AB302506

Anti-PAK1 antibody [EPR26067-45]

BOND RX™ Validated
20ul selling size
RabMAb
Recombinant
KO Validated
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Rabbit Recombinant Monoclonal PAK1 antibody. Suitable for Dot, IP, IHC-P, WB and reacts with Synthetic peptide - Human, Human, Mouse, Rat samples.

View Alternative Names

Serine/threonine-protein kinase PAK 1, Alpha-PAK, p21-activated kinase 1, p65-PAK, PAK-1, PAK1

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK1 antibody [EPR26067-45] (AB302506)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK1 antibody [EPR26067-45] (AB302506)

Immunohistochemical analysis of paraffin-embedded A Wild-type HAP1 (h tissue labeling PAK1 with ab302506 at 1/500 (1.146 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Cytoplasmic staining on (A) wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) cell pellet and no staining on (B) PAK1 knockout HAP1 cell pellet. The section was incubated with ab302506 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK1 antibody [EPR26067-45] (AB302506)

Immunohistochemical analysis of paraffin-embedded Human gastic cancer tissue labeling PAK1 with ab302506 at 1/5000 (0.115 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Cytoplasmic staining on human gastric cancer tissue and no staining on adjacent tissue. The section was incubated with ab302506 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK1 antibody [EPR26067-45] (AB302506)

Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling PAK1 with ab302506 at 1/5000 (0.115 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Cytoplasmic staining on human ovarian cancer. The section was incubated with ab302506 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK1 antibody [EPR26067-45] (AB302506)

Immunohistochemical analysis of paraffin-embedded Mouse pancreas cance tissue labeling PAK1 with ab302506 at 1/5000 (0.115 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Cytoplasmic staining on mouse pancreas cancer. The section was incubated with ab302506 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK1 antibody [EPR26067-45] (AB302506)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling PAK1 with ab302506 at 1/2000 (0.287 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Cytoplasmic staining on rat cerebrum. The section was incubated with ab302506 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK1 antibody [EPR26067-45] (AB302506)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling PAK1 with ab302506 at 1/2000 (0.287 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Cytoplasmic staining on mouse cerebrum. The section was incubated with ab302506 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Supplier Data

Western blot - Anti-PAK1 antibody [EPR26067-45] (AB302506)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-PAK1 antibody [EPR26067-45] (ab302506) at 1/1000 dilution

Lane 1:

HEK-293 (human embryonic kidney epithelial cell), whole cell lysate 20 µg

Lane 2:

SK-OV-3 (human ovarian cancer epithelial cell), whole cell lysate 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate 20 µg

Lane 4:

C6 (rat glial tumor glial cell), whole cell lysate 20 µg

Lane 5:

Mouse brain tissue lysate 20 µg

Lane 6:

Rat brain tissue lysate 20 µg

Lane 7:

Human kidney tissue lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 68 kDa

false

Supplier Data

Dot Blot - Anti-PAK1 antibody [EPR26067-45] (AB302506)

Dot blot analysis of PAK1 using ab302506 at 1 : 1000 (0.573 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution. Exposure time : 3 minutes Blocking and diluting buffer and concentration : 5% NFDM/TBST

Supplier Data

Western blot - Anti-PAK1 antibody [EPR26067-45] (AB302506)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

In Western blot, ab302506 was shown to bind specifically to PAK1. A band was observed at 68 kDa in wild-type HAP1 cell lysates whereas no signal was observed at this size in PAK1 knockout cell lysates.

All lanes:

Western blot - Anti-PAK1 antibody [EPR26067-45] (ab302506) at 1/1000 dilution

Lane 1:

Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate at 20 µg

Lane 2:

PAK1 knockout HAP1 whole cell lysate at 20 µg

Lane 3:

SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 68 kDa

false

Exposure time: 37s

Carrier free

Anti-PAK1 antibody [EPR26067-45] (BSA and Azide free)
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-PAK1 antibody [EPR26067-45]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-PAK1 antibody [EPR26067-45]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-PAK1 antibody [EPR26067-45]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26067-45

Isotype

IgG

Carrier free

Reacts with

Rat, Mouse, Human

Applications

IP, IHC-P, Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Synthetic peptide - Human": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, proliferation, apoptosis, mitosis, and in vesicle-mediated transport processes (PubMed : 10551809, PubMed : 11896197, PubMed : 12876277, PubMed : 14585966, PubMed : 15611088, PubMed : 17726028, PubMed : 17989089, PubMed : 30290153, PubMed : 17420447). Can directly phosphorylate BAD and protects cells against apoptosis (By similarity). Activated by interaction with CDC42 and RAC1 (PubMed : 8805275, PubMed : 9528787). Functions as a GTPase effector that links the Rho-related GTPases CDC42 and RAC1 to the JNK MAP kinase pathway (PubMed : 8805275, PubMed : 9528787). Phosphorylates and activates MAP2K1, and thereby mediates activation of downstream MAP kinases (By similarity). Involved in the reorganization of the actin cytoskeleton, actin stress fibers and of focal adhesion complexes (PubMed : 9032240, PubMed : 9395435). Phosphorylates the tubulin chaperone TBCB and thereby plays a role in the regulation of microtubule biogenesis and organization of the tubulin cytoskeleton (PubMed : 15831477). Plays a role in the regulation of insulin secretion in response to elevated glucose levels (PubMed : 22669945). Part of a ternary complex that contains PAK1, DVL1 and MUSK that is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ) (By similarity). Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2 (PubMed : 12624090). Phosphorylates MYL9/MLC2 (By similarity). Phosphorylates RAF1 at 'Ser-338' and 'Ser-339' resulting in : activation of RAF1, stimulation of RAF1 translocation to mitochondria, phosphorylation of BAD by RAF1, and RAF1 binding to BCL2 (PubMed : 11733498). Phosphorylates SNAI1 at 'Ser-246' promoting its transcriptional repressor activity by increasing its accumulation in the nucleus (PubMed : 15833848). In podocytes, promotes NR3C2 nuclear localization (By similarity). Required for atypical chemokine receptor ACKR2-induced phosphorylation of LIMK1 and cofilin (CFL1) and for the up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (PubMed : 23633677). In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3, maybe through CFL1 phosphorylation and inactivation (By similarity). Plays a role in RUFY3-mediated facilitating gastric cancer cells migration and invasion (PubMed : 25766321). In response to DNA damage, phosphorylates MORC2 which activates its ATPase activity and facilitates chromatin remodeling (PubMed : 23260667). In neurons, plays a crucial role in regulating GABA(A) receptor synaptic stability and hence GABAergic inhibitory synaptic transmission through its role in F-actin stabilization (By similarity). In hippocampal neurons, necessary for the formation of dendritic spines and excitatory synapses; this function is dependent on kinase activity and may be exerted by the regulation of actomyosin contractility through the phosphorylation of myosin II regulatory light chain (MLC) (By similarity). Along with GIT1, positively regulates microtubule nucleation during interphase (PubMed : 27012601). Phosphorylates FXR1, promoting its localization to stress granules and activity (PubMed : 20417602). Phosphorylates ILK on 'Thr-173' and 'Ser-246', promoting nuclear export of ILK (PubMed : 17420447).

See full target information PAK1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PAK1 antibody [EPR26067-45]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK1 antibody [EPR26067-45] (AB302506)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK1 antibody [EPR26067-45] (AB302506)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK1 antibody [EPR26067-45] (AB302506)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK1 antibody [EPR26067-45] (AB302506)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK1 antibody [EPR26067-45] (AB302506)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK1 antibody [EPR26067-45] (AB302506)

Western blot - Anti-PAK1 antibody [EPR26067-45] (AB302506)

Dot Blot - Anti-PAK1 antibody [EPR26067-45] (AB302506)

Western blot - Anti-PAK1 antibody [EPR26067-45] (AB302506)

Related conjugates and formulations (4)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Product promise