Rabbit Recombinant Monoclonal PAI1 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples.
Images
![Western blot - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (AB250925), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-epr17796-bsa-and-azide-free-ab250925--western-blot-img51751.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (AB250925)")
![Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (AB250925), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-epr17796-bsa-and-azide-free-ab250925--immunocytochemistry-immunofluorescence-img98486.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (AB250925)")
![Western blot - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (AB250925), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-epr17796-bsa-and-azide-free-ab250925--western-blot-img55028.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (AB250925)")
![Immunoprecipitation - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (AB250925), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-epr17796-bsa-and-azide-free-ab250925--immunoprecipitation-img670.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (AB250925)")
![Western blot - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (AB250925), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-epr17796-bsa-and-azide-free-ab250925--western-blot-img148445.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (AB250925)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Serine protease inhibitor. Inhibits TMPRSS7 (PubMed:15853774). Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots (PubMed:8481516, PubMed:9207454, PubMed:17912461). As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading (PubMed:9175705). Acts as a regulator of cell migration, independently of its role as protease inhibitor (PubMed:15001579, PubMed:9168821). It is required for stimulation of keratinocyte migration during cutaneous injury repair (PubMed:18386027). It is involved in cellular and replicative senescence (PubMed:16862142). Plays a role in alveolar type 2 cells senescence in the lung (By similarity). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (PubMed:25808697, PubMed:27046084).
Alternative names
Plasminogen activator inhibitor 1, PAI, PAI-1, Endothelial plasminogen activator inhibitor, Serpin E1, PLANH1, PAI1, SERPINE1
Recommended products
Rabbit Recombinant Monoclonal PAI1 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR17796
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab250925 is the carrier-free version of Anti-PAI1 antibody [EPR17796] ab187263.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (ab250925), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-epr17796-bsa-and-azide-free-ab250925--western-blot-img51751.jpg "Western blot - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (ab250925)")
Western blot - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (ab250925)
This data was developed using the same antibody clone in a different buffer formulation (Anti-PAI1 antibody [EPR17796] ab187263).
Lanes 1 - 4: Merged signal (red and green). Green - Anti-PAI1 antibody [EPR17796] ab187263 observed at 48 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
Anti-PAI1 antibody [EPR17796] ab187263 was shown to react with PAI1 in wild-type A549 cells in Western blot. The band observed in the edited lysate lane above 45 kDa is likely to represent SERPINE1 with an insertion. This has not been investigated further. Wild-type A549 and SERPINE1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with Anti-PAI1 antibody [EPR17796] ab187263 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-PAI1 antibody [EPR17796] (Anti-PAI1 antibody [EPR17796] ab187263) at 1/1000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: SERPINE1 knockout A549 cell lysate at 20 µg
Lane 3: HUVEC cell lysate at 20 µg
Lane 4: HEK-293 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 48 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (ab250925), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-epr17796-bsa-and-azide-free-ab250925--immunocytochemistry-immunofluorescence-img98486.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (ab250925)")
Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (ab250925)
This data was developed using Anti-PAI1 antibody [EPR17796] ab187263, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT1080 (Human fibrosarcoma cells) cells labeling PAI1 with Anti-PAI1 antibody [EPR17796] ab187263 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/400 dilution (green). Cytoplasm staining on HT1080 cells was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: Anti-PAI1 antibody [EPR17796] ab187263 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.![Western blot - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (ab250925), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-epr17796-bsa-and-azide-free-ab250925--western-blot-img55028.jpg "Western blot - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (ab250925)")
Western blot - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (ab250925)
This data was developed using Anti-PAI1 antibody [EPR17796] ab187263, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-PAI1 antibody [EPR17796] (Anti-PAI1 antibody [EPR17796] ab187263) at 1/1000 dilution
Lane 1: Human fetal liver lysate at 10 µg
Lane 2: Human fetal spleen lysate at 10 µg
SecondaryAll lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 3min
![Immunoprecipitation - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (ab250925), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-epr17796-bsa-and-azide-free-ab250925--immunoprecipitation-img670.jpg "Immunoprecipitation - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (ab250925)")
Immunoprecipitation - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (ab250925)
This data was developed using Anti-PAI1 antibody [EPR17796] ab187263, the same antibody clone in a different buffer formulation.PAI1 was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma) whole cell lysate with Anti-PAI1 antibody [EPR17796] ab187263 at 1/40 dilution. Western blot was performed from the immunoprecipitate using Anti-PAI1 antibody [EPR17796] ab187263 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1500 dilution.
Lane 1: HepG2 whole cell lysate 10 µg (Input). Lane 2: Anti-PAI1 antibody [EPR17796] ab187263 IP in HepG2 whole cell lysate. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-PAI1 antibody [EPR17796] ab187263 in HepG2 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 8 seconds.
All lanes: Immunoprecipitation - Anti-PAI1 antibody [EPR17796] (Anti-PAI1 antibody [EPR17796] ab187263)
Predicted band size: 45 kDa, 62 kDa
![Western blot - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (ab250925), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-epr17796-bsa-and-azide-free-ab250925--western-blot-img148445.jpg "Western blot - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (ab250925)")
Western blot - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (ab250925)
This data was developed using Anti-PAI1 antibody [EPR17796] ab187263, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-PAI1 antibody [EPR17796] (Anti-PAI1 antibody [EPR17796] ab187263) at 1/10000 dilution
All lanes: HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 3min
![Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (ab250925), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-epr17796-bsa-and-azide-free-ab250925--immunocytochemistry-immunofluorescence-img14995.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (ab250925)")
Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (ab250925)
This data was developed using Anti-PAI1 antibody [EPR17796] ab187263, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling PAI1 with Anti-PAI1 antibody [EPR17796] ab187263 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/400 dilution (green). Cytoplasm staining on HepG2 cells was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: Anti-PAI1 antibody [EPR17796] ab187263 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.![Western blot - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (ab250925), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-epr17796-bsa-and-azide-free-ab250925--western-blot-img86270.jpg "Western blot - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (ab250925)")
Western blot - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (ab250925)
This data was developed using Anti-PAI1 antibody [EPR17796] ab187263, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-PAI1 antibody [EPR17796] (Anti-PAI1 antibody [EPR17796] ab187263) at 1/5000 dilution
All lanes: Human PAI1 full length protein at 0.01 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 45 kDa
Observed band size: 70 kDa
Exposure time: 30s
![Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (ab250925), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-epr17796-bsa-and-azide-free-ab250925--immunocytochemistry-immunofluorescence-img14995.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (ab250925)")
![Western blot - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (ab250925), expandable thumbnail](https://content.abcam.com/products/images/pai1-antibody-epr17796-bsa-and-azide-free-ab250925--western-blot-img86270.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PAI1 antibody [EPR17796] - BSA and Azide free (ab250925)")
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