Knockout Tested Rabbit Recombinant Monoclonal PAK4 antibody. Carrier free. Suitable for IHC-P, WB, IP, Flow Cyt (Intra) and reacts with Human, Recombinant fragment - Human samples.
Images
![Western blot - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (AB314766), expandable thumbnail](https://content.abcam.com/products/images/pak4-antibody-epr28630-82-bsa-and-azide-free-ab314766--western-blot-img422467.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (AB314766)")
![Western blot - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (AB314766), expandable thumbnail](https://content.abcam.com/products/images/pak4-antibody-epr28630-82-bsa-and-azide-free-ab314766--western-blot-img422466.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (AB314766)")
![Western blot - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (AB314766), expandable thumbnail](https://content.abcam.com/products/images/pak4-antibody-epr28630-82-bsa-and-azide-free-ab314766--western-blot-img422465.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (AB314766)")
![Immunoprecipitation - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (AB314766), expandable thumbnail](https://content.abcam.com/products/images/pak4-antibody-epr28630-82-bsa-and-azide-free-ab314766--immunoprecipitation-img422489.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (AB314766)")
![Flow Cytometry (Intracellular) - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (AB314766), expandable thumbnail](https://content.abcam.com/products/images/pak4-antibody-epr28630-82-bsa-and-azide-free-ab314766--flow-cytometry-intracellular-img422505.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (AB314766)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | IP | Flow Cyt (Intra) | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant fragment - Human | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Recombinant fragment - Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN.
Alternative names
KIAA1142, PAK4, Serine/threonine-protein kinase PAK 4, p21-activated kinase 4, PAK-4
Recommended products
Knockout Tested Rabbit Recombinant Monoclonal PAK4 antibody. Carrier free. Suitable for IHC-P, WB, IP, Flow Cyt (Intra) and reacts with Human, Recombinant fragment - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR28630-82
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody does not cross-react with human PAK5 and PAK6.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab314766 is the carrier-free version of Anti-PAK4 antibody [EPR28630-82] ab314765.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
![Western blot - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766), expandable thumbnail](https://content.abcam.com/products/images/pak4-antibody-epr28630-82-bsa-and-azide-free-ab314766--western-blot-img422467.jpg "Western blot - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766)")
Western blot - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766)
This data was developed using Anti-PAK4 antibody [EPR28630-82] ab314765, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This target was tested in fresh and frozen LNCaP and Hela, and it was concluded that PAK4 is the easy target for degradation.Lane 3-5 was freshly made and used for Western blotting immediately to minimize protein degradation.
Exposure time: Lane 1, Lane 4-5: 70 seconds; Lane 2: 180 seconds; Lane 3: 26 secondsAll lanes: Western blot - Anti-PAK4 antibody [EPR28630-82] (Anti-PAK4 antibody [EPR28630-82] ab314765) at 1/1000 dilution
Lanes 1 and 3: LNCaP (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg
Lanes 2 and 4: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 5: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 52 kDa, 68 kDa
![Western blot - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766), expandable thumbnail](https://content.abcam.com/products/images/pak4-antibody-epr28630-82-bsa-and-azide-free-ab314766--western-blot-img422466.jpg "Western blot - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766)")
Western blot - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766)
This data was developed using Anti-PAK4 antibody [EPR28630-82] ab314765, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-PAK4 antibody [EPR28630-82] ab314765 was shown to bind specifically to PAK4. Target of interest was observed at 68 kDa and 52 KD in wild-type HEK293T cell lysates (lane 1, wild-type cell line ab255449) with no signal observed at this size in PAK4 knockout cell line (lane 2, knockout cell line Human PAK4 knockout HEK-293T cell line ab266807/ knockout cell lysate Human PAK4 knockout HEK-293T cell lysate ab258560). The band (50 kDa) beneath the target band (68 kDa) is likely to be degraded target fragments. In western blot, anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.All lanes: Western blot - Anti-PAK4 antibody [EPR28630-82] (Anti-PAK4 antibody [EPR28630-82] ab314765) at 1/1000 dilution
Lane 1: Western blot - Human wild-type HEK-293T cell line (ab255449) at 20 µg
Lane 2: Western blot - Human PAK4 knockout HEK-293T cell lysate (Human PAK4 knockout HEK-293T cell lysate ab258560) at 20 µg
Lane 3: PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: Human testis lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 52 kDa, 68 kDa
Exposure time: 70s
![Western blot - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766), expandable thumbnail](https://content.abcam.com/products/images/pak4-antibody-epr28630-82-bsa-and-azide-free-ab314766--western-blot-img422465.jpg "Western blot - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766)")
Western blot - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766)
This data was developed using Anti-PAK4 antibody [EPR28630-82] ab314765, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody does not cross-react with human PAK5 and human PAK6. All the recombinant proteins were made in-house. In western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.All lanes: Western blot - Anti-PAK4 antibody [EPR28630-82] (Anti-PAK4 antibody [EPR28630-82] ab314765) at 1/1000 dilution
Lane 1: Purified His-tagged human PAK4 recombinant protein at 20 ng
Lane 2: Purified His-tagged human PAK5 recombinant protein at 100 ng
Lane 3: Purified His-tagged human PAK6 recombinant protein at 20 ng
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 80 kDa
Exposure time: 48s
![Immunoprecipitation - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766), expandable thumbnail](https://content.abcam.com/products/images/pak4-antibody-epr28630-82-bsa-and-azide-free-ab314766--immunoprecipitation-img422489.jpg "Immunoprecipitation - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766)")
Immunoprecipitation - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766)
This data was developed using Anti-PAK4 antibody [EPR28630-82] ab314765, the same antibody clone in a different buffer formulation.
PAK4 was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) whole cell lysate with Anti-PAK4 antibody [EPR28630-82] ab314765 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-PAK4 antibody [EPR28630-82] ab314765 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: 293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 2: Anti-PAK4 antibody [EPR28630-82] ab314765 IP in 293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-PAK4 antibody [EPR28630-82] ab314765 in 293T whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.All lanes: Immunoprecipitation - Anti-PAK4 antibody [EPR28630-82] (Anti-PAK4 antibody [EPR28630-82] ab314765) at 1/30 dilution
All lanes: 293T (human embryonic kidney epithelial cell) whole cell lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 15s
![Flow Cytometry (Intracellular) - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766), expandable thumbnail](https://content.abcam.com/products/images/pak4-antibody-epr28630-82-bsa-and-azide-free-ab314766--flow-cytometry-intracellular-img422505.jpg "Flow Cytometry (Intracellular) - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766)")
Flow Cytometry (Intracellular) - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766)
This data was developed using Anti-PAK4 antibody [EPR28630-82] ab314765, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized PAK4 KO HEK293T (human PAK4 knockout embryonic kidney epithelial cell, Left) / Parental HEK293T (Right) cells labelling PAK4 with Anti-PAK4 antibody [EPR28630-82] ab314765 at 1/50 dilution (1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766), expandable thumbnail](https://content.abcam.com/products/images/pak4-antibody-epr28630-82-bsa-and-azide-free-ab314766--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img422226.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766)
This data was developed using Anti-PAK4 antibody [EPR28630-82] ab314765, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) Parental HEK-293T (human embryonic kidney epithelial cell) cell pellet; (B) PAK4 knock-out HEK-293T cell pellet. Tissue labeling PAK4 with Anti-PAK4 antibody [EPR28630-82] ab314765 at 1/500 (1.052 ug/ml) dilution, followed by a ready to use rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on (A) parental HEK-293T cell pellet, no staining on (B) PAK4 knock-out HEK-293T cell pellet. The section was incubated with Anti-PAK4 antibody [EPR28630-82] ab314765 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766), expandable thumbnail](https://content.abcam.com/products/images/pak4-antibody-epr28630-82-bsa-and-azide-free-ab314766--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img422225.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766)
This data was developed using Anti-PAK4 antibody [EPR28630-82] ab314765, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling PAK4 with Anti-PAK4 antibody [EPR28630-82] ab314765 at 1/50 (10.52 ug/ml) dilution, followed by a ready to use rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on human colon carcinoma (PMID: 28542136). The section was incubated with Anti-PAK4 antibody [EPR28630-82] ab314765 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766), expandable thumbnail](https://content.abcam.com/products/images/pak4-antibody-epr28630-82-bsa-and-azide-free-ab314766--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img422224.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766)
This data was developed using Anti-PAK4 antibody [EPR28630-82] ab314765, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling PAK4 with Anti-PAK4 antibody [EPR28630-82] ab314765 at 1/50 (10.52 ug/ml) dilution, followed by a ready to use rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on human breast carcinoma (PMID: 28407679). The section was incubated with Anti-PAK4 antibody [EPR28630-82] ab314765 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766), expandable thumbnail](https://content.abcam.com/products/images/pak4-antibody-epr28630-82-bsa-and-azide-free-ab314766--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img422223.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766)
This data was developed using Anti-PAK4 antibody [EPR28630-82] ab314765, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissue labeling PAK4 with Anti-PAK4 antibody [EPR28630-82] ab314765 at 1/50 (10.52 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on human endometrial carcinoma. The section was incubated with Anti-PAK4 antibody [EPR28630-82] ab314765 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766), expandable thumbnail](https://content.abcam.com/products/images/pak4-antibody-epr28630-82-bsa-and-azide-free-ab314766--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img422226.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766), expandable thumbnail](https://content.abcam.com/products/images/pak4-antibody-epr28630-82-bsa-and-azide-free-ab314766--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img422225.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766), expandable thumbnail](https://content.abcam.com/products/images/pak4-antibody-epr28630-82-bsa-and-azide-free-ab314766--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img422224.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766), expandable thumbnail](https://content.abcam.com/products/images/pak4-antibody-epr28630-82-bsa-and-azide-free-ab314766--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img422223.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK4 antibody [EPR28630-82] - BSA and Azide free (ab314766)")
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