Rabbit Recombinant Monoclonal PAK3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications.
Images
![Western blot - Anti-PAK3 antibody [EP797Y] (AB40808), expandable thumbnail](https://content.abcam.com/products/images/pak3-antibody-ep797y-ab40808--western-blot-img158953.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PAK3 antibody [EP797Y] (AB40808)")
Publications
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- Journal of Cancer 13:153-1612022PAK3 promotes the metastasis of hepatocellular carcinoma by regulating EMT process.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhi Gao,Mengya Zhong,Zhijian Ye,Zhengxin Wu,Yubo Xiong,Jinsong Ma,Huiyu Chen,Yuekun Zhu,Yan Yang,Yongxiang Zhao,Zhiyong ZhangPubMed 34976179
- Cell biology international 44:2284-22922020LncRNA TTN-AS1/miR-134-5p/PAK3 axis regulates the radiosensitivity of human large intestine cancer cells through the P21 pathway and AKT/GSK-3β/β-catenin pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenkui Zuo,Shuling Ji,Lulu He,Yage Zhang,Zining Peng,Jiarui HanPubMed 32749739
- Brain pathology (Zurich, Switzerland) 30:319-3312019Synaptic actin stabilization protein loss in Down syndrome and Alzheimer disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulie C Lauterborn,Conor D Cox,See Wing Chan,Peter W Vanderklish,Gary Lynch,Christine M GallPubMed 31410926
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | Flow Cyt | WB | |
|---|---|---|---|
| Human | Tested | Not recommended | Tested |
| Mouse | Predicted | Not recommended | Predicted |
| Rat | Predicted | Not recommended | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as a downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development. In hippocampal neurons, necessary for the formation of dendritic spines and excitatory synapses; this function is dependent on kinase activity and may be exerted by the regulation of actomyosin contractility through the phosphorylation of myosin II regulatory light chain (MLC) (By similarity).
Alternative names
OPHN3, PAK3, Serine/threonine-protein kinase PAK 3, Beta-PAK, Oligophrenin-3, p21-activated kinase 3, PAK-3
Recommended products
Rabbit Recombinant Monoclonal PAK3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP797Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-PAK3 antibody [EP797Y] (ab40808), expandable thumbnail](https://content.abcam.com/products/images/pak3-antibody-ep797y-ab40808--western-blot-img158953.jpg "Western blot - Anti-PAK3 antibody [EP797Y] (ab40808)")
Western blot - Anti-PAK3 antibody [EP797Y] (ab40808)
All lanes: Western blot - Anti-PAK3 antibody [EP797Y] (ab40808) at 1/50000 dilution
All lanes: fetal brain lysate at 10 µg
Predicted band size: 62 kDa
Observed band size: 65 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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