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AB232406

Anti-PAEP/Glycodelin antibody [EP870Y] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal PAEP/Glycodelin antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Glycodelin, GD, Placental protein 14, Pregnancy-associated endometrial alpha-2 globulin, Progestagen-associated endometrial protein, Progesterone-associated endometrial protein, Zona-binding inhibitory factor-1, PP14, PAEG, PEG, ZIF-1, PAEP

2 Images
Western blot - Anti-PAEP/Glycodelin antibody [EP870Y] - BSA and Azide free (AB232406)
  • WB

Unknown

Western blot - Anti-PAEP/Glycodelin antibody [EP870Y] - BSA and Azide free (AB232406)

This data was developed using ab53289, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PAEP/Glycodelin antibody [EP870Y] (<a href='/en-us/products/primary-antibodies/paep-glycodelin-antibody-ep870y-ab53289'>ab53289</a>) at 1/500 dilution

Lane 1:

K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate

Lane 2:

TPA-treated K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

Western blot - Anti-PAEP/Glycodelin antibody [EP870Y] - BSA and Azide free (AB232406)
  • WB

Unknown

Western blot - Anti-PAEP/Glycodelin antibody [EP870Y] - BSA and Azide free (AB232406)

This data was developed using ab53289, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PAEP/Glycodelin antibody [EP870Y] (<a href='/en-us/products/primary-antibodies/paep-glycodelin-antibody-ep870y-ab53289'>ab53289</a>) at 0.3 µg/mL

Lane 1:

Human placenta tissue lysate at 20 µg

Lane 2:

Human kidney tissue lysate at 20 µg

Lane 3:

Human brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 21 kDa

Observed band size: 24 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP870Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab232406 is the carrier-free version of ab53289.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Glycoprotein that regulates critical steps during fertilization and also has immunomonomodulatory effects. Four glycoforms, namely glycodelin-S, -A, -F and -C have been identified in reproductive tissues that differ in glycosylation and biological activity. Glycodelin-A has contraceptive and immunosuppressive activities (PubMed : 7531163, PubMed : 9918684). Glycodelin-C stimulates binding of spermatozoa to the zona pellucida (PubMed : 17192260). Glycodelin-F inhibits spermatozoa-zona pellucida binding and significantly suppresses progesterone-induced acrosome reaction of spermatozoa (PubMed : 12672671). Glycodelin-S in seminal plasma maintains the uncapacitated state of human spermatozoa (PubMed : 15883155).
See full target information Glycodelin
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature 632:157-165 PubMed39020175

2024

Inhibition of IL-11 signalling extends mammalian healthspan and lifespan.

Applications

Unspecified application

Species

Unspecified reactive species

Anissa A Widjaja,Wei-Wen Lim,Sivakumar Viswanathan,Sonia Chothani,Ben Corden,Cibi Mary Dasan,Joyce Wei Ting Goh,Radiance Lim,Brijesh K Singh,Jessie Tan,Chee Jian Pua,Sze Yun Lim,Eleonora Adami,Sebastian Schafer,Benjamin L George,Mark Sweeney,Chen Xie,Madhulika Tripathi,Natalie A Sims,Norbert Hübner,Enrico Petretto,Dominic J Withers,Lena Ho,Jesus Gil,David Carling,Stuart A Cook
View all publications
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Alternative Products

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Anti-PAEP/Glycodelin antibody [13]

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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