AB31280

Anti-PAI1 antibody

Name: Anti-PAI1 antibody (ab31280) | Abcam
Brand: Abcam Limited
SKU: AB31280
Rating: 5 (4 reviews)

(4 Reviews)

(9 Publications)

Goat Polyclonal PAI1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human SERPINE1 aa 50-100.

View Alternative Names

PAI1, PLANH1, SERPINE1, Plasminogen activator inhibitor 1, PAI, PAI-1, Endothelial plasminogen activator inhibitor, Serpin E1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody (AB31280)

IHC-P

AbReview15102****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody (AB31280)

ab31280, at 1/1000, staining plasminogen activator inhibitor 1 (PAI1) in human MDA-MB-231 cells grown in mice by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed and blocked in 10% casein for 30 minutes at 22°C and then incubated with ab31280, for 17 hours at 4°C. Horse polyclonal to goat IgG, diluted 1/200, was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody (AB31280)

ab31280 (4µg/ml) staining of paraffin embedded Human Placenta shows strong staining of cytotrophoblasts. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Supplier Data

Western blot - Anti-PAI1 antibody (AB31280)

All lanes:

Western blot - Anti-PAI1 antibody (ab31280) at 0.1 µg/mL

Lane 1:

A549 cell lysate (35µg protein in RIPA buffer)

Lane 2:

HepG2 cell lysate (35µg protein in RIPA buffer)

Lane 3:

HeLa cell lysate (35µg protein in RIPA buffer)

Predicted band size: 45 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human SERPINE1 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

P05121

Specificity

This antibody is expected to recognise both reported isoforms.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "4-6 µg/mL", "IHCP-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine protease inhibitor. Inhibits TMPRSS7 (PubMed : 15853774). Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots (PubMed : 17912461, PubMed : 8481516, PubMed : 9207454, PubMed : 21925150). As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading (PubMed : 9175705). Acts as a regulator of cell migration, independently of its role as protease inhibitor (PubMed : 15001579, PubMed : 9168821). It is required for stimulation of keratinocyte migration during cutaneous injury repair (PubMed : 18386027). It is involved in cellular and replicative senescence (PubMed : 16862142). Plays a role in alveolar type 2 cells senescence in the lung (By similarity). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (PubMed : 25808697, PubMed : 27046084).

See full target information SERPINE1

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Cancer genomics & proteomics 22:13-23 PubMed39730177

2024

G Protein-coupled Estrogen Receptor 1 (GPER1) Regulates Expression of /PAI-1 and Inhibits Tumorigenic Potential of Cervical Squamous Cell Carcinoma Cells .

Applications

Unspecified application

Species

Unspecified reactive species

Linea Rörig,Sophia Ruckriegl,Julia Gallwas,Carsten Gründker

iScience 25:104022 PubMed35330685

2022

Caveolin-1 peptide regulates p53-microRNA-34a feedback in fibrotic lung fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Taryn B Hogan,Nivedita Tiwari,M R Nagaraja,Shwetha K Shetty,Liang Fan,Rashmi S Shetty,Yashodhar P Bhandary,Sreerama Shetty

Life sciences 239:117092 PubMed31760103

2019

Circulating miR-30c as a predictive biomarker of type 2 diabetes mellitus with coronary heart disease by regulating PAI-1/VN interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Mao Luo,Gang Wang,Chunrong Xu,Min Zeng,Fang Lin,Jianbo Wu,Qin Wan

Biology open 8: PubMed31649116

2019

MiR145-5p inhibits proliferation of PMVECs via PAI-1 in experimental hepatopulmonary syndrome rat pulmonary microvascular hyperplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Chen,Congwen Yang,Yujie Li,Lin Chen,Yong Yang,Karine Belguise,Xiaobo Wang,Kaizhi Lu,Bin Yi

International journal of molecular sciences 19: PubMed29510576

2018

SK-216, a Novel Inhibitor of Plasminogen Activator Inhibitor-1, Suppresses Lung Metastasis of Human Osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Minori Tsuge,Mitsuhiko Osaki,Ryo Sasaki,Mio Hirahata,Futoshi Okada

Cancer medicine 5:892-902 PubMed26817521

2016

PAI-1, a target gene of miR-143, regulates invasion and metastasis by upregulating MMP-13 expression of human osteosarcoma.

Applications

Species

Unspecified reactive species

Mio Hirahata,Mitsuhiko Osaki,Yusuke Kanda,Yui Sugimoto,Yusuke Yoshioka,Nobuyoshi Kosaka,Fumitaka Takeshita,Tomohiro Fujiwara,Akira Kawai,Hisao Ito,Takahiro Ochiya,Futoshi Okada

Oncotarget 6:8103-14 PubMed25797271

2015

Secreted uPAR isoform 2 (uPAR7b) is a novel direct target of miR-221.

Applications

Species

Unspecified reactive species

Natalie Falkenberg,Nataša Anastasov,Annalisa Schaub,Vanja Radulovic,Manfred Schmitt,Viktor Magdolen,Michaela Aubele

Journal of Crohn's & colitis 7:403-11 PubMed22921465

2012

Plasminogen activator inhibitor-1 is increased in colonic epithelial cells from patients with colitis-associated cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Earl Gillespie,Susan E Leeman,Luisa A Watts,Jennifer A Coukos,Michael J O'Brien,Sandra R Cerda,Francis A Farraye,Arthur F Stucchi

Infection and immunity 76:3992-9 PubMed18519558

2008

Helicobacter pylori infection stimulates plasminogen activator inhibitor 1 production by gastric epithelial cells.

Applications

Species

Human

A C Keates,S Tummala,R M Peek,E Csizmadia,B Kunzli,K Becker,P Correa,J Romero-Gallo,M B Piazuelo,S Sheth,C P Kelly,S C Robson,S Keates

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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