AB181359

Anti-PAIP1 antibody [EPR13259]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal PAIP1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Polyadenylate-binding protein-interacting protein 1, PABP-interacting protein 1, PAIP-1, Poly(A)-binding protein-interacting protein 1, PAIP1

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-PAIP1 antibody [EPR13259] (AB181359)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PAIP1 antibody [EPR13259] (AB181359)

Immunofluorescence analysis of HeLa cells labeling PAIP1 with ab181359 at 1/50 dilution (green). DAPI nuclear staining (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAIP1 antibody [EPR13259] (AB181359)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAIP1 antibody [EPR13259] (AB181359)

Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling PAIP1 with ab181359 at 1/50 dilution.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-PAIP1 antibody [EPR13259] (AB181359)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PAIP1 antibody [EPR13259] (AB181359)

Intracellular flow cytometric analysis of permeabilized 293 cells using ab181359 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAIP1 antibody [EPR13259] (AB181359)

Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling PAIP1 with ab181359 at 1/50 dilution.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-PAIP1 antibody [EPR13259] (AB181359)

Western blot analysis on immunoprecipitation pellet from (1) HeLa cell lysate or (2) 1XPBS (negative control) using ab181359 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG

All lanes:

Immunoprecipitation - Anti-PAIP1 antibody [EPR13259] (ab181359)

Predicted band size: 54 kDa

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Supplier Data

Western blot - Anti-PAIP1 antibody [EPR13259] (AB181359)

All lanes:

Western blot - Anti-PAIP1 antibody [EPR13259] (ab181359) at 1/1000 dilution

Lane 1:

HepG2 cell lysates at 10 µg

Lane 2:

HeLa cell lysates at 10 µg

Lane 3:

293T cell lysates at 10 µg

Predicted band size: 54 kDa

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Carrier free

Anti-PAIP1 antibody [EPR13259] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13259

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB, IP, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs. Its stimulatory activity on translation is mediated via its action on PABPC1. Competes with PAIP2 for binding to PABPC1. Its association with EIF4A and PABPC1 may potentiate contacts between mRNA termini. May also be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.. (Microbial infection) Upon interaction with SARS coronavirus SARS-CoV NSP3 protein, plays an important role in viral protein synthesis.

See full target information Polyadenylate-binding protein-interacting protein 1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Reproductive sciences (Thousand Oaks, Calif.) 29:2647-2658 PubMed35378711

2022

Circ_0005576 Exerts an Oncogenic Role in Cervical Cancer via miR-1305-Dependent Regulation of PAIP1.

Applications

Unspecified application

Species

Unspecified reactive species

Yajing Wang,Fang Du,Zongyuan Xie,Junhao Lai,Yuanjie Li,Yongping Xu,Rui Tong

Head & neck 42:3623-3637 PubMed32827170

2020

Effects of inducing apoptosis and inhibiting proliferation of siRNA on polyadenylate-binding protein-interacting protein 1 in tongue cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Huixu Xie,Lisa Yang,Qin Hu,Yingqi Song,Xiaoyi Wang,Liming Zhou,Longjiang Li

Aging 11:1045-1061 PubMed30745468

2019

Measuring biological age in mice using differential mass spectrometry.

Applications

Unspecified application

Species

Unspecified reactive species

Harris Bell-Temin,Matthew J Yousefzadeh,Andrey Bondarenko,Ellen Quarles,Jacqueline Jones-Laughner,Paul D Robbins,Warren Ladiges,Laura J Niedernhofer,Nathan A Yates

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PAIP1 antibody [EPR13259]

Immunocytochemistry/ Immunofluorescence - Anti-PAIP1 antibody [EPR13259] (AB181359)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAIP1 antibody [EPR13259] (AB181359)

Flow Cytometry (Intracellular) - Anti-PAIP1 antibody [EPR13259] (AB181359)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAIP1 antibody [EPR13259] (AB181359)

Immunoprecipitation - Anti-PAIP1 antibody [EPR13259] (AB181359)

Western blot - Anti-PAIP1 antibody [EPR13259] (AB181359)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

Product promise