Skip to main content

Rabbit Recombinant Monoclonal PAK4 phospho S474 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

Be the first to review this product! Submit a review

Images

Flow Cytometry (Intracellular) - Anti-PAK4 + PAK5 + PAK6 (phospho S474 + S560 + S602) antibody [EPR2471(2)] (AB134097), expandable thumbnail
  • Western blot - Anti-PAK4 + PAK5 + PAK6 (phospho S474 + S560 + S602) antibody [EPR2471(2)] (AB134097), expandable thumbnail
  • OI-RD Scanning - Anti-PAK4 + PAK5 + PAK6 (phospho S474 + S560 + S602) antibody [EPR2471(2)] (AB134097), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPIHC-PWBFlow Cyt (Intra)
Human
Not recommended
Not recommended
Not recommended
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Predicted
Predicted
Rat
Not recommended
Not recommended
Not recommended
Predicted
Predicted

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000.00000 - 1/10000.00000
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN.

Additional Targets

PAK6 phospho S602, PAK5 phospho S560

Alternative names

KIAA1142, PAK4, Serine/threonine-protein kinase PAK 4, p21-activated kinase 4, PAK-4

Recommended products

Rabbit Recombinant Monoclonal PAK4 phospho S474 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR2471(2)
Purification technique
Affinity purification Protein A
Specificity

This antibody only detects PAK4 phosphorylated at Serine 474, PAK5 phosphorylated at Serine 602, and PAK6 phosphorylated at Serine 560.

Dissociation constant
4 x 10-12 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Flow Cytometry (Intracellular) - Anti-PAK4 + PAK5 + PAK6 (phospho S474 + S560 + S602) antibody [EPR2471(2)] (ab134097), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PAK4 + PAK5 + PAK6 (phospho S474 + S560 + S602) antibody [EPR2471(2)] (ab134097)

    Overlay histogram showing Hela cells stained with ab134097 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134097, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Western blot - Anti-PAK4 + PAK5 + PAK6 (phospho S474 + S560 + S602) antibody [EPR2471(2)] (ab134097), expandable thumbnail

    Western blot - Anti-PAK4 + PAK5 + PAK6 (phospho S474 + S560 + S602) antibody [EPR2471(2)] (ab134097)

    PAK4 + PAK5 + PAK6 (phospho S474 + S560 + S602) Western blot staining using rabbit Anti-PAK4 + PAK5 + PAK6 (phospho S474 + S560 + S602) antibody

    All lanes: Western blot - Anti-PAK4 + PAK5 + PAK6 (phospho S474 + S560 + S602) antibody [EPR2471(2)] (ab134097) at 1/1000 dilution

    Lane 1: HeLa cell lysate untreated at 10 µg

    Lane 2: HeLa cell lysate treated with Lambda Phosphatase at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 64 kDa

  • OI-RD Scanning - Anti-PAK4 + PAK5 + PAK6 (phospho S474 + S560 + S602) antibody [EPR2471(2)] (ab134097), expandable thumbnail

    OI-RD Scanning - Anti-PAK4 + PAK5 + PAK6 (phospho S474 + S560 + S602) antibody [EPR2471(2)] (ab134097)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com