granulocytes in mouse lung.The section was incubated with Anti-PADI4 / PAD4 antibody [EPR20706] ab214810 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891), expandable thumbnail](https://content.abcam.com/products/images/padi4-pad4-antibody-epr20706-bsa-and-azide-free-ab227891--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img4067.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [EPR20706] - BSA and Azide free (ab227891)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling PADI4 / PAD4 with Anti-PADI4 / PAD4 antibody [EPR20706] ab214810 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Strongly nuclear staining on peripheral blood granulocytes of mouse spleen (PMID: 20733033, PMID: 28281906, PMID: 12393868, PMID: 12833157). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PADI4 / PAD4 antibody [EPR20706] ab214810).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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