Rabbit Recombinant Monoclonal PAH antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-PAH antibody [EPR12380] - BSA and Azide free (AB202431), expandable thumbnail](https://content.abcam.com/products/images/pah-antibody-epr12380-bsa-and-azide-free-ab202431--immunocytochemistry-immunofluorescence-img241.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PAH antibody [EPR12380] - BSA and Azide free (AB202431)")
![Flow Cytometry (Intracellular) - Anti-PAH antibody [EPR12380] - BSA and Azide free (AB202431), expandable thumbnail](https://content.abcam.com/products/images/pah-antibody-epr12380-bsa-and-azide-free-ab202431--flow-cytometry-intracellular-img43833.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-PAH antibody [EPR12380] - BSA and Azide free (AB202431)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAH antibody [EPR12380] - BSA and Azide free (AB202431), expandable thumbnail](https://content.abcam.com/products/images/pah-antibody-epr12380-bsa-and-azide-free-ab202431--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133097.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAH antibody [EPR12380] - BSA and Azide free (AB202431)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAH antibody [EPR12380] - BSA and Azide free (AB202431), expandable thumbnail](https://content.abcam.com/products/images/pah-antibody-epr12380-bsa-and-azide-free-ab202431--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135744.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAH antibody [EPR12380] - BSA and Azide free (AB202431)")
![Western blot - Anti-PAH antibody [EPR12380] - BSA and Azide free (AB202431), expandable thumbnail](https://content.abcam.com/products/images/pah-antibody-epr12380-bsa-and-azide-free-ab202431--western-blot-img432162.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PAH antibody [EPR12380] - BSA and Azide free (AB202431)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Tested | Tested |
| Mouse | Not recommended | Not recommended | Tested | Expected | Expected |
| Rat | Not recommended | Not recommended | Tested | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
8 products for Alternative Version
Target data
Function
Catalyzes the hydroxylation of L-phenylalanine to L-tyrosine.
Alternative names
Phenylalanine-4-hydroxylase, PAH, Phe-4-monooxygenase
Recommended products
Rabbit Recombinant Monoclonal PAH antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR12380
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab202431 is the carrier-free version of Anti-PAH antibody [EPR12380] ab178430.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunocytochemistry/ Immunofluorescence - Anti-PAH antibody [EPR12380] - BSA and Azide free (ab202431), expandable thumbnail](https://content.abcam.com/products/images/pah-antibody-epr12380-bsa-and-azide-free-ab202431--immunocytochemistry-immunofluorescence-img241.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PAH antibody [EPR12380] - BSA and Azide free (ab202431)")
Immunocytochemistry/ Immunofluorescence - Anti-PAH antibody [EPR12380] - BSA and Azide free (ab202431)
Immunofluorescent analysis of HepG2 cells labeling PAH with Anti-PAH antibody [EPR12380] ab178430 at 1/50 dilution (green). DAPI nuclear staining (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAH antibody [EPR12380] ab178430).
![Flow Cytometry (Intracellular) - Anti-PAH antibody [EPR12380] - BSA and Azide free (ab202431), expandable thumbnail](https://content.abcam.com/products/images/pah-antibody-epr12380-bsa-and-azide-free-ab202431--flow-cytometry-intracellular-img43833.jpg "Flow Cytometry (Intracellular) - Anti-PAH antibody [EPR12380] - BSA and Azide free (ab202431)")
Flow Cytometry (Intracellular) - Anti-PAH antibody [EPR12380] - BSA and Azide free (ab202431)
Intracellular flow cytometric analysis of permeabilized HepG2 cells labeling PAH with Anti-PAH antibody [EPR12380] ab178430 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAH antibody [EPR12380] ab178430).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAH antibody [EPR12380] - BSA and Azide free (ab202431), expandable thumbnail](https://content.abcam.com/products/images/pah-antibody-epr12380-bsa-and-azide-free-ab202431--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133097.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAH antibody [EPR12380] - BSA and Azide free (ab202431)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAH antibody [EPR12380] - BSA and Azide free (ab202431)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling PAH with Anti-PAH antibody [EPR12380] ab178430 at 1/50 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAH antibody [EPR12380] ab178430).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAH antibody [EPR12380] - BSA and Azide free (ab202431), expandable thumbnail](https://content.abcam.com/products/images/pah-antibody-epr12380-bsa-and-azide-free-ab202431--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135744.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAH antibody [EPR12380] - BSA and Azide free (ab202431)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAH antibody [EPR12380] - BSA and Azide free (ab202431)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PAH with Anti-PAH antibody [EPR12380] ab178430 at 1/50 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAH antibody [EPR12380] ab178430).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Western blot - Anti-PAH antibody [EPR12380] - BSA and Azide free (ab202431), expandable thumbnail](https://content.abcam.com/products/images/pah-antibody-epr12380-bsa-and-azide-free-ab202431--western-blot-img432162.jpg "Western blot - Anti-PAH antibody [EPR12380] - BSA and Azide free (ab202431)")
Western blot - Anti-PAH antibody [EPR12380] - BSA and Azide free (ab202431)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAH antibody [EPR12380] ab178430).
Blocking and dilution buffer: 5% NFDM/TBST
Negative control: mouse spleen.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
All lanes: Western blot - Anti-PAH antibody [EPR12380] (Anti-PAH antibody [EPR12380] ab178430)
Lane 1: Mouse liver tissue lysate at 20 µg
Lane 2: Mouse spleen tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa
![Western blot - Anti-PAH antibody [EPR12380] - BSA and Azide free (ab202431), expandable thumbnail](https://content.abcam.com/products/images/pah-antibody-epr12380-bsa-and-azide-free-ab202431--western-blot-img432163.jpg "Western blot - Anti-PAH antibody [EPR12380] - BSA and Azide free (ab202431)")
Western blot - Anti-PAH antibody [EPR12380] - BSA and Azide free (ab202431)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAH antibody [EPR12380] ab178430).
Blocking and dilution buffer: 5% NFDM/TBST.
Negative control: rat spleen.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
All lanes: Western blot - Anti-PAH antibody [EPR12380] (Anti-PAH antibody [EPR12380] ab178430) at 1/500 dilution
Lane 1: Rat liver tissue lysate at 20 µg
Lane 2: Rat spleen tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa
![Western blot - Anti-PAH antibody [EPR12380] - BSA and Azide free (ab202431), expandable thumbnail](https://content.abcam.com/products/images/pah-antibody-epr12380-bsa-and-azide-free-ab202431--western-blot-img402437.jpg "Western blot - Anti-PAH antibody [EPR12380] - BSA and Azide free (ab202431)")
Western blot - Anti-PAH antibody [EPR12380] - BSA and Azide free (ab202431)
All lanes: Western blot - Anti-PAH antibody [EPR12380] (Anti-PAH antibody [EPR12380] ab178430) at 1/10000 dilution
Lane 1: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: U-87MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 51 kDa
Exposure time: 180s
![Western blot - Anti-PAH antibody [EPR12380] - BSA and Azide free (ab202431), expandable thumbnail](https://content.abcam.com/products/images/pah-antibody-epr12380-bsa-and-azide-free-ab202431--western-blot-img432163.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PAH antibody [EPR12380] - BSA and Azide free (ab202431)")
![Western blot - Anti-PAH antibody [EPR12380] - BSA and Azide free (ab202431), expandable thumbnail](https://content.abcam.com/products/images/pah-antibody-epr12380-bsa-and-azide-free-ab202431--western-blot-img402437.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PAH antibody [EPR12380] - BSA and Azide free (ab202431)")
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