AB125687

Anti-PAI1 antibody [1D5]

Name: Anti-PAI1 antibody [1D5] (ab125687) | Abcam
Brand: Abcam Limited
SKU: AB125687
Price: 545 USD
Availability: InStock

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(19 Publications)

Mouse Monoclonal PAI1 antibody. Suitable for Flow Cyt, ELISA, IHC-P and reacts with Mouse, Human, Synthetic peptide samples. Cited in 19 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SERPINE1.

View Alternative Names

PAI1, PLANH1, SERPINE1, Plasminogen activator inhibitor 1, PAI, PAI-1, Endothelial plasminogen activator inhibitor, Serpin E1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody [1D5] (AB125687)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody [1D5] (AB125687)

ab123687 at 1/200 dilution staining PAI1 in Paraffin-embedded Human lung cancer tissue by Immunohistochemistry followed by DAB staining.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody [1D5] (AB125687)

ab123687 at 1/200 dilution staining PAI1 in Paraffin-embedded Human kidney cancer tissue by Immunohistochemistry followed by DAB staining.

Flow Cytometry - Anti-PAI1 antibody [1D5] (AB125687)

Flow Cyt

Unknown

Flow Cytometry - Anti-PAI1 antibody [1D5] (AB125687)

ab125687 at 1/200 dilution staining PAI1 in NIH 3T3 cells by Flow Cytometry (green), negative control (red).

ELISA - Anti-PAI1 antibody [1D5] (AB125687)

ELISA

Unknown

ELISA - Anti-PAI1 antibody [1D5] (AB125687)

ELISA using ab125687.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1D5

Isotype

IgG1

Carrier free

Reacts with

Mouse, Human

Applications

ELISA, Flow Cyt, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SERPINE1. The exact immunogen used to generate this antibody is proprietary information.

P05121

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Synthetic peptide": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1/10000", "ELISA-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

This product was changed from ascites to supernatant. Lot no's high than GR201079-27 are from Tissue Culture Supernatant

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine protease inhibitor. Inhibits TMPRSS7 (PubMed : 15853774). Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots (PubMed : 17912461, PubMed : 8481516, PubMed : 9207454, PubMed : 21925150). As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading (PubMed : 9175705). Acts as a regulator of cell migration, independently of its role as protease inhibitor (PubMed : 15001579, PubMed : 9168821). It is required for stimulation of keratinocyte migration during cutaneous injury repair (PubMed : 18386027). It is involved in cellular and replicative senescence (PubMed : 16862142). Plays a role in alveolar type 2 cells senescence in the lung (By similarity). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (PubMed : 25808697, PubMed : 27046084).

See full target information SERPINE1

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 44:90 PubMed40059196

2025

NONO interacts with nuclear PKM2 and directs histone H3 phosphorylation to promote triple-negative breast cancer metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Qixiang Li,Hongfei Ci,Pengpeng Zhao,Dongjun Yang,Yi Zou,Panhai Chen,Dongliang Wu,Wenbing Shangguan,Wenyang Li,Xingjun Meng,Mengying Xing,Yuzhong Chen,Ming Zhang,Bing Chen,Lingdong Kong,Ke Zen,David C S Huang,Zhi-Wei Jiang,Quan Zhao

Journal of Cancer 14:114-128 PubMed36605486

2023

Anti-PAI-1 Monoclonal Antibody Inhibits the Metastasis and Growth of Esophageal Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yutian Zheng,Lin Meng,Like Qu,Chuanke Zhao,Lixin Wang,Caiyun Liu,Chengchao Shou

Frontiers in surgery 9:819018 PubMed35372476

2022

The Prognostic Significance and Gene Expression Characteristics of Gastric Signet-Ring Cell Carcinoma: A Study Based on the SEER and TCGA Databases.

Applications

Unspecified application

Species

Unspecified reactive species

Junren Ma,Yan Meng,Xin Zhou,Limei Guo,Wei Fu

Oncology reports 47: PubMed35059742

2022

Hypoxia induces radioresistance, epithelial‑mesenchymal transition, cancer stem cell‑like phenotype and changes in genes possessing multiple biological functions in head and neck squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Emilia Wiechec,Natasa Matic,Ashfaq Ali,Karin Roberg

Cells 10: PubMed34943956

2021

Primary Human Trabecular Meshwork Model for Pseudoexfoliation.

Applications

Unspecified application

Species

Unspecified reactive species

Munmun Chakraborty,Prity Sahay,Aparna Rao

Scientific reports 11:22805 PubMed34815441

2021

Assessment of histological characteristics, imaging markers, and rt-PA susceptibility of ex vivo venous thrombi.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel A Hendley,Alexey Dimov,Aarushi Bhargava,Erin Snoddy,Daniel Mansour,Rana O Afifi,Geoffrey D Wool,Yuanyuan Zha,Steffen Sammet,Zheng Feng Lu,Osman Ahmed,Jonathan D Paul,Kenneth B Bader

International journal of oncology 58:111-121 PubMed33367928

2020

Crosstalk between YAP and TGFβ regulates SERPINE1 expression in mesenchymal lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hyeon-Joon Kong,Eun-Ji Kwon,Ok-Seon Kwon,Haeseung Lee,Jeong-Yun Choi,Yung-Jeong Kim,Wankyu Kim,Hyuk-Jin Cha

Blood 134:2399-2413 PubMed31877217

2019

Metabolites in a mouse cancer model enhance venous thrombogenicity through the aryl hydrocarbon receptor-tissue factor axis.

Applications

Unspecified application

Species

Unspecified reactive species

Mostafa Belghasem,Daniel Roth,Sean Richards,Marc Arthur Napolene,Joshua Walker,Wenqing Yin,Nkiruka Arinze,Chimera Lyle,Cheryl Spencer,Jean M Francis,Cristal Thompson,Christopher Andry,Stephen A Whelan,Norman Lee,Katya Ravid,Vipul C Chitalia

Journal of cellular and molecular medicine 23:4179-4195 PubMed30955247

2019

PTPN2 improved renal injury and fibrosis by suppressing STAT-induced inflammation in early diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Ya Li,Huimin Zhou,Yulin Li,Lu Han,Ming Song,Fangfang Chen,Guokai Shang,Di Wang,Zhihao Wang,Wei Zhang,Ming Zhong

Journal of cellular physiology 234:6898-6907 PubMed30317577

2018

Effects of microRNA-370 on mesangial cell proliferation and extracellular matrix accumulation by binding to canopy 1 in a rat model of diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Feng-Na Yu,Ming-Lan Hu,Xin-Fang Wang,Xiang-Ping Li,Bei-Hao Zhang,Xiao-Qing Lu,Rui-Qiang Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PAI1 antibody [1D5]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody [1D5] (AB125687)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody [1D5] (AB125687)

Flow Cytometry - Anti-PAI1 antibody [1D5] (AB125687)

ELISA - Anti-PAI1 antibody [1D5] (AB125687)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (19)

Product promise