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AB241288

Anti-PAFAH1B3 antibody

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(2 Publications)

Rabbit Polyclonal PAFAH1B3 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PAFAH1B3 aa 150 to C-terminus.

View Alternative Names

PAFAHG, PAFAH1B3, Platelet-activating factor acetylhydrolase IB subunit alpha1, PAF acetylhydrolase 29 kDa subunit, PAF-AH subunit gamma, PAF-AH 29 kDa subunit, PAFAH subunit gamma

2 Images
Immunoprecipitation - Anti-PAFAH1B3 antibody (AB241288)
  • IP

Supplier Data

Immunoprecipitation - Anti-PAFAH1B3 antibody (AB241288)

PAFAH1B3 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded prepared using NETN lysis buffer) using ab241288 at 6 μg per reaction. Western bot was performed on the immunoprecipitate using ab241288 at 0.4 μg/ml.

Lane 1 : ab241288 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG.

Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-PAFAH1B3 antibody (ab241288)

Predicted band size: 26 kDa

false

Western blot - Anti-PAFAH1B3 antibody (AB241288)
  • WB

Supplier Data

Western blot - Anti-PAFAH1B3 antibody (AB241288)

All lanes:

Western blot - Anti-PAFAH1B3 antibody (ab241288) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg

Predicted band size: 26 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human PAFAH1B3 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15102

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antibody was affinity purified using an epitope specific to PAFAH1B3 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Alpha1 catalytic subunit of the cytosolic type I platelet-activating factor (PAF) acetylhydrolase (PAF-AH (I)) heterotetrameric enzyme that catalyzes the hydrolyze of the acetyl group at the sn-2 position of PAF and its analogs and modulates the action of PAF. The activity and substrate specificity of PAF-AH (I) are affected by its subunit composition. Both alpha1/alpha1 homodimer (PAFAH1B3/PAFAH1B3 homodimer) and alpha1/alpha2 heterodimer(PAFAH1B3/PAFAH1B2 heterodimer) hydrolyze 1-O-alkyl-2-acetyl-sn-glycero-3-phosphoric acid (AAGPA) more efficiently than PAF, but they have little hydrolytic activity towards 1-O-alkyl-2-acetyl-sn-glycero-3-phosphorylethanolamine (AAGPE). Plays an important role during the development of brain.
See full target information PAFAH1B3

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Translational oncology 58:102423 PubMed40414119

2025

GLI2-HRD1 axis facilitates 5-FU resistance in gastric cancer cells by regulating ubiquitination degradation of UCK2.

Applications

Unspecified application

Species

Unspecified reactive species

Chaorong Xue,Xuanzi Zhang,Wanling Yu,Hanbin Lin,Junrong Zhang,Jiawen Liu,Zongqi Weng,Manduo Ouyang,Xinjian Lin

Heliyon 10:e39859 PubMed39553628

2024

Comparative proteomic analysis between tumor tissues and intratumoral exosomes from lung adenocarcinoma patients identifies PAFAH1B3 as an exosomal protein key for initiating metastasis in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Congcong Wang,Ling Xiao,Ling Gao,Jia Wu,Siliang Wang,Miao-Miao Zheng,Chen-Tai Qin,Xian-Ge Huang,Lei Zhou,Wei-Jie Xu,He-Gen Li,Wen-Lian Chen,Li-Hua Zhu,Xing Jin
View all publications

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