AB128086

Anti-PADI4 / PAD4 antibody [OTI4H5]

Name: Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086) | Abcam
Brand: Abcam Limited
SKU: AB128086
Price: 390 USD
Availability: InStock
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(1 Review)

(42 Publications)

Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086) is a mouse monoclonal antibody detecting PADI4 / PAD4 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for African green monkey, Human.

- Over 30 publications

View Alternative Names

PAD4, PADI5, PDI5, PADI4, Protein-arginine deiminase type-4, HL-60 PAD, Peptidylarginine deiminase IV, Protein-arginine deiminase type IV

13 Images

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Western blot - Anti-PADI4 / PAD4 antibody [OTI4H5] (AB128086)

HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC206501)

All lanes:

Western blot - Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086) at 1/2000 dilution

Lane 1:

HEK293T cells transfected with pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK293T cells transfected with pCMV6-ENTRY PADI4 / PAD4 cDNA at 5 µg

Predicted band size: 74 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (AB128086)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (AB128086)

ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Human pancreas tissue by Immunohistochemistry.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (AB128086)

ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded carcinoma of Human lung tissue by Immunohistochemistry.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (AB128086)

ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Human prostate tissue by Immunohistochemistry.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (AB128086)

ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Carcinoma of Human prostate tissue by Immunohistochemistry.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (AB128086)

ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Adenocarcinoma of Human endometrium tissue by Immunohistochemistry.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (AB128086)

ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Carcinoma of Human bladder tissue by Immunohistochemistry.

Flow Cytometry (Intracellular) - Anti-PADI4 / PAD4 antibody [OTI4H5] (AB128086)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PADI4 / PAD4 antibody [OTI4H5] (AB128086)

ab128086, at a dilution of 1/100, staining PADI4 / PAD4 in HeLa cells (Red), compared to a nonspecific negative control antibody (Blue), analysed by Flow cytometry (Intracellular).

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PADI4 / PAD4 antibody [OTI4H5] (AB128086)

ab128086, at a dilution of 1/100, staining PADI4 / PAD4 in Jurkat cells (Red), compared to a nonspecific negative control antibody (Blue), analysed by Flow cytometry (Intracellular).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (AB128086)

ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Human lymphoma tissue by Immunohistochemistry.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (AB128086)

ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Adenocarcinoma of Human ovary tissue by Immunohistochemistry.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (AB128086)

ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Human breast tissue by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-PADI4 / PAD4 antibody [OTI4H5] (AB128086)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PADI4 / PAD4 antibody [OTI4H5] (AB128086)

ab128086, at a dilution of 1/100, staining COS7 cells transiently transfected by pCMV6-ENTRY PADI4 / PAD4 by Immunofluorescence.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI4H5

Isotype

IgG2a

Carrier free

Reacts with

Human, African green monkey

Applications

WB, Flow Cyt (Intra), IHC-P, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PADI4.

Q9UM07

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody." }, "African green monkey": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in African green monkey, Human samples.

What is the molecular weight of PADI4 / PAD4?
Anti-PADI4 / PAD4 [OTI4H5] (ab128086) specifically detects a band for PADI4 / PAD4 (UniProt: Q9UM07) at a molecular weight of 74kDa.

Trusted by the scientific community
Anti-PADI4 / PAD4 [OTI4H5] (ab128086) was first used in a scientific publication in 2012 and has been cited over 30 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified from TCS

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PADI4 also known as PAD4 PADI4 protein or PAD-4 is an enzyme that plays a mechanical role in post-translational modification. It functions by catalyzing the conversion of arginine residues into citrulline in histones a process known as citrullination or deimination. This enzyme exhibits a molecular mass of approximately 74 kDa and is expressed notably in tissues like bone marrow and sites of inflammation. PADI4 shows high expression in immune cells such as neutrophils which are important for inflammatory responses.

Biological function summary

The PADI4 protein impacts gene expression by modifying chromatin architecture making it part of chromatin remodeling activities. PADI4 is known to associate with chromatin but it does not form large stable complexes. It influences the transcriptional activity of genes by altering histone interactions which can affect immune responses and cellular differentiation. Through these chromatin changes PADI4 plays a notable role in regulating genes associated with immune function and inflammatory responses.

Pathways

PADI4 integrates into the inflammatory response and apoptosis pathways. It influences apoptosis by modulating pro-inflammatory gene expression and participating in the formation of neutrophil extracellular traps (NETs). PADI4 works with other proteins such as histone H3 and histone H4 to influence immune cell pathways. These interactions underline its role in the regulation of protein expressions during inflammation and immune-mediated processes.

PADI4 connects to rheumatoid arthritis (RA) and certain cancers. In rheumatoid arthritis altered activity of PADI4 can lead to the production of autoantibodies against citrullinated proteins which exacerbates inflammation in joints. The enzyme’s dysregulation is also implicated in cancer progression where it may affect tumor growth and metastasis. PADI4’s interaction with other proteins like citrullinated histones highlights its association with pathogenic processes in such diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance (PubMed : 15339660, PubMed : 15345777, PubMed : 16567635, PubMed : 21245532). Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci) (PubMed : 15339660, PubMed : 15345777, PubMed : 16567635, PubMed : 21245532). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1 : citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance (PubMed : 15339660, PubMed : 15345777, PubMed : 16567635, PubMed : 21245532). Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci (PubMed : 18209087). Required for the formation of neutrophil extracellular traps (NETs); NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription (PubMed : 15345777). Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1 (PubMed : 15731352).

See full target information PADI4

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Publications (42)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 10: PubMed40632882

2025

Gene therapy enhances deoxyribonuclease I treatment in antimyeloperoxidase glomerulonephritis.

Applications

Unspecified application

Species

Unspecified reactive species

Anne Cao Le,Virginie Oudin,Jonathan Dick,Maliha A Alikhan,Timothy A Gottschalk,Lu Lu,Kate E Lawlor,Daniel Koo Yuk Cheong,Mawj Mandwie,Ian E Alexander,A R Kitching,Poh-Yi Gan,Grant J Logan,Kim M O'Sullivan

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 72:387-397 PubMed38752478

2024

DCM-Spheroid Morphs Express PADs and Citrullinated Cytoskeletal Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Alia Sadiq,Justyna Fert-Bober

Molecular cell 84:522-537.e8 PubMed38151017

2023

hRpn13 shapes the proteome and transcriptome through epigenetic factors HDAC8, PADI4, and transcription factor NF-κB p50.

Applications

Unspecified application

Species

Unspecified reactive species

Vasty Osei-Amponsa,Monika Chandravanshi,Xiuxiu Lu,Valentin Magidson,Sudipto Das,Thorkell Andresson,Marzena Dyba,Venkata R Sabbasani,Rolf E Swenson,Caroline Fromont,Biraj Shrestha,Yongmei Zhao,Michelle E Clapp,Raj Chari,Kylie J Walters

PLoS pathogens 19:e1011849 PubMed38055760

2023

Citrullination profile analysis reveals peptidylarginine deaminase 3 as an HSV-1 target to dampen the activity of candidate antiviral restriction factors.

Applications

Unspecified application

Species

Unspecified reactive species

Selina Pasquero,Francesca Gugliesi,Matteo Biolatti,Valentina Dell'Oste,Camilla Albano,Greta Bajetto,Gloria Griffante,Linda Trifirò,Bianca Brugo,Stefano Raviola,Davide Lacarbonara,Qiao Yang,Sen Sudeshna,Leonard Barasa,Hafeez Haniff,Paul R Thompson,Santo Landolfo,Marco De Andrea

Journal of inflammation research 16:5629-5646 PubMed38046404

2023

Neutrophil Extracellular Traps Aggravate Contrast-Induced Acute Kidney Injury by Damaging Glomeruli and Peritubular Capillaries.

Applications

Unspecified application

Species

Unspecified reactive species

Heng Wang,Tingting Gao,Ruijing Zhang,Jie Hu,Siqi Gao,Yuwen Wang,Xiaotong Qi,Yun Zhou,Guoping Zheng,Honglin Dong

Philosophical transactions of the Royal Society of London. Series B, Biological sciences 378:20220477 PubMed37778379

2023

An unbiased proteomic analysis of PAD4 in human monocytes: novel substrates, binding partners and subcellular localizations.

Applications

Unspecified application

Species

Unspecified reactive species

Mekha A Thomas,Seok-Young Kim,Ashley M Curran,Barbara Smith,Brendan Antiochos,Chan Hyun Na,Erika Darrah

Microbiology spectrum :e0408222 PubMed36802172

2023

Plasma Gelsolin Enhances Phagocytosis of Candida auris by Human Neutrophils through Scavenger Receptor Class B.

Applications

Unspecified application

Species

Unspecified reactive species

Łukasz Suprewicz,Karol Skłodowski,Alicja Walewska,Piotr Deptuła,Alicja Sadzyńska,Andrzej Eljaszewicz,Marcin Moniuszko,Paul A Janmey,Robert Bucki

International journal of biological sciences 19:347-361 PubMed36594092

2023

Neutrophil Extracellular Traps Delay Diabetic Wound Healing by Inducing Endothelial-to-Mesenchymal Transition via the Hippo pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shuofei Yang,ShuangShuang Wang,Liang Chen,Zheyu Wang,Jiaquan Chen,Qihong Ni,Xiangjiang Guo,Lan Zhang,Guanhua Xue

International journal of molecular sciences 23: PubMed36362052

2022

Inhibition of Netosis with PAD Inhibitor Attenuates Endotoxin Shock Induced Systemic Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Huanling Yao,Guojie Cao,Zheng Liu,Yue Zhao,Zhanchao Yan,Senzhen Wang,Yuehua Wang,Zhengwei Guo,Yanming Wang

Neoplasia (New York, N.Y.) 33:100835 PubMed36113195

2022

PAD4-dependent citrullination of nuclear translocation of GSK3β promotes colorectal cancer progression via the degradation of nuclear CDKN1A.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaonuan Luo,Shanshan Chang,Siyu Xiao,Yin Peng,Yuli Gao,Fan Hu,Jianxue Liang,Yidan Xu,Kaining Du,Yang Chen,Jiequan Qin,Stephen J Meltzer,Shiqi Deng,Xianling Feng,Xinmin Fan,Gangqiang Hou,Zhe Jin,Xiaojing Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PADI4 / PAD4 antibody [OTI4H5]

Western blot - Anti-PADI4 / PAD4 antibody [OTI4H5] (AB128086)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (AB128086)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (AB128086)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (AB128086)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (AB128086)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (AB128086)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (AB128086)

Flow Cytometry (Intracellular) - Anti-PADI4 / PAD4 antibody [OTI4H5] (AB128086)

Flow Cytometry (Intracellular) - Anti-PADI4 / PAD4 antibody [OTI4H5] (AB128086)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (AB128086)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (AB128086)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PADI4 / PAD4 antibody [OTI4H5] (AB128086)

Immunocytochemistry/ Immunofluorescence - Anti-PADI4 / PAD4 antibody [OTI4H5] (AB128086)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (42)

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