Anti-PAICS/PAIS antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAICS/PAIS antibody (AB240743)

Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for PAICS/PAIS using ab240743 at 1 μg/ml in immunohistochemical analysis. DAB staining.

• IP

Supplier Data

Immunoprecipitation - Anti-PAICS/PAIS antibody (AB240743)

PAICS/PAIS was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab240743 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab240743 at 0.4 μg/ml.

Lane 1 : ab240743 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-PAICS/PAIS antibody (ab240743)

Predicted band size: 47 kDa

false

• WB

Supplier Data

Western blot - Anti-PAICS/PAIS antibody (AB240743)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-PAICS/PAIS antibody (ab240743) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 47 kDa

false

Exposure time: 30s