Anti-PAEP/Glycodelin antibody [EPR23251-87]

5 product images

• 

  Flow Cytometry (Intracellular) - Anti-PAEP/Glycodelin antibody [EPR23251-87] (ab270454)

  Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized K-562 (Human chronic myelogenous leukemia lymphoblast) cells treated with 2ng/ml TPA for 32h, then together with 300ng/ml BFA for another 16h (Red) / Untreated control (Green) labeling PAEP/Glycodelin with ab270454 at 1/500 dilution (0.1μg) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody. The expression level of PAEP/Glycodelin is induced in TPA treated K-562 cell (PMID:8025274).
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAEP/Glycodelin antibody [EPR23251-87] (ab270454)

  Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling PAEP/Glycodelin with ab270454 at 1/500 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining in glandular epithelial cells of human endometrium is observed. The section was incubated with ab270454 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
  

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

• 

  Western blot - Anti-PAEP/Glycodelin antibody [EPR23251-87] (ab270454)

  Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
  

  Exposure Time: 26 secs.

  All lanes: Western blot - Anti-PAEP/Glycodelin antibody [EPR23251-87] (ab270454) at 1/1000 dilution

  All lanes: Human placenta tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

  Predicted band size: 21 kDa

• 

  Western blot - Anti-PAEP/Glycodelin antibody [EPR23251-87] (ab270454)

  Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
  

  The expression level of PAEP is induced in TPA treated K-562 cell (PMID:8025274).

  Exposure Time: 3 mins.

  All lanes: Western blot - Anti-PAEP/Glycodelin antibody [EPR23251-87] (ab270454) at 1/1000 dilution

  Lane 1: Untreated K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 20 μg

  Lane 2: K-562 treated with 2 ng/ml PMA(TPA) for 2 days whole cell lysate 20 μg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Predicted band size: 21 kDa

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAEP/Glycodelin antibody [EPR23251-87] (ab270454)

  Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissue labelling PAEP/Glycodelin with ab270454 at 1:500 dilution (1.162 μg/ml), followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human endometrial carcinoma is observed. The section was incubated with ab270454 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
  
  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).