Goat Polyclonal PAI1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human SERPINE1 aa 50-100.
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Publications
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- iScience 25:1040222022Caveolin-1 peptide regulates p53-microRNA-34a feedback in fibrotic lung fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTaryn B Hogan,Nivedita Tiwari,M R Nagaraja,Shwetha K Shetty,Liang Fan,Rashmi S Shetty,Yashodhar P Bhandary,Sreerama ShettyPubMed 35330685
- Life sciences 239:1170922019Circulating miR-30c as a predictive biomarker of type 2 diabetes mellitus with coronary heart disease by regulating PAI-1/VN interactions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMao Luo et. al.PubMed 31760103
- Biology open 8:2019MiR145-5p inhibits proliferation of PMVECs via PAI-1 in experimental hepatopulmonary syndrome rat pulmonary microvascular hyperplasia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Chen et. al.PubMed 31649116
- International journal of molecular sciences 19:2018SK-216, a Novel Inhibitor of Plasminogen Activator Inhibitor-1, Suppresses Lung Metastasis of Human Osteosarcoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMinori Tsuge et. al.PubMed 29510576
- Cancer medicine 5:892-9022016PAI-1, a target gene of miR-143, regulates invasion and metastasis by upregulating MMP-13 expression of human osteosarcoma.
- Oncotarget 6:8103-142015Secreted uPAR isoform 2 (uPAR7b) is a novel direct target of miR-221.Applications:WBReactive species:Unspecified reactive speciesNatalie Falkenberg,Nataša Anastasov,Annalisa Schaub,Vanja Radulovic,Manfred Schmitt,Viktor Magdolen,Michaela AubelePubMed 25797271
- Journal of Crohn's & colitis 7:403-112012Plasminogen activator inhibitor-1 is increased in colonic epithelial cells from patients with colitis-associated cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEarl Gillespie,Susan E Leeman,Luisa A Watts,Jennifer A Coukos,Michael J O'Brien,Sandra R Cerda,Francis A Farraye,Arthur F StucchiPubMed 22921465
- Infection and immunity 76:3992-92008Helicobacter pylori infection stimulates plasminogen activator inhibitor 1 production by gastric epithelial cells.
Key facts
- Isotype
IgG
- Host species
Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human SERPINE1 aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link P05121
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Chimpanzee | Predicted | Predicted |
| Dog | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.10000-0.30000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog, Chimpanzee | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 4.00000-6.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog, Chimpanzee | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Serine protease inhibitor. Inhibits TMPRSS7 (PubMed:15853774). Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots (PubMed:17912461, PubMed:8481516, PubMed:9207454). As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading (PubMed:9175705). Acts as a regulator of cell migration, independently of its role as protease inhibitor (PubMed:15001579, PubMed:9168821). It is required for stimulation of keratinocyte migration during cutaneous injury repair (PubMed:18386027). It is involved in cellular and replicative senescence (PubMed:16862142). Plays a role in alveolar type 2 cells senescence in the lung (By similarity). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (PubMed:25808697, PubMed:27046084).
Alternative names
PAI1, PLANH1, PLANH1, PAI1, SERPINE1, Plasminogen activator inhibitor 1, PAI, PAI-1, Endothelial plasminogen activator inhibitor, Serpin E1
Recommended products
Goat Polyclonal PAI1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human SERPINE1 aa 50-100.
Key facts
- Isotype
IgG
- Host species
Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human SERPINE1 aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link P05121
- Purification technique
Affinity purification Immunogen
- Specificity
This antibody is expected to recognise both reported isoforms.
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Anti-PAI1 antibody (ab31280)
All lanes: Western blot - Anti-PAI1 antibody (ab31280) at 0.1 µg/mL
Lane 1: A549 cell lysate (35µg protein in RIPA buffer)
Lane 2: HepG2 cell lysate (35µg protein in RIPA buffer)
Lane 3: HeLa cell lysate (35µg protein in RIPA buffer)
Developed using the ECL technique.
Predicted band size: 45 kDa
This image is courtesy of an anonymous customer reviewImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody (ab31280)
ab31280, at 1/1000, staining plasminogen activator inhibitor 1 (PAI1) in human MDA-MB-231 cells grown in mice by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed and blocked in 10% casein for 30 minutes at 22°C and then incubated with ab31280, for 17 hours at 4°C. Horse polyclonal to goat IgG, diluted 1/200, was used as the secondary antibody.
Western blot - Anti-PAI1 antibody (ab31280)
All lanes: Western blot - Anti-PAI1 antibody (ab31280) at 0.3 µg/mL
All lanes: Human liver lysate (35µg protein in RIPA buffer)
Developed using the ECL technique.
Predicted band size: 45 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody (ab31280)
ab31280 (4µg/ml) staining of paraffin embedded Human Placenta shows strong staining of cytotrophoblasts. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
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