Rabbit Recombinant Monoclonal PAK4 antibody. Suitable for WB and reacts with Rat, Mouse, Recombinant full length protein - Human, Human samples.
Images
![Western blot - Anti-PAK4 antibody [EPR25195-73] (AB300503), expandable thumbnail](https://content.abcam.com/products/images/pak4-antibody-epr25195-73-ab300503--western-blot-img431272.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PAK4 antibody [EPR25195-73] (AB300503)")
![Western blot - Anti-PAK4 antibody [EPR25195-73] (AB300503), expandable thumbnail](https://content.abcam.com/products/images/pak4-antibody-epr25195-73-ab300503--western-blot-img400463.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PAK4 antibody [EPR25195-73] (AB300503)")
![Western blot - Anti-PAK4 antibody [EPR25195-73] (AB300503), expandable thumbnail](https://content.abcam.com/products/images/pak4-antibody-epr25195-73-ab300503--western-blot-img400462.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PAK4 antibody [EPR25195-73] (AB300503)")
![Western blot - Anti-PAK4 antibody [EPR25195-73] (AB300503), expandable thumbnail](https://content.abcam.com/products/images/pak4-antibody-epr25195-73-ab300503--western-blot-img400461.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PAK4 antibody [EPR25195-73] (AB300503)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt (Intra) | ICC/IF | IHC-P | WB | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
| Recombinant full length protein - Human | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant full length protein - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant full length protein - Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant full length protein - Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human, Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Recombinant full length protein - Human | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Target data
Function
Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN.
Alternative names
KIAA1142, PAK4, Serine/threonine-protein kinase PAK 4, p21-activated kinase 4, PAK-4
Recommended products
Rabbit Recombinant Monoclonal PAK4 antibody. Suitable for WB and reacts with Rat, Mouse, Recombinant full length protein - Human, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR25195-73
- Purification technique
- Affinity purification Protein A
- Specificity
We recommend using freshly made lysate to perform western blot testing.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-PAK4 antibody [EPR25195-73] (ab300503), expandable thumbnail](https://content.abcam.com/products/images/pak4-antibody-epr25195-73-ab300503--western-blot-img431272.jpg "Western blot - Anti-PAK4 antibody [EPR25195-73] (ab300503)")
Western blot - Anti-PAK4 antibody [EPR25195-73] (ab300503)
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
We recommend using freshly made lysate to perform western blot testing.
All lanes: Western blot - Anti-PAK4 antibody [EPR25195-73] (ab300503) at 1/1000 dilution
Lane 1: 293T (Human embryonic kidney epithelial cell) whole cell lysate, frozen lysate at 20 µg
Lane 2: 293T (Human embryonic kidney epithelial cell) whole cell lysate, fresh lysate
Lane 3: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate, frozen lysate at 20 µg
Lane 4: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate, fresh lysate
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 68 kDa
Exposure time: 40s
![Western blot - Anti-PAK4 antibody [EPR25195-73] (ab300503), expandable thumbnail](https://content.abcam.com/products/images/pak4-antibody-epr25195-73-ab300503--western-blot-img400463.jpg "Western blot - Anti-PAK4 antibody [EPR25195-73] (ab300503)")
Western blot - Anti-PAK4 antibody [EPR25195-73] (ab300503)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-PAK4 antibody [EPR25195-73] (ab300503) at 1/1000 dilution
Lane 1: Purified His-tagged human PAK4 recombinant protein
Lane 2: Purified His-tagged human PAK5 recombinant protein
Lane 3: Purified His-tagged human PAK6 recombinant protein
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Performed under reducing conditions.
Observed band size: 68 kDa
Exposure time: 3min
![Western blot - Anti-PAK4 antibody [EPR25195-73] (ab300503), expandable thumbnail](https://content.abcam.com/products/images/pak4-antibody-epr25195-73-ab300503--western-blot-img400462.jpg "Western blot - Anti-PAK4 antibody [EPR25195-73] (ab300503)")
Western blot - Anti-PAK4 antibody [EPR25195-73] (ab300503)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-PAK4 antibody [EPR25195-73] (ab300503) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 3: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
Lane 4: THP-1 (human monocytic leukemia monocyte) whole cell lysate
Lane 5: Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
Lane 6: NR8383 (rattus norvegicus lu macrophage (alveolar)) whole cell lysate 20
Lane 7: C2C12 (mouse myoblasts myoblast), whole cell lysate
Lane 8: LNCaP (human prostate carcinoma epithelial cell), whole cell lysate
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Performed under reducing conditions.
Observed band size: 68 kDa
![Western blot - Anti-PAK4 antibody [EPR25195-73] (ab300503), expandable thumbnail](https://content.abcam.com/products/images/pak4-antibody-epr25195-73-ab300503--western-blot-img400461.jpg "Western blot - Anti-PAK4 antibody [EPR25195-73] (ab300503)")
Western blot - Anti-PAK4 antibody [EPR25195-73] (ab300503)
Blocking and diluting buffer and concentration: ½ volume of Odyssey Blocking Buffer (TBS)+ ½ volume of 0.1% TBS
All lanes: Western blot - Anti-PAK4 antibody [EPR25195-73] (ab300503) at 1/1000 dilution
All lanes: RAW264.7 treated with /ml lipopolysaccharide (LPS) for 7 hours, whole cell lysate 40 µg
SecondaryLanes 1 - 3: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/20000 dilution
Lanes 1 - 3: Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 68 kDa
Downloads
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- Visit the general protocols
- Visit troubleshooting
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