Rabbit Recombinant Monoclonal PAI1 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IP and reacts with Mouse, Rat, Human samples.
Images
![Western blot - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (AB226864), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-epr17272-21-bsa-and-azide-free-ab226864--western-blot-img50882.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (AB226864)")
![Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (AB226864), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-epr17272-21-bsa-and-azide-free-ab226864--immunocytochemistry-immunofluorescence-img108093.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (AB226864)")
![Flow Cytometry (Intracellular) - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (AB226864), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-epr17272-21-bsa-and-azide-free-ab226864--flow-cytometry-intracellular-img140945.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (AB226864)")
![Western blot - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (AB226864), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-epr17272-21-bsa-and-azide-free-ab226864--western-blot-img54559.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (AB226864)")
![Western blot - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (AB226864), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-epr17272-21-bsa-and-azide-free-ab226864--western-blot-img30849.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (AB226864)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | IP | |
|---|---|---|---|---|
| Human | Tested | Expected | Expected | Tested |
| Mouse | Tested | Tested | Tested | Tested |
| Rat | Tested | Expected | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Serine protease inhibitor. Inhibits TMPRSS7 (PubMed:15853774). Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots (PubMed:17912461, PubMed:8481516, PubMed:9207454). As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading (PubMed:9175705). Acts as a regulator of cell migration, independently of its role as protease inhibitor (PubMed:15001579, PubMed:9168821). It is required for stimulation of keratinocyte migration during cutaneous injury repair (PubMed:18386027). It is involved in cellular and replicative senescence (PubMed:16862142). Plays a role in alveolar type 2 cells senescence in the lung (By similarity). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (PubMed:25808697, PubMed:27046084).
Alternative names
PAI1, PLANH1, PLANH1, PAI1, SERPINE1, Plasminogen activator inhibitor 1, PAI, PAI-1, Endothelial plasminogen activator inhibitor, Serpin E1
Recommended products
Rabbit Recombinant Monoclonal PAI1 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IP and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR17272-21
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab226864 is the carrier-free version of Anti-PAI1 antibody [EPR17272-21] ab182973.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-epr17272-21-bsa-and-azide-free-ab226864--western-blot-img50882.jpg "Western blot - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864)")
Western blot - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864)
This data was developed using the same antibody clone in a different buffer formulation (Anti-PAI1 antibody [EPR17272-21] ab182973).
Lanes 1 - 3: Merged signal (red and green). Green - Anti-PAI1 antibody [EPR17272-21] ab182973 observed at 48 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
Anti-PAI1 antibody [EPR17272-21] ab182973 was shown to react with PAI1 in wild-type A549 cells in Western blot. The band observed in the edited lysate lane above 45 kDa is likely to represent SERPINE1 with an insertion. This has not been investigated further. Wild-type A549 and SERPINE1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with Anti-PAI1 antibody [EPR17272-21] ab182973 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-PAI1 antibody [EPR17272-21] (Anti-PAI1 antibody [EPR17272-21] ab182973) at 1/1000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: SERPINE1 knockout A549 cell lysate at 20 µg
Lane 3: HUVEC cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 48 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-epr17272-21-bsa-and-azide-free-ab226864--immunocytochemistry-immunofluorescence-img108093.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864)")
Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cells labeling PAI1 with Anti-PAI1 antibody [EPR17272-21] ab182973 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Hepa1-6 cells.
The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).
-ve control: PBS only, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAI1 antibody [EPR17272-21] ab182973).
![Flow Cytometry (Intracellular) - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-epr17272-21-bsa-and-azide-free-ab226864--flow-cytometry-intracellular-img140945.jpg "Flow Cytometry (Intracellular) - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864)")
Flow Cytometry (Intracellular) - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methonol-permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cells labeling PAI1 with Anti-PAI1 antibody [EPR17272-21] ab182973 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAI1 antibody [EPR17272-21] ab182973).
![Western blot - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-epr17272-21-bsa-and-azide-free-ab226864--western-blot-img54559.jpg "Western blot - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864)")
Western blot - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864)
Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
The expression level of mouse and rat PAI1 may be low in normal liver tissue (PMID: 21898503). This antibody detects no bands in rat liver.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAI1 antibody [EPR17272-21] ab182973).
All lanes: Western blot - Anti-PAI1 antibody [EPR17272-21] (Anti-PAI1 antibody [EPR17272-21] ab182973) at 1/1000 dilution
Lane 1: Rat placenta tissue lysate at 20 µg
Lane 2: Rat lung tissue lysate at 20 µg
Lane 3: Rat liver tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 20s
![Western blot - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-epr17272-21-bsa-and-azide-free-ab226864--western-blot-img30849.jpg "Western blot - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864)")
Western blot - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864)
Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
The expression level of mouse and rat PAI1 may be low in normal liver tissue (PMID: 21898503). This antibody detects a 37kDa extra band without BOI in mouse liver.
Exposure time:
Lane 1 : 3.25 seconds
Lane 2 : 180 secondsThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAI1 antibody [EPR17272-21] ab182973).
All lanes: Western blot - Anti-PAI1 antibody [EPR17272-21] (Anti-PAI1 antibody [EPR17272-21] ab182973) at 1/1000 dilution
Lane 1: Mouse placenta tissue lysate at 20 µg
Lane 2: Mouse liver tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
![Immunoprecipitation - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-epr17272-21-bsa-and-azide-free-ab226864--immunoprecipitation-img436068.jpg "Immunoprecipitation - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864)")
Immunoprecipitation - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAI1 antibody [EPR17272-21] ab182973).
Anti-PAI1 antibody [EPR17272-21] ab182973 at 1/30 immunoprecipitating PAI1 in SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate.
Lane 1 (input): Mouse placenta tissue lysate 10 μg
Lane 2 (+): Mouse placenta tissue lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-PAI1 antibody [EPR17272-21] ab182973 in mouse placenta tissue lysate
For western blotting, Anti-PAI1 antibody [EPR17272-21] ab182973 followed by VeriBlot for IP Detection Reagent (HRP) ab131366 VeriBlot for IP (HRP) were used for detection (1/5000).
Blocking/Diluting buffer and concentration: 5% NFDM/TBST.Lanes 1 - 3: Immunoprecipitation - Anti-PAI1 antibody [EPR17272-21] (Anti-PAI1 antibody [EPR17272-21] ab182973) at 1/30 dilution
Lanes 1 - 3: Immunoprecipitation - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864)
All lanes: Mouse placenta tissue lysate at 10 µg
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 8s
Anti-PAI1 antibody [EPR17272-21] ab182973 at 1/30 immunoprecipitating PAI1 in SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate.
Lane 1 (input): Mouse placenta tissue lysate 10 μg
Lane 2 (+): Mouse placenta tissue lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-PAI1 antibody [EPR17272-21] ab182973 in mouse placenta tissue lysate
For western blotting, Anti-PAI1 antibody [EPR17272-21] ab182973 followed by VeriBlot for IP Detection Reagent (HRP) ab131366 VeriBlot for IP (HRP) were used for detection (1/5000).
Blocking/Diluting buffer and concentration: 5% NFDM/TBST.![Immunoprecipitation - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-epr17272-21-bsa-and-azide-free-ab226864--immunoprecipitation-img436067.jpg "Immunoprecipitation - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864)")
Immunoprecipitation - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAI1 antibody [EPR17272-21] ab182973).
Anti-PAI1 antibody [EPR17272-21] ab182973 at 1/30 immunoprecipitating PAI1 in SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate.
Lane 1 (input): SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate (10µg)
Lane 2 (+): SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-PAI1 antibody [EPR17272-21] ab182973 in SW480 whole cell lysate
For western blotting, Anti-PAI1 antibody [EPR17272-21] ab182973 followed by VeriBlot for IP Detection Reagent (HRP) ab131366 VeriBlot for IP (HRP) was used for detection (1/5000).
Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
![Immunoprecipitation - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-epr17272-21-bsa-and-azide-free-ab226864--immunoprecipitation-img436068.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864)")
![Immunoprecipitation - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-epr17272-21-bsa-and-azide-free-ab226864--immunoprecipitation-img436067.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864)")
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