Anti-PAEP/Glycodelin antibody [EPR23251-87]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAEP/Glycodelin antibody [EPR23251-87] (AB270454)

Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling PAEP/Glycodelin with ab270454 at 1/500 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in glandular epithelial cells of human endometrium is observed. The section was incubated with ab270454 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-PAEP/Glycodelin antibody [EPR23251-87] (AB270454)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized K-562 (Human chronic myelogenous leukemia lymphoblast) cells treated with 2ng/ml TPA for 32h, then together with 300ng/ml BFA for another 16h (Red) / Untreated control (Green) labeling PAEP/Glycodelin with ab270454 at 1/500 dilution (0.1μg) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. The expression level of PAEP/Glycodelin is induced in TPA treated K-562 cell (PMID : 8025274).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAEP/Glycodelin antibody [EPR23251-87] (AB270454)

Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissue labelling PAEP/Glycodelin with ab270454 at 1 : 500 dilution (1.162 μg/ml), followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human endometrial carcinoma is observed. The section was incubated with ab270454 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

• WB

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Western blot - Anti-PAEP/Glycodelin antibody [EPR23251-87] (AB270454)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST.

The expression level of PAEP is induced in TPA treated K-562 cell (PMID : 8025274).

Exposure Time : 3 mins.

All lanes:

Western blot - Anti-PAEP/Glycodelin antibody [EPR23251-87] (ab270454) at 1/1000 dilution

Lane 1:

Untreated K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 20 μg

Lane 2:

K-562 treated with 2 ng/ml PMA(TPA) for 2 days whole cell lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 21 kDa

false

• WB

Supplier Data

Western blot - Anti-PAEP/Glycodelin antibody [EPR23251-87] (AB270454)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST.

Exposure Time : 26 secs.

All lanes:

Western blot - Anti-PAEP/Glycodelin antibody [EPR23251-87] (ab270454) at 1/1000 dilution

All lanes:

Human placenta tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 21 kDa

false