AB110069

Anti-PAK5 antibody

Name: Anti-PAK5 antibody (ab110069) | Abcam
Brand: Abcam Limited
SKU: AB110069
Price: 535 USD
Availability: InStock

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(11 Publications)

Rabbit Polyclonal PAK5 antibody. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human PAK5.

View Alternative Names

KIAA1264, PAK7, PAK5, Serine/threonine-protein kinase PAK 5, p21-activated kinase 5, p21-activated kinase 7, PAK-5, PAK-7

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK5 antibody (AB110069)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK5 antibody (AB110069)

Immunohistochemical analysis of paraffin embedded Human brain tissue, using a 1/50 dilution of ab110069 with (right) or without (left) the immunizing peptide.

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Western blot - Anti-PAK5 antibody (AB110069)

All lanes:

Western blot - Anti-PAK5 antibody (ab110069) at 1/500 dilution

Lane 1:

extracts from Rat brain cells at 30 µg

Lane 2:

extracts from Rat brain cells at 30 µg with immunizing peptide

Predicted band size: 81 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human PAK5. The exact immunogen used to generate this antibody is proprietary information.

Q9P286

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab110069 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates the proto-oncogene RAF1 and stimulates its kinase activity. Promotes cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Phosphorylates CTNND1, probably to regulate cytoskeletal organization and cell morphology. Keeps microtubules stable through MARK2 inhibition and destabilizes the F-actin network leading to the disappearance of stress fibers and focal adhesions.

See full target information PAK5

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Brain : a journal of neurology 147:1809-1821 PubMed38079473

2023

PAK2 is necessary for myelination in the peripheral nervous system.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Hu,Daniel Moiseev,Isabella Schena,Bulat Faezov,Roland Dunbrack,Jonathan Chernoff,Jun Li

Neoplasma 67:333-343 PubMed31973536

2020

Long non-coding RNA SOX21-AS1 promotes cell proliferation and invasion through upregulating PAK7 expression by sponging miR-144-3p in glioma cells.

Applications

Unspecified application

Species

Unspecified reactive species

S Y Gai,Z H Yuan

Oncology reports 42:2390-2401 PubMed31638257

2019

miR‑489 promotes apoptosis and inhibits invasiveness of glioma cells by targeting PAK5/RAF1 signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wang,Luyang Zhang,Wei Gao,Dongyong Zhang,Zilong Zhao,Yijun Bao

Scientific reports 9:14436 PubMed31594963

2019

Normal mammary gland development after MMTV-Cre mediated conditional PAK4 gene depletion.

Applications

Unspecified application

Species

Unspecified reactive species

Parisa Rabieifar,Ting Zhuang,Tânia D F Costa,Miao Zhao,Staffan Strömblad

Oncology letters 15:2202-2210 PubMed29434926

2018

PAK5 overexpression is associated with lung metastasis in osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Han,Yan Zhou,Kuo-Fu Tseng,Haiyan Hu,Kunpeng Li,Yaling Wang,Zhihua Gan,Shuchen Lin,Yongning Sun,Daliu Min

Cell death & disease 8:e3155 PubMed29072688

2017

MiR-106a-5p inhibits the cell migration and invasion of renal cell carcinoma through targeting PAK5.

Applications

Species

Human

Yao-Jie Pan,Lu-Lu Wei,Xiao-Jin Wu,Fu-Chun Huo,Jie Mou,Dong-Sheng Pei

Oncology letters 14:404-410 PubMed28693183

2017

High expression of P21-activated kinase 5 protein is associated with poor survival in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tomoki Aburatani,Mikito Inokuchi,Yoko Takagi,Toshiaki Ishikawa,Keisuke Okuno,Kentaro Gokita,Chiharu Tomii,Toshiro Tanioka,Hideaki Murase,Sho Otsuki,Hiroyuki Uetake,Kazuyuki Kojima,Tatsuyuki Kawano

The Biochemical journal 473:1777-89 PubMed27095851

2016

PAK5 is auto-activated by a central domain that promotes kinase oligomerization.

Applications

Unspecified application

Species

Unspecified reactive species

Bahareh Tabanifar,Zhuoshen Zhao,Ed Manser

International journal of biological sciences 11:230-7 PubMed25632266

2015

Efficient inhibition of human glioma development by RNA interference-mediated silencing of PAK5.

Applications

IHC, WB

Species

Unspecified reactive species, Unspecified reactive species

Xuefeng Gu,Ce Wang,Xuefeng Wang,Guoda Ma,You Li,Lili Cui,Yanyan Chen,Bin Zhao,Keshen Li

Cell biology international 38:1394-402 PubMed25052921

2014

p21-activated kinase 7 is an oncogene in human osteosarcoma.

Applications

IHC

Species

Unspecified reactive species

Kun Han,Yan Zhou,Zhi-Hua Gan,Wei-Xiang Qi,Jian-Jun Zhang,Tao Fen,Wei Meng,Ling Jiang,Zan Shen,Da-Liu Min

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PAK5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK5 antibody (AB110069)

Western blot - Anti-PAK5 antibody (AB110069)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (11)

Product promise