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Rabbit Recombinant Monoclonal PAI1 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples.

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Images

Western blot - Anti-PAI1 antibody [EPR17796] (AB187263), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR17796] (AB187263), expandable thumbnail
  • Western blot - Anti-PAI1 antibody [EPR17796] (AB187263), expandable thumbnail
  • Immunoprecipitation - Anti-PAI1 antibody [EPR17796] (AB187263), expandable thumbnail
  • Western blot - Anti-PAI1 antibody [EPR17796] (AB187263), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IF
Human
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/40

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Associated Products

Select an associated product type

11 products for Alternative Product

Target data

Function

Serine protease inhibitor. Inhibits TMPRSS7 (PubMed:15853774). Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots (PubMed:8481516, PubMed:9207454, PubMed:17912461). As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading (PubMed:9175705). Acts as a regulator of cell migration, independently of its role as protease inhibitor (PubMed:15001579, PubMed:9168821). It is required for stimulation of keratinocyte migration during cutaneous injury repair (PubMed:18386027). It is involved in cellular and replicative senescence (PubMed:16862142). Plays a role in alveolar type 2 cells senescence in the lung (By similarity). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (PubMed:25808697, PubMed:27046084).

Alternative names

Plasminogen activator inhibitor 1, PAI, PAI-1, Endothelial plasminogen activator inhibitor, Serpin E1, PLANH1, PAI1, SERPINE1

Recommended products

Rabbit Recombinant Monoclonal PAI1 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples.

Alternative names

Plasminogen activator inhibitor 1, PAI, PAI-1, Endothelial plasminogen activator inhibitor, Serpin E1, PLANH1, PAI1, SERPINE1

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR17796

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-PAI1 antibody [EPR17796] (ab187263), expandable thumbnail

    Western blot - Anti-PAI1 antibody [EPR17796] (ab187263)

    Lanes 1 - 4: Merged signal (red and green). Green - ab187263 observed at 48 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

    ab187263 was shown to react with PAI1 in wild-type A549 cells in Western blot. The band observed in the edited lysate lane above 45 kDa is likely to represent SERPINE1 with an insertion. This has not been investigated further. Wild-type A549 and SERPINE1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab187263 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-PAI1 antibody [EPR17796] (ab187263) at 1/1000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: SERPINE1 knockout A549 cell lysate at 20 µg

    Lane 3: HUVEC cell lysate at 20 µg

    Lane 4: HEK-293 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 45 kDa

    Observed band size: 48 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR17796] (ab187263), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR17796] (ab187263)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT1080 (Human fibrosarcoma cells) cells labeling PAI1 with ab187263 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/400 dilution (green). Cytoplasm staining on HT1080 cells was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab187263 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

  • Western blot - Anti-PAI1 antibody [EPR17796] (ab187263), expandable thumbnail

    Western blot - Anti-PAI1 antibody [EPR17796] (ab187263)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PAI1 antibody [EPR17796] (ab187263) at 1/1000 dilution

    Lane 1: Human fetal liver lysate at 10 µg

    Lane 2: Human fetal spleen lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 45 kDa

    Observed band size: 45 kDa

    Exposure time: 3min

  • Immunoprecipitation - Anti-PAI1 antibody [EPR17796] (ab187263), expandable thumbnail

    Immunoprecipitation - Anti-PAI1 antibody [EPR17796] (ab187263)

    PAI1 was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma) whole cell lysate with ab187263 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab187263 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1500 dilution.

    Lane 1: HepG2 whole cell lysate 10 μg (Input). Lane 2: ab187263 IP in HepG2 whole cell lysate. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab187263 in HepG2 whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 8 seconds.

    All lanes: Immunoprecipitation - Anti-PAI1 antibody [EPR17796] (ab187263)

    Predicted band size: 45 kDa, 62 kDa

  • Western blot - Anti-PAI1 antibody [EPR17796] (ab187263), expandable thumbnail

    Western blot - Anti-PAI1 antibody [EPR17796] (ab187263)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PAI1 antibody [EPR17796] (ab187263) at 1/10000 dilution

    All lanes: HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 45 kDa

    Observed band size: 45 kDa

    Exposure time: 3min

  • Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR17796] (ab187263), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR17796] (ab187263)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling PAI1 with ab187263 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/400 dilution (green). Cytoplasm staining on HepG2 cells was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab187263 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

  • Western blot - Anti-PAI1 antibody [EPR17796] (ab187263), expandable thumbnail

    Western blot - Anti-PAI1 antibody [EPR17796] (ab187263)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PAI1 antibody [EPR17796] (ab187263) at 1/5000 dilution

    All lanes: Human PAI1 full length protein at 0.01 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 45 kDa

    Observed band size: 70 kDa

    Exposure time: 30s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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