Mouse Monoclonal PAI1 antibody. Suitable for Flow Cyt, ELISA, IHC-P and reacts with Mouse, Human, Synthetic peptide samples. Cited in 17 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SERPINE1.
Images
![ELISA - Anti-PAI1 antibody [1D5] (AB125687), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-1d5-ab125687--elisa-img136542.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Anti-PAI1 antibody [1D5] (AB125687)")
![Flow Cytometry - Anti-PAI1 antibody [1D5] (AB125687), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-1d5-ab125687--flow-cytometry-img75766.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-PAI1 antibody [1D5] (AB125687)")
Publications
Filter by
- Journal of Cancer 14:114-1282023Anti-PAI-1 Monoclonal Antibody Inhibits the Metastasis and Growth of Esophageal Squamous Cell Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYutian Zheng,Lin Meng,Like Qu,Chuanke Zhao,Lixin Wang,Caiyun Liu,Chengchao ShouPubMed 36605486
- Frontiers in surgery 9:8190182022The Prognostic Significance and Gene Expression Characteristics of Gastric Signet-Ring Cell Carcinoma: A Study Based on the SEER and TCGA Databases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunren Ma,Yan Meng,Xin Zhou,Limei Guo,Wei FuPubMed 35372476
- Oncology reports 47:2022Hypoxia induces radioresistance, epithelial‑mesenchymal transition, cancer stem cell‑like phenotype and changes in genes possessing multiple biological functions in head and neck squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmilia Wiechec,Natasa Matic,Ashfaq Ali,Karin RobergPubMed 35059742
- Cells 10:2021Primary Human Trabecular Meshwork Model for Pseudoexfoliation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMunmun Chakraborty,Prity Sahay,Aparna RaoPubMed 34943956
- Scientific reports 11:228052021Assessment of histological characteristics, imaging markers, and rt-PA susceptibility of ex vivo venous thrombi.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSamuel A Hendley,Alexey Dimov,Aarushi Bhargava,Erin Snoddy,Daniel Mansour,Rana O Afifi,Geoffrey D Wool,Yuanyuan Zha,Steffen Sammet,Zheng Feng Lu,Osman Ahmed,Jonathan D Paul,Kenneth B BaderPubMed 34815441
- International journal of oncology 58:111-1212020Crosstalk between YAP and TGFβ regulates SERPINE1 expression in mesenchymal lung cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyeon-Joon Kong,Eun-Ji Kwon,Ok-Seon Kwon,Haeseung Lee,Jeong-Yun Choi,Yung-Jeong Kim,Wankyu Kim,Hyuk-Jin ChaPubMed 33367928
- Blood 134:2399-24132019Metabolites in a mouse cancer model enhance venous thrombogenicity through the aryl hydrocarbon receptor-tissue factor axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMostafa Belghasem,Daniel Roth,Sean Richards,Marc Arthur Napolene,Joshua Walker,Wenqing Yin,Nkiruka Arinze,Chimera Lyle,Cheryl Spencer,Jean M Francis,Cristal Thompson,Christopher Andry,Stephen A Whelan,Norman Lee,Katya Ravid,Vipul C ChitaliaPubMed 31877217
- Journal of cellular and molecular medicine 23:4179-41952019PTPN2 improved renal injury and fibrosis by suppressing STAT-induced inflammation in early diabetic nephropathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYa Li et. al.PubMed 30955247
- Journal of oral pathology & medicine : official pu 47:566-5742018PAI-1, CAIX, and VEGFA expressions as prognosis markers in oral squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGabriela Tonini Peterle et. al.PubMed 29693741
- Cell proliferation 51:e124622018Repression of microRNA-382 inhibits glomerular mesangial cell proliferation and extracellular matrix accumulation via FoxO1 in mice with diabetic nephropathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShan Wang et. al.PubMed 29701296
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Fragment Protein within Human SERPINE1. The exact immunogen used to generate this antibody is proprietary information. Database link P05121
Reactivity data
Select an application| Flow Cyt | ELISA | IHC-P | |
|---|---|---|---|
| Human | Expected | Expected | Tested |
| Mouse | Tested | Expected | Expected |
| Synthetic peptide | Not recommended | Tested | Not recommended |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/200.00000 - 1/400.00000 | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info 1/10000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Serine protease inhibitor. Inhibits TMPRSS7 (PubMed:15853774). Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots (PubMed:8481516, PubMed:9207454, PubMed:17912461). As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading (PubMed:9175705). Acts as a regulator of cell migration, independently of its role as protease inhibitor (PubMed:15001579, PubMed:9168821). It is required for stimulation of keratinocyte migration during cutaneous injury repair (PubMed:18386027). It is involved in cellular and replicative senescence (PubMed:16862142). Plays a role in alveolar type 2 cells senescence in the lung (By similarity). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (PubMed:25808697, PubMed:27046084).
Alternative names
Plasminogen activator inhibitor 1, PAI, PAI-1, Endothelial plasminogen activator inhibitor, Serpin E1, PLANH1, PAI1, SERPINE1
Recommended products
Mouse Monoclonal PAI1 antibody. Suitable for Flow Cyt, ELISA, IHC-P and reacts with Mouse, Human, Synthetic peptide samples. Cited in 17 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SERPINE1.
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human SERPINE1. The exact immunogen used to generate this antibody is proprietary information. Database link P05121
- Clone number
1D5
- Purification technique
Affinity purification Protein G
- Concentration
- Purification notes
Purified from tissue culture supernatant.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
This product was changed from ascites to supernatant. Lot no's high than GR201079-27 are from Tissue Culture Supernatant
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![ELISA - Anti-PAI1 antibody [1D5] (ab125687), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-1d5-ab125687--elisa-img136542.jpg "ELISA - Anti-PAI1 antibody [1D5] (ab125687)")
ELISA - Anti-PAI1 antibody [1D5] (ab125687)
ELISA using ab125687.
![Flow Cytometry - Anti-PAI1 antibody [1D5] (ab125687), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-1d5-ab125687--flow-cytometry-img75766.jpg "Flow Cytometry - Anti-PAI1 antibody [1D5] (ab125687)")
Flow Cytometry - Anti-PAI1 antibody [1D5] (ab125687)
ab125687 at 1/200 dilution staining PAI1 in NIH 3T3 cells by Flow Cytometry (green), negative control (red).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody [1D5] (ab125687), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-1d5-ab125687--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114530.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody [1D5] (ab125687)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody [1D5] (ab125687)
ab123687 at 1/200 dilution staining PAI1 in Paraffin-embedded Human lung cancer tissue by Immunohistochemistry followed by DAB staining.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody [1D5] (ab125687), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-1d5-ab125687--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img32631.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody [1D5] (ab125687)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody [1D5] (ab125687)
ab123687 at 1/200 dilution staining PAI1 in Paraffin-embedded Human kidney cancer tissue by Immunohistochemistry followed by DAB staining.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com