Anti-PAK3 antibody [EP797Y] - BSA and Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK3 antibody [EP797Y] - BSA and Azide free (AB247294)

This data was developed using ab40808, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human brain tissue labeling PAK3 with ab40808 at 1/50 dilution (3.98 μg/ml).

Positive staining on human brain.

The section was incubated with ab40808 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK3 antibody [EP797Y] - BSA and Azide free (AB247294)

This data was developed using ab40808, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue labeling PAK3 with ab40808 at 1/50 dilution (3.98 μg/ml).

Positive staining on mouse brain.

The section was incubated with ab40808 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK3 antibody [EP797Y] - BSA and Azide free (AB247294)

This data was developed using ab40808, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling PAK3 with ab40808 at 1/50 dilution (3.98 μg/ml).

Positive staining on rat brain.

The section was incubated with ab40808 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 mins.

• WB

Lab

Western blot - Anti-PAK3 antibody [EP797Y] - BSA and Azide free (AB247294)

This data was developed using ab40808, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000,000 dilution.

Negative samples : liver and kidney (PMID : 9731525).

All lanes:

Western blot - Anti-PAK3 antibody [EP797Y] (<a href='/en-us/products/primary-antibodies/pak3-antibody-ep797y-ab40808'>ab40808</a>) at 1/2000 dilution

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Lane 3:

Human kidney tissue lysate at 20 µg

Lane 4:

Mouse brain tissue lysate at 20 µg

Lane 5:

Mouse liver tissue lysate at 20 µg

Lane 6:

Mouse kidney tissue lysate at 20 µg

Lane 7:

Rat brain tissue lysate at 20 µg

Lane 8:

Rat kidney tissue lysate at 20 µg

Lane 9:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 65 kDa,36 kDa

false

Exposure time: 180s

• WB

Unknown

Western blot - Anti-PAK3 antibody [EP797Y] - BSA and Azide free (AB247294)

This data was developed using ab40808, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PAK3 antibody [EP797Y] - BSA and Azide free (ab247294) at 1/50000 dilution

All lanes:

fetal brain lysate at 10 µg

Predicted band size: 62 kDa

Observed band size: 65 kDa

false