Rabbit Recombinant Monoclonal PAFAH1B3 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.
Images
![Western blot - Anti-PAFAH1B3 antibody [EPR11231-82] (AB166906), expandable thumbnail](https://content.abcam.com/products/images/pafah1b3-antibody-epr11231-82-ab166906--western-blot-img143424.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PAFAH1B3 antibody [EPR11231-82] (AB166906)")
![Western blot - Anti-PAFAH1B3 antibody [EPR11231-82] (AB166906), expandable thumbnail](https://content.abcam.com/products/images/pafah1b3-antibody-epr11231-82-ab166906--western-blot-img125268.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PAFAH1B3 antibody [EPR11231-82] (AB166906)")
Publications
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- BMC cancer 22:5252022PAFAH1B3 predicts poor prognosis and promotes progression in lung adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSuping Tang,Jun Ni,Bohua Chen,Fei Sun,Jinbo Huang,Songshi Ni,Zhiyuan TangPubMed 35534807
- OncoTargets and therapy 12:2799-28082019Aberrant expression of PAFAH1B3 associates with poor prognosis and affects proliferation and aggressiveness in hypopharyngeal squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianing Xu et. al.PubMed 31043794
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Predicted | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Predicted | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/5000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Alpha1 catalytic subunit of the cytosolic type I platelet-activating factor (PAF) acetylhydrolase (PAF-AH (I)) heterotetrameric enzyme that catalyzes the hydrolyze of the acetyl group at the sn-2 position of PAF and its analogs and modulates the action of PAF. The activity and substrate specificity of PAF-AH (I) are affected by its subunit composition. Both alpha1/alpha1 homodimer (PAFAH1B3/PAFAH1B3 homodimer) and alpha1/alpha2 heterodimer(PAFAH1B3/PAFAH1B2 heterodimer) hydrolyze 1-O-alkyl-2-acetyl-sn-glycero-3-phosphoric acid (AAGPA) more efficiently than PAF, but they have little hydrolytic activity towards 1-O-alkyl-2-acetyl-sn-glycero-3-phosphorylethanolamine (AAGPE). Plays an important role during the development of brain.
Alternative names
PAFAHG, PAFAH1B3, Platelet-activating factor acetylhydrolase IB subunit alpha1, PAF acetylhydrolase 29 kDa subunit, PAF-AH subunit gamma, PAF-AH 29 kDa subunit, PAFAH subunit gamma
Recommended products
Rabbit Recombinant Monoclonal PAFAH1B3 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR11231-82
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-PAFAH1B3 antibody [EPR11231-82] (ab166906), expandable thumbnail](https://content.abcam.com/products/images/pafah1b3-antibody-epr11231-82-ab166906--western-blot-img143424.jpg "Western blot - Anti-PAFAH1B3 antibody [EPR11231-82] (ab166906)")
Western blot - Anti-PAFAH1B3 antibody [EPR11231-82] (ab166906)
Lanes 1-4: Merged signal (red and green). Green - ab166906 observed at 26 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.ab166906 Anti-PAFAH1B3 antibody [EPR11231-82] was shown to specifically react with PAFAH1B3 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human PAFAH1B3 knockout HEK-293T cell line ab266669 (knockout cell lysate Human PAFAH1B3 knockout HEK-293T cell lysate ab258559) was used. Wild-type and PAFAH1B3 knockout samples were subjected to SDS-PAGE. ab166906 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-PAFAH1B3 antibody [EPR11231-82] (ab166906) at 1/1000 dilution
Lane 1: Wild-type HEK293T cell lysate at 20 µg
Lane 2: PAFAH1B3 knockout HEK293T cell lysate at 20 µg
Lane 2: Western blot - Human PAFAH1B3 knockout HEK-293T cell line (Human PAFAH1B3 knockout HEK-293T cell line ab266669)
Lane 3: K-562 cell lysate at 20 µg
Lane 4: Jurkat cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa
![Western blot - Anti-PAFAH1B3 antibody [EPR11231-82] (ab166906), expandable thumbnail](https://content.abcam.com/products/images/pafah1b3-antibody-epr11231-82-ab166906--western-blot-img125268.jpg "Western blot - Anti-PAFAH1B3 antibody [EPR11231-82] (ab166906)")
Western blot - Anti-PAFAH1B3 antibody [EPR11231-82] (ab166906)
All lanes: Western blot - Anti-PAFAH1B3 antibody [EPR11231-82] (ab166906) at 1/1000 dilution
Lane 1: K562 cell lysate at 10 µg
Lane 2: 293T cell lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
Lane 4: Jurkat cell lysate at 10 µg
Predicted band size: 26 kDa
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